Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers
We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal ce...
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| Veröffentlicht in: | Scientific reports 2019-07, Vol.9 (1), p.9885-13, Article 9885 |
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| creator | Duś-Szachniewicz, Kamila Drobczyński, Sławomir Woźniak, Marta Zduniak, Krzysztof Ostasiewicz, Katarzyna Ziółkowski, Piotr Korzeniewska, Aleksandra K. Agrawal, Anil K. Kołodziej, Paweł Walaszek, Kinga Bystydzieński, Zbigniew Rymkiewicz, Grzegorz |
| description | We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p |
| doi_str_mv | 10.1038/s41598-019-46086-y |
| format | Article |
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Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-46086-y</identifier><identifier>PMID: 31285461</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/106 ; 13/31 ; 639/624/1107/1110 ; 692/699/1541/1990/291/1621/1915 ; Adhesion ; Adhesives ; Adult ; Aged ; Aged, 80 and over ; B-Lymphocytes - pathology ; Biopsy ; Cell adhesion ; Cell adhesion & migration ; Cell Adhesion - physiology ; Cell Differentiation - physiology ; Female ; Flow Cytometry - methods ; Heterogeneity ; Humanities and Social Sciences ; Humans ; Lymphocytes B ; Lymphoma ; Lymphoma, B-Cell - pathology ; Lymphoma, Non-Hodgkin - pathology ; Male ; Mesenchymal stem cells ; Mesenchymal Stem Cells - pathology ; Mesenchyme ; Middle Aged ; multidisciplinary ; Non-Hodgkin's lymphoma ; Optical Tweezers ; Science ; Science (multidisciplinary) ; Stromal cells ; Stromal Cells - pathology ; Young Adult</subject><ispartof>Scientific reports, 2019-07, Vol.9 (1), p.9885-13, Article 9885</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity.</description><subject>13</subject><subject>13/106</subject><subject>13/31</subject><subject>639/624/1107/1110</subject><subject>692/699/1541/1990/291/1621/1915</subject><subject>Adhesion</subject><subject>Adhesives</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>B-Lymphocytes - pathology</subject><subject>Biopsy</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Female</subject><subject>Flow Cytometry - methods</subject><subject>Heterogeneity</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Lymphocytes B</subject><subject>Lymphoma</subject><subject>Lymphoma, B-Cell - pathology</subject><subject>Lymphoma, Non-Hodgkin - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duś-Szachniewicz, Kamila</au><au>Drobczyński, Sławomir</au><au>Woźniak, Marta</au><au>Zduniak, Krzysztof</au><au>Ostasiewicz, Katarzyna</au><au>Ziółkowski, Piotr</au><au>Korzeniewska, Aleksandra K.</au><au>Agrawal, Anil K.</au><au>Kołodziej, Paweł</au><au>Walaszek, Kinga</au><au>Bystydzieński, Zbigniew</au><au>Rymkiewicz, Grzegorz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-07-08</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>9885</spage><epage>13</epage><pages>9885-13</pages><artnum>9885</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>We have adapted a non-invasive method based on optical tweezers technology to differentiate between the normal B-cells and the B-cell non-Hodgkin lymphoma (B-NHL) cells derived from clinical samples. Our approach bases on the nascent adhesion between an individual B-cell and a mesenchymal stromal cell. In this study, a single B-cell was trapped and optically seeded on a mesenchymal stromal cell and kept in a direct contact with it until a stable connection between the cells was formed in time scale. This approach allowed us to avoid the introduction of any exogenous beads or chemicals into the experimental setup which would have affected the cell-to-cell adhesion. Here, we have provided new evidence that aberrant adhesive properties found in transformed B-cells are related to malignant neoplasia. We have demonstrated that the mean time required for establishing adhesive interactions between an individual normal B-cell and a mesenchymal stromal cell was 26.7 ± 16.6 s, while for lymphoma cell it was 208.8 ± 102.3 s, p < 0.001. The contact time for adhesion to occur ranged from 5 to 90 s and from 60 to 480 s for normal B-cells and lymphoma cells, respectively. This method for optically controlled cell-to-cell adhesion in time scale is beneficial to the successful differentiation of pathological cells from normal B-cells within the fine needle aspiration biopsy of a clinical sample. Additionally, variations in time-dependent adhesion among subtypes of B-NHL, established here by the optical trapping, confirm earlier results pertaining to cell heterogeneity.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31285461</pmid><doi>10.1038/s41598-019-46086-y</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9005-9695</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/106 13/31 639/624/1107/1110 692/699/1541/1990/291/1621/1915 Adhesion Adhesives Adult Aged Aged, 80 and over B-Lymphocytes - pathology Biopsy Cell adhesion Cell adhesion & migration Cell Adhesion - physiology Cell Differentiation - physiology Female Flow Cytometry - methods Heterogeneity Humanities and Social Sciences Humans Lymphocytes B Lymphoma Lymphoma, B-Cell - pathology Lymphoma, Non-Hodgkin - pathology Male Mesenchymal stem cells Mesenchymal Stem Cells - pathology Mesenchyme Middle Aged multidisciplinary Non-Hodgkin's lymphoma Optical Tweezers Science Science (multidisciplinary) Stromal cells Stromal Cells - pathology Young Adult |
| title | Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers |
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