A rapid bead-based radioligand binding assay for the determination of target-binding fraction and quality control of radiopharmaceuticals

Determination of the target-binding fraction (TBF) of radiopharmaceuticals using cell-based assays is prone to inconsistencies arising from several intrinsic and extrinsic factors. Here, we report a cell-free quantitative method of analysis to determine the TBF of radioligands. Magnetic beads functi...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nuclear medicine and biology 2019-04, Vol.71, p.32-38
Hauptverfasser:	Sharma, Sai Kiran, Lyashchenko, Serge K., Park, Hijin A., Pillarsetty, Nagavarakishore, Roux, Yorann, Wu, Jiong, Poty, Sophie, Tully, Kathryn M., Poirier, John T., Lewis, Jason S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Assay - methods Immunoreactivity Kinetics Ligands Lindmo assay Magnetic beads Quality Control Radiopharmaceuticals - chemistry Radiopharmaceuticals - metabolism Streptavidin - chemistry Streptavidin - metabolism Target-binding fraction
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!