Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells
Glia maturation factor-beta (GMF-beta) is a 17 kDa protein purified and sequenced from bovine brains. Using the monoclonal antibody G2-09 directed against GMF-beta, we previously demonstrated endogenous GMF-beta in astroblasts, Schwann cells, and their tumors in culture. In the present study, we hav...
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| Veröffentlicht in: | The Journal of neuroscience 1989-10, Vol.9 (10), p.3690-3698 |
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| creator | Bosch, EP Zhong, W Lim, R |
| description | Glia maturation factor-beta (GMF-beta) is a 17 kDa protein purified and sequenced from bovine brains. Using the monoclonal antibody G2-09 directed against GMF-beta, we previously demonstrated endogenous GMF-beta in astroblasts, Schwann cells, and their tumors in culture. In the present study, we have used indirect immunofluorescence microscopy with G2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of GMF-beta in Schwann cells in situ and to study the time course of GMF-beta induction in Schwann cells in vitro. For comparison, a parallel study was carried out with monoclonal antibodies directed against nerve growth factor (NGF) receptor. We found that (1) neither GMF-beta nor NGF receptor was detectable in intact sciatic nerves, (2) all Schwann cells of the distal segment of the transected nerve expressed GMF-beta as early as 3 d after axotomy that persisted up to 3 weeks, (3) axonal regeneration repressed the Schwann cell expression of GMF-beta, (4) isolated Schwann cells derived from rat sciatic and adult human sural nerves developed intracellular GMF-beta in culture following an initial lag period, and (5) the induction of Schwann cell NGF receptor coincided temporally with that of GMF-beta in the transected nerve and in culture. These results show that the expression of GMF-beta in Schwann cells, as is the case with the NGF receptor, is induced by the loss of the normal axon-Schwann cell contact. We propose that the induction of GMF-beta, as well as NGF receptor, in Schwann cells after nerve injury plays a role in axonal regeneration. |
| doi_str_mv | 10.1523/jneurosci.09-10-03690.1989 |
| format | Article |
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Using the monoclonal antibody G2-09 directed against GMF-beta, we previously demonstrated endogenous GMF-beta in astroblasts, Schwann cells, and their tumors in culture. In the present study, we have used indirect immunofluorescence microscopy with G2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of GMF-beta in Schwann cells in situ and to study the time course of GMF-beta induction in Schwann cells in vitro. For comparison, a parallel study was carried out with monoclonal antibodies directed against nerve growth factor (NGF) receptor. We found that (1) neither GMF-beta nor NGF receptor was detectable in intact sciatic nerves, (2) all Schwann cells of the distal segment of the transected nerve expressed GMF-beta as early as 3 d after axotomy that persisted up to 3 weeks, (3) axonal regeneration repressed the Schwann cell expression of GMF-beta, (4) isolated Schwann cells derived from rat sciatic and adult human sural nerves developed intracellular GMF-beta in culture following an initial lag period, and (5) the induction of Schwann cell NGF receptor coincided temporally with that of GMF-beta in the transected nerve and in culture. These results show that the expression of GMF-beta in Schwann cells, as is the case with the NGF receptor, is induced by the loss of the normal axon-Schwann cell contact. We propose that the induction of GMF-beta, as well as NGF receptor, in Schwann cells after nerve injury plays a role in axonal regeneration.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/jneurosci.09-10-03690.1989</identifier><identifier>PMID: 2795149</identifier><language>eng</language><publisher>United States: Soc Neuroscience</publisher><subject>Animals ; Axons - physiology ; Cells, Cultured ; Female ; Glia Maturation Factor ; Growth Substances - metabolism ; Immunohistochemistry ; Nerve Crush ; Nerve Regeneration ; Nerve Tissue Proteins - metabolism ; Rats ; Rats, Inbred Strains ; Schwann Cells - metabolism ; Schwann Cells - physiology ; Sciatic Nerve - cytology ; Sciatic Nerve - metabolism ; Sciatic Nerve - physiology ; Tissue Distribution</subject><ispartof>The Journal of neuroscience, 1989-10, Vol.9 (10), p.3690-3698</ispartof><rights>1989 by Society for Neuroscience 1989</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-c6c7601559ee3f2d8eec4553a42c3de392752b5a66fdcaf8f2d2b66fef0a2dc23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6569890/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6569890/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2795149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bosch, EP</creatorcontrib><creatorcontrib>Zhong, W</creatorcontrib><creatorcontrib>Lim, R</creatorcontrib><title>Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Glia maturation factor-beta (GMF-beta) is a 17 kDa protein purified and sequenced from bovine brains. Using the monoclonal antibody G2-09 directed against GMF-beta, we previously demonstrated endogenous GMF-beta in astroblasts, Schwann cells, and their tumors in culture. In the present study, we have used indirect immunofluorescence microscopy with G2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of GMF-beta in Schwann cells in situ and to study the time course of GMF-beta induction in Schwann cells in vitro. For comparison, a parallel study was carried out with monoclonal antibodies directed against nerve growth factor (NGF) receptor. We found that (1) neither GMF-beta nor NGF receptor was detectable in intact sciatic nerves, (2) all Schwann cells of the distal segment of the transected nerve expressed GMF-beta as early as 3 d after axotomy that persisted up to 3 weeks, (3) axonal regeneration repressed the Schwann cell expression of GMF-beta, (4) isolated Schwann cells derived from rat sciatic and adult human sural nerves developed intracellular GMF-beta in culture following an initial lag period, and (5) the induction of Schwann cell NGF receptor coincided temporally with that of GMF-beta in the transected nerve and in culture. These results show that the expression of GMF-beta in Schwann cells, as is the case with the NGF receptor, is induced by the loss of the normal axon-Schwann cell contact. We propose that the induction of GMF-beta, as well as NGF receptor, in Schwann cells after nerve injury plays a role in axonal regeneration.</description><subject>Animals</subject><subject>Axons - physiology</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Glia Maturation Factor</subject><subject>Growth Substances - metabolism</subject><subject>Immunohistochemistry</subject><subject>Nerve Crush</subject><subject>Nerve Regeneration</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Schwann Cells - metabolism</subject><subject>Schwann Cells - physiology</subject><subject>Sciatic Nerve - cytology</subject><subject>Sciatic Nerve - metabolism</subject><subject>Sciatic Nerve - physiology</subject><subject>Tissue Distribution</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc9O3DAQxq2qFV0oj1DJ6qG3gOPEyZpDJbSiFISKVOBseZ1J4lVib_2HwAP1PessK1pOI89885sZfwh9yclJzmhxujEQnfVKnxCe5SQjRcVTiS_5O7RICp7RkuTv0YLQmmRVWZcf0aH3G0JITfL6AB3QmrO85Av05_zJGjlgr7sUPHbQxUEGwPC0deC9tgbbFneDlniUIToZ5lQrVbAuW0OQWBt8p_pJGoMVDIM_w9JgPY7R2F77YFUPo1bzjBCb55mmzSY6aHC6IOEUNuAewae2Bqs4hF3tDfIT-tCm7eB4H4_Qw_eL-9WP7Ob28mp1fpOpktGQqUrVFckZ4wBFS5slQCqwQpZUFQ0UnNaMrpmsqrZRsl0mCV2nB7RE0kbR4gh9e-Fu43qERoEJTg5i6_Qo3bOwUou3FaN70dlHUbEq_T5JgK97gLO_I_ggRu3nE6QBG72oOS1pyVkSnr0IVTLSO2hfh-REzCaL658XD79u71ZXgvA5uTNZzCan5s__r_naunf13xa97vpJOxB-lMOQ1LmYpmmHm2nFX-3Wud0</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Bosch, EP</creator><creator>Zhong, W</creator><creator>Lim, R</creator><general>Soc Neuroscience</general><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19891001</creationdate><title>Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells</title><author>Bosch, EP ; Zhong, W ; Lim, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-c6c7601559ee3f2d8eec4553a42c3de392752b5a66fdcaf8f2d2b66fef0a2dc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Axons - physiology</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Glia Maturation Factor</topic><topic>Growth Substances - metabolism</topic><topic>Immunohistochemistry</topic><topic>Nerve Crush</topic><topic>Nerve Regeneration</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Schwann Cells - metabolism</topic><topic>Schwann Cells - physiology</topic><topic>Sciatic Nerve - cytology</topic><topic>Sciatic Nerve - metabolism</topic><topic>Sciatic Nerve - physiology</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bosch, EP</creatorcontrib><creatorcontrib>Zhong, W</creatorcontrib><creatorcontrib>Lim, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bosch, EP</au><au>Zhong, W</au><au>Lim, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells</atitle><jtitle>The Journal of neuroscience</jtitle><addtitle>J Neurosci</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>9</volume><issue>10</issue><spage>3690</spage><epage>3698</epage><pages>3690-3698</pages><issn>0270-6474</issn><eissn>1529-2401</eissn><abstract>Glia maturation factor-beta (GMF-beta) is a 17 kDa protein purified and sequenced from bovine brains. Using the monoclonal antibody G2-09 directed against GMF-beta, we previously demonstrated endogenous GMF-beta in astroblasts, Schwann cells, and their tumors in culture. In the present study, we have used indirect immunofluorescence microscopy with G2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of GMF-beta in Schwann cells in situ and to study the time course of GMF-beta induction in Schwann cells in vitro. For comparison, a parallel study was carried out with monoclonal antibodies directed against nerve growth factor (NGF) receptor. We found that (1) neither GMF-beta nor NGF receptor was detectable in intact sciatic nerves, (2) all Schwann cells of the distal segment of the transected nerve expressed GMF-beta as early as 3 d after axotomy that persisted up to 3 weeks, (3) axonal regeneration repressed the Schwann cell expression of GMF-beta, (4) isolated Schwann cells derived from rat sciatic and adult human sural nerves developed intracellular GMF-beta in culture following an initial lag period, and (5) the induction of Schwann cell NGF receptor coincided temporally with that of GMF-beta in the transected nerve and in culture. These results show that the expression of GMF-beta in Schwann cells, as is the case with the NGF receptor, is induced by the loss of the normal axon-Schwann cell contact. We propose that the induction of GMF-beta, as well as NGF receptor, in Schwann cells after nerve injury plays a role in axonal regeneration.</abstract><cop>United States</cop><pub>Soc Neuroscience</pub><pmid>2795149</pmid><doi>10.1523/jneurosci.09-10-03690.1989</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Axons - physiology Cells, Cultured Female Glia Maturation Factor Growth Substances - metabolism Immunohistochemistry Nerve Crush Nerve Regeneration Nerve Tissue Proteins - metabolism Rats Rats, Inbred Strains Schwann Cells - metabolism Schwann Cells - physiology Sciatic Nerve - cytology Sciatic Nerve - metabolism Sciatic Nerve - physiology Tissue Distribution |
| title | Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells |
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