3D model for CAR‐mediated cytotoxicity using patient‐derived colorectal cancer organoids

Immunotherapy using chimeric antigen receptor (CAR)‐engineered lymphocytes has shown impressive results in leukemia. However, for solid tumors such as colorectal cancer (CRC), new preclinical models are needed that allow to test CAR‐mediated cytotoxicity in a tissue‐like environment. Here, we develo...

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Veröffentlicht in:	The EMBO journal 2019-06, Vol.38 (12), p.n/a
Hauptverfasser:	Schnalzger, Theresa E, de Groot, Marnix HP, Zhang, Congcong, Mosa, Mohammed H, Michels, Birgitta E, Röder, Jasmin, Darvishi, Tahmineh, Wels, Winfried S, Farin, Henner F
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Cancer Cancer immunotherapy CAR immunotherapy Cell culture Cell viability Cells, Cultured Chimeric antigen receptors Colon Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Colorectal Neoplasms - immunology Colorectal Neoplasms - pathology Colorectal Neoplasms - therapy Cytolytic activity Cytotoxicity cytotoxicity assays Cytotoxicity, Immunologic - drug effects Cytotoxicity, Immunologic - genetics Effector cells EMBO03 EMBO19 EMBO22 Extracellular matrix Frizzled protein Genetic Therapy - methods HEK293 Cells Humans Immunotherapy Immunotherapy, Adoptive - methods Intestine Leukemia Lymphocytes Lysis Model testing Models, Biological Natural killer cells Neoantigens Organoids Organoids - pathology patient‐derived organoids Primary Cell Culture - methods Receptors Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - metabolism Receptors, Antigen, T-Cell - therapeutic use Receptors, Chimeric Antigen - genetics Receptors, Chimeric Antigen - metabolism Receptors, Chimeric Antigen - therapeutic use Resource Side effects Solid tumors Subgroups Three dimensional models Tissue Culture Techniques - methods Tissue Scaffolds - chemistry Toxicity testing Tumors
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