Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells
The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin...
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| Veröffentlicht in: | International journal of molecular sciences 2019-06, Vol.20 (11), p.2753 |
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| creator | Li, Xin Diao, Min Zhang, Yanan Chen, Guanlin Huang, Shanjin Chen, Naizhi |
| description | The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called 'guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures. |
| doi_str_mv | 10.3390/ijms20112753 |
| format | Article |
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How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called 'guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20112753</identifier><identifier>PMID: 31195605</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Actin ; Apertures ; Arrays ; Bundles ; Cytoskeleton ; Depolymerization ; Filaments ; Guard cells ; Kinases ; Proteins ; Stomata</subject><ispartof>International journal of molecular sciences, 2019-06, Vol.20 (11), p.2753</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-44d35c7425c49961372071a0bca789be933a4b8e4bea80793b58e367c76671143</citedby><cites>FETCH-LOGICAL-c412t-44d35c7425c49961372071a0bca789be933a4b8e4bea80793b58e367c76671143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600335/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600335/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31195605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Diao, Min</creatorcontrib><creatorcontrib>Zhang, Yanan</creatorcontrib><creatorcontrib>Chen, Guanlin</creatorcontrib><creatorcontrib>Huang, Shanjin</creatorcontrib><creatorcontrib>Chen, Naizhi</creatorcontrib><title>Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. 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By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures.</description><subject>Actin</subject><subject>Apertures</subject><subject>Arrays</subject><subject>Bundles</subject><subject>Cytoskeleton</subject><subject>Depolymerization</subject><subject>Filaments</subject><subject>Guard cells</subject><subject>Kinases</subject><subject>Proteins</subject><subject>Stomata</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU9PGzEQxa0KVCjtrWdkiQuHpvjvOr5UikJDK4G4lLM163XA0a4d7F2k5BvwreuQQFNOntH89PxmHkJfKfnOuSYXftFlRihlSvIP6JgKxkaEVOpgrz5Cn3JeEMI4k_ojOuKUalkReYyerwZIDZ66tsU33qY49y10LvR4EqBdrV3CM7C-9T30LuP-wW0H2Wcc5y_9bbqH4NfQ-xgwhAZfrgJ03r4AE9v7gGc70YxLM0lQ-yYuNxL_fs-f0eEc2uy-7N4TdDf7-Wf6a3R9e_V7OrkeWUFZPxKi4dIqwaQVWleUK0YUBVJbUGNdO805iHrsRO1gTJTmtRw7XimrqkpRKvgJ-rHVXQ515xpbbCVozTL5DtLKRPDm_0nwD-Y-PpmqIoRzWQTOdwIpPg4u96bz2ZYVILg4ZMPKjblkiumCnr1DF3FI5X4bqtgXgmlSqG9bqpw_5-Tmb2YoMZuMzX7GBT_dX-ANfg2V_wWXhqNQ</recordid><startdate>20190605</startdate><enddate>20190605</enddate><creator>Li, Xin</creator><creator>Diao, Min</creator><creator>Zhang, Yanan</creator><creator>Chen, Guanlin</creator><creator>Huang, Shanjin</creator><creator>Chen, Naizhi</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190605</creationdate><title>Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells</title><author>Li, Xin ; Diao, Min ; Zhang, Yanan ; Chen, Guanlin ; Huang, Shanjin ; Chen, Naizhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-44d35c7425c49961372071a0bca789be933a4b8e4bea80793b58e367c76671143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Apertures</topic><topic>Arrays</topic><topic>Bundles</topic><topic>Cytoskeleton</topic><topic>Depolymerization</topic><topic>Filaments</topic><topic>Guard cells</topic><topic>Kinases</topic><topic>Proteins</topic><topic>Stomata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Diao, Min</creatorcontrib><creatorcontrib>Zhang, Yanan</creatorcontrib><creatorcontrib>Chen, Guanlin</creatorcontrib><creatorcontrib>Huang, Shanjin</creatorcontrib><creatorcontrib>Chen, Naizhi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xin</au><au>Diao, Min</au><au>Zhang, Yanan</au><au>Chen, Guanlin</au><au>Huang, Shanjin</au><au>Chen, Naizhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-06-05</date><risdate>2019</risdate><volume>20</volume><issue>11</issue><spage>2753</spage><pages>2753-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called 'guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. 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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Actin Apertures Arrays Bundles Cytoskeleton Depolymerization Filaments Guard cells Kinases Proteins Stomata |
| title | Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells |
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