Controlled outgrowth of dissociated neurons on patterned substrates

The cytoarchitecture of nervous tissue is lost during the dissociation procedures used to form primary cell cultures. As a first step toward reestablishing an ordered arrangement of these cells in vitro, we developed a set of procedures for patterning the outgrowth of cells cultured on 2-dimensional...

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Veröffentlicht in:	The Journal of neuroscience 1988-11, Vol.8 (11), p.4098-4120
Hauptverfasser:	Kleinfeld, D, Kahler, KH, Hockberger, PE
Format:	Artikel
Sprache:	eng
Schlagworte:	Amines - pharmacology Amino Acids - pharmacology Animal cells Animals Biological and medical sciences Cell Adhesion - drug effects Cell cultures. Hybridization. Fusion Cell Separation Cell Survival Cells, Cultured Cerebellum - cytology Cytological Techniques Electrophysiology Ethylenediamines - pharmacology Fundamental and applied biological sciences. Psychology Glial Fibrillary Acidic Protein - analysis Immunohistochemistry Mammalia Molecular and cellular biology Neurons - physiology Phosphopyruvate Hydratase - analysis Polyamines - pharmacology Propylamines - pharmacology Purkinje Cells - physiology Silanes - pharmacology Surface Properties
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