Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality
The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reprodu...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2019-06, Vol.9 (1), p.8865-12, Article 8865 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 12 |
|---|---|
| container_issue | 1 |
| container_start_page | 8865 |
| container_title | Scientific reports |
| container_volume | 9 |
| creator | Zhang, Jiao Jiao Chandimali, Nisansala Kim, Nameun Kang, Tae Yoon Kim, Seong Bong Kim, Ji Su Wang, Xian Zhong Kwon, Taeho Jeong, Dong Kee |
| description | The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reproductive capacity in male chickens. In this study, we used the optimal plasma treatment condition (2.81 W for 2 min) which has been applied on 3.5-day-incubated fertilized eggs in the previous work and investigated the reproductive performance in male chickens aged at 20 and 40 weeks. The results showed that plasma exposure increased sperm count, motility, fertility rate, and fertilization period of male chickens. The sperm quality-promoting effect of plasma treatment was regulated by the significant improvements of adenosine triphosphate production and testosterone level, and by the modulation of reactive oxygen species balance and adenosine monophosphate-activated protein kinase and mammalian target of rapamycin pathway in the spermatozoa. Additionally, the plasma effect suggested that DNA demethylation and microRNA differential expression (a total number of 39 microRNAs were up-regulated whereas 53 microRNAs down-regulated in the testis) regulated the increases of adenosine triphosphate synthesis and testosterone level for promoting the chicken sperm quality. This finding might be beneficial to elevate the fertilization rate and embryo quality for the next generation in poultry breeding. |
| doi_str_mv | 10.1038/s41598-019-45087-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6586908</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2245607225</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-f52b0b90267c856caa9760d23281bd1eae257bee167f78ab2e50cb3acfe0012b3</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxa2qqCDgC3BAlrj0kmJP4vy5IK22hSKtQEJwthxnsuslsYOdoO63r5cFSnuoL7Y0v3kzz4-QE86-cZaW5yHjoioTxqskE6wsEv6JHADLRAIpwOcP731yHMKaxSOgynj1heynHABYBQfEfccex9WmU6Nxlirb0N5o7-5uZrQxbYse7WhUR_HX4DGELeRxOUUe6dCp0KvE2GbS2FDTD949Rz07UtdSvTL6ES0NA_qePk2qM-PmiOy1qgt4_HofkofLH_fzn8ni9up6PlskWnA-Jq2AmtUVg7zQpci1UlWRsybaKXndcFQIoqgReV60RalqQMF0nSrdImMc6vSQXOx0h6nusdFxJ686OXjTK7-RThn5d8WalVy6Z5mLMq9YGQW-vgp49zRhGGVvgsauUxbdFCRAJnJWAIiInv2Drt3kbbS3pTKeZqKASMGOir8bgsf2fRnO5DZSuYtUxkjlS6SSx6bTjzbeW94CjEC6A0Is2SX6P7P_I_sbuqevRg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2244134572</pqid></control><display><type>article</type><title>Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Zhang, Jiao Jiao ; Chandimali, Nisansala ; Kim, Nameun ; Kang, Tae Yoon ; Kim, Seong Bong ; Kim, Ji Su ; Wang, Xian Zhong ; Kwon, Taeho ; Jeong, Dong Kee</creator><creatorcontrib>Zhang, Jiao Jiao ; Chandimali, Nisansala ; Kim, Nameun ; Kang, Tae Yoon ; Kim, Seong Bong ; Kim, Ji Su ; Wang, Xian Zhong ; Kwon, Taeho ; Jeong, Dong Kee</creatorcontrib><description>The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reproductive capacity in male chickens. In this study, we used the optimal plasma treatment condition (2.81 W for 2 min) which has been applied on 3.5-day-incubated fertilized eggs in the previous work and investigated the reproductive performance in male chickens aged at 20 and 40 weeks. The results showed that plasma exposure increased sperm count, motility, fertility rate, and fertilization period of male chickens. The sperm quality-promoting effect of plasma treatment was regulated by the significant improvements of adenosine triphosphate production and testosterone level, and by the modulation of reactive oxygen species balance and adenosine monophosphate-activated protein kinase and mammalian target of rapamycin pathway in the spermatozoa. Additionally, the plasma effect suggested that DNA demethylation and microRNA differential expression (a total number of 39 microRNAs were up-regulated whereas 53 microRNAs down-regulated in the testis) regulated the increases of adenosine triphosphate synthesis and testosterone level for promoting the chicken sperm quality. This finding might be beneficial to elevate the fertilization rate and embryo quality for the next generation in poultry breeding.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-45087-1</identifier><identifier>PMID: 31222092</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/77 ; 38/90 ; 38/91 ; 631/136 ; 631/208 ; 82/80 ; Adenosine kinase ; Adenosine triphosphate ; Adenosine Triphosphate - blood ; AMP ; Animals ; ATP ; Chickens ; Demethylation ; Eggs ; Fertility ; Fertilization ; Humanities and Social Sciences ; Kinases ; Male ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; multidisciplinary ; Plasma ; Poultry ; Poultry production ; Protein kinase ; Quality ; Rapamycin ; Reactive oxygen species ; Reactive Oxygen Species - blood ; Reproduction ; Science ; Science (multidisciplinary) ; Sperm ; Sperm Count ; Sperm Motility ; Spermatozoa - cytology ; Testis - physiology ; Testosterone ; Testosterone - blood ; TOR protein</subject><ispartof>Scientific reports, 2019-06, Vol.9 (1), p.8865-12, Article 8865</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-f52b0b90267c856caa9760d23281bd1eae257bee167f78ab2e50cb3acfe0012b3</citedby><cites>FETCH-LOGICAL-c511t-f52b0b90267c856caa9760d23281bd1eae257bee167f78ab2e50cb3acfe0012b3</cites><orcidid>0000-0001-9489-0283 ; 0000-0002-2699-6729</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586908/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586908/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31222092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jiao Jiao</creatorcontrib><creatorcontrib>Chandimali, Nisansala</creatorcontrib><creatorcontrib>Kim, Nameun</creatorcontrib><creatorcontrib>Kang, Tae Yoon</creatorcontrib><creatorcontrib>Kim, Seong Bong</creatorcontrib><creatorcontrib>Kim, Ji Su</creatorcontrib><creatorcontrib>Wang, Xian Zhong</creatorcontrib><creatorcontrib>Kwon, Taeho</creatorcontrib><creatorcontrib>Jeong, Dong Kee</creatorcontrib><title>Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reproductive capacity in male chickens. In this study, we used the optimal plasma treatment condition (2.81 W for 2 min) which has been applied on 3.5-day-incubated fertilized eggs in the previous work and investigated the reproductive performance in male chickens aged at 20 and 40 weeks. The results showed that plasma exposure increased sperm count, motility, fertility rate, and fertilization period of male chickens. The sperm quality-promoting effect of plasma treatment was regulated by the significant improvements of adenosine triphosphate production and testosterone level, and by the modulation of reactive oxygen species balance and adenosine monophosphate-activated protein kinase and mammalian target of rapamycin pathway in the spermatozoa. Additionally, the plasma effect suggested that DNA demethylation and microRNA differential expression (a total number of 39 microRNAs were up-regulated whereas 53 microRNAs down-regulated in the testis) regulated the increases of adenosine triphosphate synthesis and testosterone level for promoting the chicken sperm quality. This finding might be beneficial to elevate the fertilization rate and embryo quality for the next generation in poultry breeding.</description><subject>38/77</subject><subject>38/90</subject><subject>38/91</subject><subject>631/136</subject><subject>631/208</subject><subject>82/80</subject><subject>Adenosine kinase</subject><subject>Adenosine triphosphate</subject><subject>Adenosine Triphosphate - blood</subject><subject>AMP</subject><subject>Animals</subject><subject>ATP</subject><subject>Chickens</subject><subject>Demethylation</subject><subject>Eggs</subject><subject>Fertility</subject><subject>Fertilization</subject><subject>Humanities and Social Sciences</subject><subject>Kinases</subject><subject>Male</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>multidisciplinary</subject><subject>Plasma</subject><subject>Poultry</subject><subject>Poultry production</subject><subject>Protein kinase</subject><subject>Quality</subject><subject>Rapamycin</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - blood</subject><subject>Reproduction</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sperm</subject><subject>Sperm Count</subject><subject>Sperm Motility</subject><subject>Spermatozoa - cytology</subject><subject>Testis - physiology</subject><subject>Testosterone</subject><subject>Testosterone - blood</subject><subject>TOR protein</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9P3DAQxa2qqCDgC3BAlrj0kmJP4vy5IK22hSKtQEJwthxnsuslsYOdoO63r5cFSnuoL7Y0v3kzz4-QE86-cZaW5yHjoioTxqskE6wsEv6JHADLRAIpwOcP731yHMKaxSOgynj1heynHABYBQfEfccex9WmU6Nxlirb0N5o7-5uZrQxbYse7WhUR_HX4DGELeRxOUUe6dCp0KvE2GbS2FDTD949Rz07UtdSvTL6ES0NA_qePk2qM-PmiOy1qgt4_HofkofLH_fzn8ni9up6PlskWnA-Jq2AmtUVg7zQpci1UlWRsybaKXndcFQIoqgReV60RalqQMF0nSrdImMc6vSQXOx0h6nusdFxJ686OXjTK7-RThn5d8WalVy6Z5mLMq9YGQW-vgp49zRhGGVvgsauUxbdFCRAJnJWAIiInv2Drt3kbbS3pTKeZqKASMGOir8bgsf2fRnO5DZSuYtUxkjlS6SSx6bTjzbeW94CjEC6A0Is2SX6P7P_I_sbuqevRg</recordid><startdate>20190620</startdate><enddate>20190620</enddate><creator>Zhang, Jiao Jiao</creator><creator>Chandimali, Nisansala</creator><creator>Kim, Nameun</creator><creator>Kang, Tae Yoon</creator><creator>Kim, Seong Bong</creator><creator>Kim, Ji Su</creator><creator>Wang, Xian Zhong</creator><creator>Kwon, Taeho</creator><creator>Jeong, Dong Kee</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9489-0283</orcidid><orcidid>https://orcid.org/0000-0002-2699-6729</orcidid></search><sort><creationdate>20190620</creationdate><title>Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality</title><author>Zhang, Jiao Jiao ; Chandimali, Nisansala ; Kim, Nameun ; Kang, Tae Yoon ; Kim, Seong Bong ; Kim, Ji Su ; Wang, Xian Zhong ; Kwon, Taeho ; Jeong, Dong Kee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-f52b0b90267c856caa9760d23281bd1eae257bee167f78ab2e50cb3acfe0012b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>38/77</topic><topic>38/90</topic><topic>38/91</topic><topic>631/136</topic><topic>631/208</topic><topic>82/80</topic><topic>Adenosine kinase</topic><topic>Adenosine triphosphate</topic><topic>Adenosine Triphosphate - blood</topic><topic>AMP</topic><topic>Animals</topic><topic>ATP</topic><topic>Chickens</topic><topic>Demethylation</topic><topic>Eggs</topic><topic>Fertility</topic><topic>Fertilization</topic><topic>Humanities and Social Sciences</topic><topic>Kinases</topic><topic>Male</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>multidisciplinary</topic><topic>Plasma</topic><topic>Poultry</topic><topic>Poultry production</topic><topic>Protein kinase</topic><topic>Quality</topic><topic>Rapamycin</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - blood</topic><topic>Reproduction</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sperm</topic><topic>Sperm Count</topic><topic>Sperm Motility</topic><topic>Spermatozoa - cytology</topic><topic>Testis - physiology</topic><topic>Testosterone</topic><topic>Testosterone - blood</topic><topic>TOR protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jiao Jiao</creatorcontrib><creatorcontrib>Chandimali, Nisansala</creatorcontrib><creatorcontrib>Kim, Nameun</creatorcontrib><creatorcontrib>Kang, Tae Yoon</creatorcontrib><creatorcontrib>Kim, Seong Bong</creatorcontrib><creatorcontrib>Kim, Ji Su</creatorcontrib><creatorcontrib>Wang, Xian Zhong</creatorcontrib><creatorcontrib>Kwon, Taeho</creatorcontrib><creatorcontrib>Jeong, Dong Kee</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jiao Jiao</au><au>Chandimali, Nisansala</au><au>Kim, Nameun</au><au>Kang, Tae Yoon</au><au>Kim, Seong Bong</au><au>Kim, Ji Su</au><au>Wang, Xian Zhong</au><au>Kwon, Taeho</au><au>Jeong, Dong Kee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-06-20</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>8865</spage><epage>12</epage><pages>8865-12</pages><artnum>8865</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reproductive capacity in male chickens. In this study, we used the optimal plasma treatment condition (2.81 W for 2 min) which has been applied on 3.5-day-incubated fertilized eggs in the previous work and investigated the reproductive performance in male chickens aged at 20 and 40 weeks. The results showed that plasma exposure increased sperm count, motility, fertility rate, and fertilization period of male chickens. The sperm quality-promoting effect of plasma treatment was regulated by the significant improvements of adenosine triphosphate production and testosterone level, and by the modulation of reactive oxygen species balance and adenosine monophosphate-activated protein kinase and mammalian target of rapamycin pathway in the spermatozoa. Additionally, the plasma effect suggested that DNA demethylation and microRNA differential expression (a total number of 39 microRNAs were up-regulated whereas 53 microRNAs down-regulated in the testis) regulated the increases of adenosine triphosphate synthesis and testosterone level for promoting the chicken sperm quality. This finding might be beneficial to elevate the fertilization rate and embryo quality for the next generation in poultry breeding.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31222092</pmid><doi>10.1038/s41598-019-45087-1</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9489-0283</orcidid><orcidid>https://orcid.org/0000-0002-2699-6729</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2019-06, Vol.9 (1), p.8865-12, Article 8865 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6586908 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 38/77 38/90 38/91 631/136 631/208 82/80 Adenosine kinase Adenosine triphosphate Adenosine Triphosphate - blood AMP Animals ATP Chickens Demethylation Eggs Fertility Fertilization Humanities and Social Sciences Kinases Male MicroRNAs MicroRNAs - genetics miRNA multidisciplinary Plasma Poultry Poultry production Protein kinase Quality Rapamycin Reactive oxygen species Reactive Oxygen Species - blood Reproduction Science Science (multidisciplinary) Sperm Sperm Count Sperm Motility Spermatozoa - cytology Testis - physiology Testosterone Testosterone - blood TOR protein |
| title | Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T21%3A47%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Demethylation%20and%20microRNA%20differential%20expression%20regulate%20plasma-induced%20improvement%20of%20chicken%20sperm%20quality&rft.jtitle=Scientific%20reports&rft.au=Zhang,%20Jiao%20Jiao&rft.date=2019-06-20&rft.volume=9&rft.issue=1&rft.spage=8865&rft.epage=12&rft.pages=8865-12&rft.artnum=8865&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-45087-1&rft_dat=%3Cproquest_pubme%3E2245607225%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2244134572&rft_id=info:pmid/31222092&rfr_iscdi=true |