Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line
Radiation therapy (RT) represents a therapeutic option in breast cancer (BC). Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expre...
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| Veröffentlicht in: | Cancer genomics & proteomics 2019-07, Vol.16 (4), p.257-266 |
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| container_title | Cancer genomics & proteomics |
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| creator | Bravatà, Valentina Cammarata, Francesco Paolo Minafra, Luigi Musso, Rosa Pucci, Gaia Spada, Massimiliano Fazio, Ivan Russo, Giorgio Forte, Giusi Irma |
| description | Radiation therapy (RT) represents a therapeutic option in breast cancer (BC). Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expression profiles (GEP) induced by RT in triple-negative BC (TNBC) MDA-MB-231, to study signalling networks involved in radioresistance.
GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted.
A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance.
In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC. |
| doi_str_mv | 10.21873/cgp.20130 |
| format | Article |
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GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted.
A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance.
In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC.</description><identifier>ISSN: 1109-6535</identifier><identifier>EISSN: 1790-6245</identifier><identifier>DOI: 10.21873/cgp.20130</identifier><identifier>PMID: 31243106</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Background radiation ; Biotechnology ; Breast cancer ; Cell Line, Tumor ; DNA microarrays ; Female ; Gene expression ; Humans ; Ionizing radiation ; Menopause ; Morphology ; Radiation dosage ; Radiation therapy ; Radiation, Ionizing ; Radioresistance ; Signal transduction ; Transcription ; Transcriptome - genetics ; Triple Negative Breast Neoplasms - genetics ; Triple Negative Breast Neoplasms - pathology ; Triple Negative Breast Neoplasms - radiotherapy</subject><ispartof>Cancer genomics & proteomics, 2019-07, Vol.16 (4), p.257-266</ispartof><rights>Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.</rights><rights>Copyright International Institute of Anticancer Research Jul/Aug 2019</rights><rights>Copyright 2019, International Institute of Anticancer Research 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-9b8ddb29b59ab27fd453191454cabcb3bce4ba36caa17573a1ec106b8f5268413</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609258/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609258/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31243106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bravatà, Valentina</creatorcontrib><creatorcontrib>Cammarata, Francesco Paolo</creatorcontrib><creatorcontrib>Minafra, Luigi</creatorcontrib><creatorcontrib>Musso, Rosa</creatorcontrib><creatorcontrib>Pucci, Gaia</creatorcontrib><creatorcontrib>Spada, Massimiliano</creatorcontrib><creatorcontrib>Fazio, Ivan</creatorcontrib><creatorcontrib>Russo, Giorgio</creatorcontrib><creatorcontrib>Forte, Giusi Irma</creatorcontrib><title>Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line</title><title>Cancer genomics & proteomics</title><addtitle>Cancer Genomics Proteomics</addtitle><description>Radiation therapy (RT) represents a therapeutic option in breast cancer (BC). Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expression profiles (GEP) induced by RT in triple-negative BC (TNBC) MDA-MB-231, to study signalling networks involved in radioresistance.
GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted.
A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance.
In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC.</description><subject>Background radiation</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cell Line, Tumor</subject><subject>DNA microarrays</subject><subject>Female</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Ionizing radiation</subject><subject>Menopause</subject><subject>Morphology</subject><subject>Radiation dosage</subject><subject>Radiation therapy</subject><subject>Radiation, Ionizing</subject><subject>Radioresistance</subject><subject>Signal transduction</subject><subject>Transcription</subject><subject>Transcriptome - genetics</subject><subject>Triple Negative Breast Neoplasms - genetics</subject><subject>Triple Negative Breast Neoplasms - pathology</subject><subject>Triple Negative Breast Neoplasms - radiotherapy</subject><issn>1109-6535</issn><issn>1790-6245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUV1LHDEUDaXS1dWX_oAS6Fshms_5eCno1urCSovoc0gyd8YsYzJNZpfaX--sWtGne-Gce865HIQ-M3rMWVWKE9cNx5wyQT-gfVbWlBRcqo_TzmhNCiXUDB3kvKZUlkLST2gmGJeC0WIfbS8gAD7_OyTI2ceAf6fY-h4yXoZm46DB9gFf-u6ONDEDXsbg__nQ4WvTeDPuDnzAVz9OydUZ4YLhm-SHHkiAbkK3gM8SmDzihQkOEl5A3-OVD3CI9lrTZzh6mXN0-_P8ZnFJVr8ulovTFXGiFCOpbdU0ltdW1cbysm2kEqxmUklnrLPCOpDWiMIZw0pVCsPATW_ZqlW8qCQTc_T9WXfY2HtoHIQxmV4Pyd-b9KCj8fo9Evyd7uJWFwWtuaomga8vAin-2UAe9TpuUpgya84rxuTOZWJ9e2a5FHNO0L46MKqfOtJTR_qpo4n85W2mV-r_UsQjEqmM6Q</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Bravatà, Valentina</creator><creator>Cammarata, Francesco Paolo</creator><creator>Minafra, Luigi</creator><creator>Musso, Rosa</creator><creator>Pucci, Gaia</creator><creator>Spada, Massimiliano</creator><creator>Fazio, Ivan</creator><creator>Russo, Giorgio</creator><creator>Forte, Giusi Irma</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20190701</creationdate><title>Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line</title><author>Bravatà, Valentina ; 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Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expression profiles (GEP) induced by RT in triple-negative BC (TNBC) MDA-MB-231, to study signalling networks involved in radioresistance.
GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted.
A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance.
In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>31243106</pmid><doi>10.21873/cgp.20130</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Background radiation Biotechnology Breast cancer Cell Line, Tumor DNA microarrays Female Gene expression Humans Ionizing radiation Menopause Morphology Radiation dosage Radiation therapy Radiation, Ionizing Radioresistance Signal transduction Transcription Transcriptome - genetics Triple Negative Breast Neoplasms - genetics Triple Negative Breast Neoplasms - pathology Triple Negative Breast Neoplasms - radiotherapy |
| title | Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line |
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