Detecting the Presence of Bacterial DNA and RNA by Polymerase Chain Reaction to Diagnose Suspected Periprosthetic Joint Infection after Antibiotic Therapy

Objective To explore the diagnostic efficiency of DNA‐based and RNA‐based quantitative polymerase chain reaction (qPCR) analyses for periprosthetic joint infection (PJI). Methods To determine the detection limit of DNA‐based and RNA‐based qPCR in vitro, Staphylococcus aureus and Escherichia coli str...

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Veröffentlicht in:	Orthopaedic surgery 2018-02, Vol.10 (1), p.40-46
Hauptverfasser:	Fang, Xin‐yu, Li, Wen‐bo, Zhang, Chao‐fan, Huang, Zi‐da, Zeng, Hui‐yi, Dong, Zheng, Zhang, Wen‐ming
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Anti-Bacterial Agents - therapeutic use Antibiotic therapy Antibiotics Arthroplasty, Replacement, Knee Bacterial DNA Bacterial RNA Basic Research Deoxyribonucleic acid DNA DNA, Bacterial - analysis E coli Efficiency Escherichia coli - isolation & purification Escherichia coli Infections - diagnosis Female Humans Joint surgery Knee Prosthesis - adverse effects Male Middle Aged Osteoarthritis, Knee - surgery Periprosthetic joint infection Polymerase chain reaction Polymerase Chain Reaction - methods Postoperative Care - methods Prosthesis-Related Infections - diagnosis Prosthesis-Related Infections - microbiology Real‐time PCR RNA, Bacterial - analysis Sensitivity and Specificity Staphylococcal Infections - diagnosis Staphylococcus aureus - isolation & purification Synovial Fluid - microbiology
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