Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation

We developed a reporter system that can be used in a dual manner in visualizing mature osteoblast formation. The system is based on a helper-dependent adenoviral vector (HDAdV), in which a fluorescent protein, Venus, is expressed under the control of the 19-kb human osteocalcin ( OC ) genomic locus....

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Veröffentlicht in:	Scientific reports 2019-07, Vol.9 (1), p.9705-10, Article 9705
Hauptverfasser:	Sone, Takefumi, Shin, Masashi, Ouchi, Takehito, Sasanuma, Hiroki, Miyamoto, Arei, Ohte, Satoshi, Tsukamoto, Sho, Nakanishi, Mahito, Okano, Hideyuki, Katagiri, Takenobu, Mitani, Kohnosuke
Format:	Artikel
Sprache:	eng
Schlagworte:	13/31 14/63 38/35 45/100 45/41 45/44 631/136/818 631/1647/245/2225 Adenoviridae - genetics Animals Artificial chromosomes Bone marrow Bone Neoplasms - metabolism Bone Neoplasms - pathology Cell culture Cell Differentiation Cloning Dentistry Drug dosages Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Flow cytometry Gene expression Genes, Reporter Genetic Vectors - administration & dosage Homologous recombination Humanities and Social Sciences Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Kinases Laboratory animals Medicine Mice Mice, Inbred C57BL multidisciplinary Neural crest Nodules Osteoblasts Osteoblasts - cytology Osteoblasts - metabolism Osteocalcin Osteogenesis Osteosarcoma - metabolism Osteosarcoma - pathology Pluripotency Research centers Science Science (multidisciplinary) Stem cell transplantation Stem cells
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