A fast and tuneable auxin‐inducible degron for depletion of target proteins in budding yeast

The auxin‐inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin‐binding receptor, TIR1, to target the AID‐tagged protein for degradation by...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2019-01, Vol.36 (1), p.75-81
Hauptverfasser:	Mendoza‐Ochoa, Gonzalo I., Barrass, J. David, Terlouw, Barbara R., Maudlin, Isabella E., Lucas, Susana, Sani, Emanuela, Aslanzadeh, Vahid, Reid, Jane A.E., Beggs, Jean D.
Format:	Artikel
Sprache:	eng
Schlagworte:	17β-Estradiol auxin auxins Biodegradation Cell culture degron Estradiol eukaryotic cells Fungal Proteins - genetics Gene Expression Gene Expression Regulation, Fungal gene induction Heat-Shock Proteins - genetics Indoleacetic Acids - metabolism Kinases Nuclear Proteins - genetics plant hormones proteasome endopeptidase complex Proteasomes protein depletion Protein Transport Proteins Proteolysis regulated gene expression Saccharomycetales - genetics Saccharomycetales - metabolism Special Issue Transcriptional Activation Yeast yeasts
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