Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix
Appicans are secreted or cell-associated brain chondroitin sulfate proteoglycans produced by glia cells and containing Alzheimer amyloid precursor protein (APP) as a core protein. Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appear...
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| Veröffentlicht in: | The Journal of neuroscience 1997-07, Vol.17 (13), p.4987-4993 |
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| creator | Wu, Anfan Pangalos, Menelas N Efthimiopoulos, Spiros Shioi, Junichi Robakis, Nikolaos K |
| description | Appicans are secreted or cell-associated brain chondroitin sulfate proteoglycans produced by glia cells and containing Alzheimer amyloid precursor protein (APP) as a core protein. Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appearance compared with untransfected cells or cells transfected with APP. Appican-transfected cells lost the round appearance of the untransfected control C6 cells, acquired a flat morphology, and elaborated more processes than control cells. Untransfected, or APP-transfected C6, cells were completely dissociated from their substrate after 40 min of treatment with cell dissociation solution. Under the same conditions, however, |
| doi_str_mv | 10.1523/jneurosci.17-13-04987.1997 |
| format | Article |
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Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appearance compared with untransfected cells or cells transfected with APP. Appican-transfected cells lost the round appearance of the untransfected control C6 cells, acquired a flat morphology, and elaborated more processes than control cells. Untransfected, or APP-transfected C6, cells were completely dissociated from their substrate after 40 min of treatment with cell dissociation solution. Under the same conditions, however, <20% of the appican-transfected C6 cells were dissociated from their substrate, suggesting that the appican-transfected glia cells attach more avidly to their substrate than do untransfected or APP transfected control cells. In contrast, appican-transfected fibroblast cells showed no morphological changes and dissociated from their substrate similarly to untransfected fibroblast cells. Extracellular matrix (ECM) prepared from appican-transfected C6 cell cultures contained high levels of appican and was a significantly better substrate for the attachment of C6 cells than ECM from either untransfected or APP-transfected cultures. Furthermore, cell adhesion to ECM was independent of the level of appican expression of the plated cells. ECM from appican-transfected C6 cultures stimulated adhesion of other neural cells including primary astrocytes, Neuro2a neuroblastoma, and PC12 pheochromocytoma, but not fibroblast cells. Conditioned media from appican-transfected C6 cultures failed to promote cell adhesion. Together, these data suggest that secreted appican incorporates into ECM and promotes adhesion of neural cells. Furthermore, our data suggest that the chondroitin sulfate chain engenders APP with novel biological functions.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.17-13-04987.1997</identifier><identifier>PMID: 9185536</identifier><language>eng</language><publisher>United States: Soc Neuroscience</publisher><subject>Amyloid beta-Protein Precursor - metabolism ; Animals ; Brain - metabolism ; Brain - pathology ; Cell Adhesion - physiology ; Extracellular Matrix - physiology ; Humans ; Isomerism ; Mice ; Neurons - physiology ; PC12 Cells ; Proteoglycans - metabolism ; Proteoglycans - physiology ; Rats ; Transfection ; Tumor Cells, Cultured</subject><ispartof>The Journal of neuroscience, 1997-07, Vol.17 (13), p.4987-4993</ispartof><rights>Copyright © 1997 Society for Neuroscience 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-eebf55cd19faafc7227e12c5ee044a287aa9ae7962a7b67d6c42429ad4a46733</citedby><cites>FETCH-LOGICAL-c550t-eebf55cd19faafc7227e12c5ee044a287aa9ae7962a7b67d6c42429ad4a46733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573314/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573314/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9185536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Anfan</creatorcontrib><creatorcontrib>Pangalos, Menelas N</creatorcontrib><creatorcontrib>Efthimiopoulos, Spiros</creatorcontrib><creatorcontrib>Shioi, Junichi</creatorcontrib><creatorcontrib>Robakis, Nikolaos K</creatorcontrib><title>Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Appicans are secreted or cell-associated brain chondroitin sulfate proteoglycans produced by glia cells and containing Alzheimer amyloid precursor protein (APP) as a core protein. Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appearance compared with untransfected cells or cells transfected with APP. Appican-transfected cells lost the round appearance of the untransfected control C6 cells, acquired a flat morphology, and elaborated more processes than control cells. Untransfected, or APP-transfected C6, cells were completely dissociated from their substrate after 40 min of treatment with cell dissociation solution. Under the same conditions, however, <20% of the appican-transfected C6 cells were dissociated from their substrate, suggesting that the appican-transfected glia cells attach more avidly to their substrate than do untransfected or APP transfected control cells. In contrast, appican-transfected fibroblast cells showed no morphological changes and dissociated from their substrate similarly to untransfected fibroblast cells. Extracellular matrix (ECM) prepared from appican-transfected C6 cell cultures contained high levels of appican and was a significantly better substrate for the attachment of C6 cells than ECM from either untransfected or APP-transfected cultures. Furthermore, cell adhesion to ECM was independent of the level of appican expression of the plated cells. ECM from appican-transfected C6 cultures stimulated adhesion of other neural cells including primary astrocytes, Neuro2a neuroblastoma, and PC12 pheochromocytoma, but not fibroblast cells. Conditioned media from appican-transfected C6 cultures failed to promote cell adhesion. Together, these data suggest that secreted appican incorporates into ECM and promotes adhesion of neural cells. Furthermore, our data suggest that the chondroitin sulfate chain engenders APP with novel biological functions.</description><subject>Amyloid beta-Protein Precursor - metabolism</subject><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Brain - pathology</subject><subject>Cell Adhesion - physiology</subject><subject>Extracellular Matrix - physiology</subject><subject>Humans</subject><subject>Isomerism</subject><subject>Mice</subject><subject>Neurons - physiology</subject><subject>PC12 Cells</subject><subject>Proteoglycans - metabolism</subject><subject>Proteoglycans - physiology</subject><subject>Rats</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhSMEGsrAIyBZLGCVYjtOHLNAqqIyFM0PgmFt3TpO45ETZ-yEDg_Ae-O01QhWrCzd853je3WS5A3BS5LT7P1dryfvgjJLwlOSpZiJki-JEPxJsoiESCnD5GmywJTjtGCcPU9ehHCHMeaY8LPkTJAyz7NikfxeDYNR0KP1w-B1CMb1aNPXk9IBXTk_tM66XQQsqlrod3FqelQV6MIa1wGqtLUBQV-jr951boz6qm71IcY16DruOVsP1OjQ2Or40ehBxclkwaMrGL15eJk8a8AG_er0nie3n9a31ef08uZiU60uU5XneEy13jZ5rmoiGoBGcUq5JlTlWmPGgJYcQIDmoqDAtwWvC8UoowJqBqzgWXaefDzGDtO207XSfVzFysGbDvwv6cDIf5XetHLnfsoij27CYsDbU4B395MOo-xMmG-BXrspSC5wmVEm_guSIiN5SXAEPxxBFQsNXjeP2xAs57Lll-v1j28336uNJFySTB7KlnPZ0fz673serad2o_7uqLdm1-6N1zJ0YG2kidzv98e8OS77A11PuOU</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Wu, Anfan</creator><creator>Pangalos, Menelas N</creator><creator>Efthimiopoulos, Spiros</creator><creator>Shioi, Junichi</creator><creator>Robakis, Nikolaos K</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix</title><author>Wu, Anfan ; Pangalos, Menelas N ; Efthimiopoulos, Spiros ; Shioi, Junichi ; Robakis, Nikolaos K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-eebf55cd19faafc7227e12c5ee044a287aa9ae7962a7b67d6c42429ad4a46733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amyloid beta-Protein Precursor - metabolism</topic><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Brain - pathology</topic><topic>Cell Adhesion - physiology</topic><topic>Extracellular Matrix - physiology</topic><topic>Humans</topic><topic>Isomerism</topic><topic>Mice</topic><topic>Neurons - physiology</topic><topic>PC12 Cells</topic><topic>Proteoglycans - metabolism</topic><topic>Proteoglycans - physiology</topic><topic>Rats</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Anfan</creatorcontrib><creatorcontrib>Pangalos, Menelas N</creatorcontrib><creatorcontrib>Efthimiopoulos, Spiros</creatorcontrib><creatorcontrib>Shioi, Junichi</creatorcontrib><creatorcontrib>Robakis, Nikolaos K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Anfan</au><au>Pangalos, Menelas N</au><au>Efthimiopoulos, Spiros</au><au>Shioi, Junichi</au><au>Robakis, Nikolaos K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>17</volume><issue>13</issue><spage>4987</spage><epage>4993</epage><pages>4987-4993</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Appicans are secreted or cell-associated brain chondroitin sulfate proteoglycans produced by glia cells and containing Alzheimer amyloid precursor protein (APP) as a core protein. Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appearance compared with untransfected cells or cells transfected with APP. Appican-transfected cells lost the round appearance of the untransfected control C6 cells, acquired a flat morphology, and elaborated more processes than control cells. Untransfected, or APP-transfected C6, cells were completely dissociated from their substrate after 40 min of treatment with cell dissociation solution. Under the same conditions, however, <20% of the appican-transfected C6 cells were dissociated from their substrate, suggesting that the appican-transfected glia cells attach more avidly to their substrate than do untransfected or APP transfected control cells. In contrast, appican-transfected fibroblast cells showed no morphological changes and dissociated from their substrate similarly to untransfected fibroblast cells. Extracellular matrix (ECM) prepared from appican-transfected C6 cell cultures contained high levels of appican and was a significantly better substrate for the attachment of C6 cells than ECM from either untransfected or APP-transfected cultures. Furthermore, cell adhesion to ECM was independent of the level of appican expression of the plated cells. ECM from appican-transfected C6 cultures stimulated adhesion of other neural cells including primary astrocytes, Neuro2a neuroblastoma, and PC12 pheochromocytoma, but not fibroblast cells. Conditioned media from appican-transfected C6 cultures failed to promote cell adhesion. Together, these data suggest that secreted appican incorporates into ECM and promotes adhesion of neural cells. Furthermore, our data suggest that the chondroitin sulfate chain engenders APP with novel biological functions.</abstract><cop>United States</cop><pub>Soc Neuroscience</pub><pmid>9185536</pmid><doi>10.1523/jneurosci.17-13-04987.1997</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amyloid beta-Protein Precursor - metabolism Animals Brain - metabolism Brain - pathology Cell Adhesion - physiology Extracellular Matrix - physiology Humans Isomerism Mice Neurons - physiology PC12 Cells Proteoglycans - metabolism Proteoglycans - physiology Rats Transfection Tumor Cells, Cultured |
| title | Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix |
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