Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients

Solid organ transplant (SOT) recipients are especially at risk of developing infections by multidrug resistant bacteria (MDR). In this study, the biofilm-forming capability of 209 MDR strains ( Escherichia coli n = 106, Klebsiella pneumoniae n = 78, and Enterobacter spp. n = 25) isolated from rectal...

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Veröffentlicht in:Scientific reports 2019-06, Vol.9 (1), p.8928-8928, Article 8928
Hauptverfasser: Ramos-Vivas, José, Chapartegui-González, Itziar, Fernández-Martínez, Marta, González-Rico, Claudia, Fortún, Jesús, Escudero, Rosa, Marco, Francesc, Linares, Laura, Montejo, Miguel, Aranzamendi, Maitane, Muñoz, Patricia, Valerio, Maricela, Aguado, Jose María, Resino, Elena, Ahufinger, Irene Gracia, Vega, Aurora Paz, Martínez-Martínez, Luis, Fariñas, María Carmen
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container_title Scientific reports
container_volume 9
creator Ramos-Vivas, José
Chapartegui-González, Itziar
Fernández-Martínez, Marta
González-Rico, Claudia
Fortún, Jesús
Escudero, Rosa
Marco, Francesc
Linares, Laura
Montejo, Miguel
Aranzamendi, Maitane
Muñoz, Patricia
Valerio, Maricela
Aguado, Jose María
Resino, Elena
Ahufinger, Irene Gracia
Vega, Aurora Paz
Martínez-Martínez, Luis
Fariñas, María Carmen
description Solid organ transplant (SOT) recipients are especially at risk of developing infections by multidrug resistant bacteria (MDR). In this study, the biofilm-forming capability of 209 MDR strains ( Escherichia coli n = 106, Klebsiella pneumoniae n = 78, and Enterobacter spp. n = 25) isolated from rectal swabs in the first 48 hours before or after kidney (93 patients), liver (60 patients) or kidney/pancreas transplants (5 patients) were evaluated by using a microplate assay. Thirty-nine strains were isolated before transplant and 170 strains were isolated post-transplant. Overall, 16% of E. coli strains, 73% of K. pneumoniae strains and 4% Enterobacter strains showed moderate or strong biofilm production. Nine strains isolated from infection sites after transplantation were responsible of infections in the first month. Of these, 4 K. pneumoniae , 1 E. coli and 1 Enterobacter spp. strains isolated pre-transplant or post-transplant as colonizers caused infections in the post-transplant period. Our results suggest that in vitro biofilm formation could be an important factor for adhesion to intestine and colonization in MDR K. pneumoniae strains in SOT recipients, but this factor appears to be less important for MDR E. coli and Enterobacter spp.
doi_str_mv 10.1038/s41598-019-45060-y
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In this study, the biofilm-forming capability of 209 MDR strains ( Escherichia coli n = 106, Klebsiella pneumoniae n = 78, and Enterobacter spp. n = 25) isolated from rectal swabs in the first 48 hours before or after kidney (93 patients), liver (60 patients) or kidney/pancreas transplants (5 patients) were evaluated by using a microplate assay. Thirty-nine strains were isolated before transplant and 170 strains were isolated post-transplant. Overall, 16% of E. coli strains, 73% of K. pneumoniae strains and 4% Enterobacter strains showed moderate or strong biofilm production. Nine strains isolated from infection sites after transplantation were responsible of infections in the first month. Of these, 4 K. pneumoniae , 1 E. coli and 1 Enterobacter spp. strains isolated pre-transplant or post-transplant as colonizers caused infections in the post-transplant period. 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subjects 14/34
631/326/107
692/308
96/63
Bacterial Infections - microbiology
Biofilms
Colonization
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
E coli
Enterobacter
Enterobacteriaceae - drug effects
Enterobacteriaceae - isolation & purification
Escherichia coli
Humanities and Social Sciences
Humans
Infections
Intestine
Kidney transplantation
Kidneys
Klebsiella pneumoniae
multidisciplinary
Multidrug resistance
Multidrug resistant organisms
Organ Transplantation
Pancreas transplantation
Rectum
Science
Science (multidisciplinary)
Strains (organisms)
Transplants & implants
title Biofilm formation by multidrug resistant Enterobacteriaceae strains isolated from solid organ transplant recipients
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A15%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biofilm%20formation%20by%20multidrug%20resistant%20Enterobacteriaceae%20strains%20isolated%20from%20solid%20organ%20transplant%20recipients&rft.jtitle=Scientific%20reports&rft.au=Ramos-Vivas,%20Jos%C3%A9&rft.aucorp=ENTHERE%20Study%20Group,%20the%20Group%20for%20Study%20of%20Infection%20in%20Transplantation%20of%20the%20Spanish%20Society%20of%20Infectious%20Diseases%20and%20Clinical%20Microbiology%20(GESITRA-SEIMC)%20and%20the%20Spanish%20Network%20for%20Research%20in%20Infectious%20Diseases%20(REIPI)&rft.date=2019-06-20&rft.volume=9&rft.issue=1&rft.spage=8928&rft.epage=8928&rft.pages=8928-8928&rft.artnum=8928&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-45060-y&rft_dat=%3Cproquest_pubme%3E2245607492%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2244134043&rft_id=info:pmid/31222089&rfr_iscdi=true