An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis )
The aim of this study was to determine how the mulberry ( ) polyphenol oxidase 1 gene ( ) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the -acting elements in the promoter. Then, we used t...
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| Veröffentlicht in: | International journal of molecular sciences 2019-05, Vol.20 (10), p.2602 |
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| description | The aim of this study was to determine how the mulberry (
) polyphenol oxidase 1 gene (
) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the
-acting elements in the
promoter. Then, we used the
promoter region [(1268 bp, including an MYB3R-binding
-element (MSA)] as a probe to capture proteins in DNA pull-down assays. These analyses revealed that the MYB3R1 transcription factor in
(encoded by
) binds to the
promoter region. We further explored the interaction between the
promoter and MYB3R1 with the dual luciferase reporter, yeast one-hybrid, and chromatin immunoprecipitation assays. These analyses verified that MnMYB3R1 binds to the MSA in the
promoter region. The overexpression of
in tobacco upregulated the expression of the tobacco
gene. This observation as well as the quantitative real-time PCR results implied that
and
are involved in the abscisic acid-responsive stress response pathway. |
| doi_str_mv | 10.3390/ijms20102602 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6567046</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2332084988</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-8b9802e5d7944a76756362e2c13daa3bd72d29fdd0d9e5b10b7be394b450d14e3</originalsourceid><addsrcrecordid>eNpdkcFrFDEYxYNYbG29eZaAlwq7mnzJTGYuQi22Fbq0DPXgKSSTb7tZssk2mRH3v3ektayevgfvx-N7PELecvZRiJZ98utNAcYZ1AxekCMuAeaM1erlnj4kr0tZMwYCqvYVORScC9UodUTKWaQd76ATdPHjC73LJpY---3gU6QXph9SntFFnDzR8Rnt8H4MZsBChxXS2xR22xXGFOjNL-9MQXqJEamPdDEGiznv6CldpDwWGtNgrA--0A8n5GBpQsE3T_eYfL_4end-Nb--ufx2fnY97yWHYd7YtmGAlVOtlEbVqqpFDQg9F84YYZ0CB-3SOeZarCxnVlkUrbSyYo5LFMfk82PudrQbdD3GIZugt9lvTN7pZLz-14l-pe_TT11XtWKyngJOnwJyehixDHrjS48hmIhpLBpA8Ea2nFUT-v4_dJ3GHKd6GoQANmFNM1GzR6rPqZSMy-dnONN_1tT7a074u_0Cz_Df-cRvZDCZaQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2332084988</pqid></control><display><type>article</type><title>An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis )</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Liu, Dan ; Meng, Shuai ; Xiang, Zhonghuai ; Yang, Guangwei ; He, Ningjia</creator><creatorcontrib>Liu, Dan ; Meng, Shuai ; Xiang, Zhonghuai ; Yang, Guangwei ; He, Ningjia</creatorcontrib><description>The aim of this study was to determine how the mulberry (
) polyphenol oxidase 1 gene (
) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the
-acting elements in the
promoter. Then, we used the
promoter region [(1268 bp, including an MYB3R-binding
-element (MSA)] as a probe to capture proteins in DNA pull-down assays. These analyses revealed that the MYB3R1 transcription factor in
(encoded by
) binds to the
promoter region. We further explored the interaction between the
promoter and MYB3R1 with the dual luciferase reporter, yeast one-hybrid, and chromatin immunoprecipitation assays. These analyses verified that MnMYB3R1 binds to the MSA in the
promoter region. The overexpression of
in tobacco upregulated the expression of the tobacco
gene. This observation as well as the quantitative real-time PCR results implied that
and
are involved in the abscisic acid-responsive stress response pathway.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20102602</identifier><identifier>PMID: 31137877</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abiotic stress ; Anthocyanins ; Barriers ; Biotin ; Cell cycle ; Cell death ; Cell walls ; Crosslinking ; Deoxyribonucleic acid ; DNA ; Genes ; Hydrazine ; Hydrazines ; Hydrogen peroxide ; Hypotheses ; Mass spectrometry ; Medical research ; Melanin ; Morus notabilis ; Pathogens ; Phase transitions ; Phenolic compounds ; Phylogenetics ; Plant growth ; Plant resistance ; Polyacrylamide ; Polymers ; Polyphenol oxidase ; Polyphenols ; PPOs ; Proteins ; Quinones ; Ruthenium ; Scientific imaging ; Tobacco ; Transcription factors ; Tyramine ; Tyrosinase ; Tyrosine ; Yeast</subject><ispartof>International journal of molecular sciences, 2019-05, Vol.20 (10), p.2602</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8b9802e5d7944a76756362e2c13daa3bd72d29fdd0d9e5b10b7be394b450d14e3</citedby><cites>FETCH-LOGICAL-c412t-8b9802e5d7944a76756362e2c13daa3bd72d29fdd0d9e5b10b7be394b450d14e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567046/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567046/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31137877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Meng, Shuai</creatorcontrib><creatorcontrib>Xiang, Zhonghuai</creatorcontrib><creatorcontrib>Yang, Guangwei</creatorcontrib><creatorcontrib>He, Ningjia</creatorcontrib><title>An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis )</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The aim of this study was to determine how the mulberry (
) polyphenol oxidase 1 gene (
) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the
-acting elements in the
promoter. Then, we used the
promoter region [(1268 bp, including an MYB3R-binding
-element (MSA)] as a probe to capture proteins in DNA pull-down assays. These analyses revealed that the MYB3R1 transcription factor in
(encoded by
) binds to the
promoter region. We further explored the interaction between the
promoter and MYB3R1 with the dual luciferase reporter, yeast one-hybrid, and chromatin immunoprecipitation assays. These analyses verified that MnMYB3R1 binds to the MSA in the
promoter region. The overexpression of
in tobacco upregulated the expression of the tobacco
gene. This observation as well as the quantitative real-time PCR results implied that
and
are involved in the abscisic acid-responsive stress response pathway.</description><subject>Abiotic stress</subject><subject>Anthocyanins</subject><subject>Barriers</subject><subject>Biotin</subject><subject>Cell cycle</subject><subject>Cell death</subject><subject>Cell walls</subject><subject>Crosslinking</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Genes</subject><subject>Hydrazine</subject><subject>Hydrazines</subject><subject>Hydrogen peroxide</subject><subject>Hypotheses</subject><subject>Mass spectrometry</subject><subject>Medical research</subject><subject>Melanin</subject><subject>Morus notabilis</subject><subject>Pathogens</subject><subject>Phase transitions</subject><subject>Phenolic compounds</subject><subject>Phylogenetics</subject><subject>Plant growth</subject><subject>Plant resistance</subject><subject>Polyacrylamide</subject><subject>Polymers</subject><subject>Polyphenol oxidase</subject><subject>Polyphenols</subject><subject>PPOs</subject><subject>Proteins</subject><subject>Quinones</subject><subject>Ruthenium</subject><subject>Scientific imaging</subject><subject>Tobacco</subject><subject>Transcription factors</subject><subject>Tyramine</subject><subject>Tyrosinase</subject><subject>Tyrosine</subject><subject>Yeast</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkcFrFDEYxYNYbG29eZaAlwq7mnzJTGYuQi22Fbq0DPXgKSSTb7tZssk2mRH3v3ektayevgfvx-N7PELecvZRiJZ98utNAcYZ1AxekCMuAeaM1erlnj4kr0tZMwYCqvYVORScC9UodUTKWaQd76ATdPHjC73LJpY---3gU6QXph9SntFFnDzR8Rnt8H4MZsBChxXS2xR22xXGFOjNL-9MQXqJEamPdDEGiznv6CldpDwWGtNgrA--0A8n5GBpQsE3T_eYfL_4end-Nb--ufx2fnY97yWHYd7YtmGAlVOtlEbVqqpFDQg9F84YYZ0CB-3SOeZarCxnVlkUrbSyYo5LFMfk82PudrQbdD3GIZugt9lvTN7pZLz-14l-pe_TT11XtWKyngJOnwJyehixDHrjS48hmIhpLBpA8Ea2nFUT-v4_dJ3GHKd6GoQANmFNM1GzR6rPqZSMy-dnONN_1tT7a074u_0Cz_Df-cRvZDCZaQ</recordid><startdate>20190527</startdate><enddate>20190527</enddate><creator>Liu, Dan</creator><creator>Meng, Shuai</creator><creator>Xiang, Zhonghuai</creator><creator>Yang, Guangwei</creator><creator>He, Ningjia</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190527</creationdate><title>An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis )</title><author>Liu, Dan ; Meng, Shuai ; Xiang, Zhonghuai ; Yang, Guangwei ; He, Ningjia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8b9802e5d7944a76756362e2c13daa3bd72d29fdd0d9e5b10b7be394b450d14e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abiotic stress</topic><topic>Anthocyanins</topic><topic>Barriers</topic><topic>Biotin</topic><topic>Cell cycle</topic><topic>Cell death</topic><topic>Cell walls</topic><topic>Crosslinking</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Genes</topic><topic>Hydrazine</topic><topic>Hydrazines</topic><topic>Hydrogen peroxide</topic><topic>Hypotheses</topic><topic>Mass spectrometry</topic><topic>Medical research</topic><topic>Melanin</topic><topic>Morus notabilis</topic><topic>Pathogens</topic><topic>Phase transitions</topic><topic>Phenolic compounds</topic><topic>Phylogenetics</topic><topic>Plant growth</topic><topic>Plant resistance</topic><topic>Polyacrylamide</topic><topic>Polymers</topic><topic>Polyphenol oxidase</topic><topic>Polyphenols</topic><topic>PPOs</topic><topic>Proteins</topic><topic>Quinones</topic><topic>Ruthenium</topic><topic>Scientific imaging</topic><topic>Tobacco</topic><topic>Transcription factors</topic><topic>Tyramine</topic><topic>Tyrosinase</topic><topic>Tyrosine</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Meng, Shuai</creatorcontrib><creatorcontrib>Xiang, Zhonghuai</creatorcontrib><creatorcontrib>Yang, Guangwei</creatorcontrib><creatorcontrib>He, Ningjia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Dan</au><au>Meng, Shuai</au><au>Xiang, Zhonghuai</au><au>Yang, Guangwei</au><au>He, Ningjia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis )</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-05-27</date><risdate>2019</risdate><volume>20</volume><issue>10</issue><spage>2602</spage><pages>2602-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The aim of this study was to determine how the mulberry (
) polyphenol oxidase 1 gene (
) is regulated during plant stress responses by exploring the interaction between its promoter region and regulatory transcription factors. First, we analyzed the
-acting elements in the
promoter. Then, we used the
promoter region [(1268 bp, including an MYB3R-binding
-element (MSA)] as a probe to capture proteins in DNA pull-down assays. These analyses revealed that the MYB3R1 transcription factor in
(encoded by
) binds to the
promoter region. We further explored the interaction between the
promoter and MYB3R1 with the dual luciferase reporter, yeast one-hybrid, and chromatin immunoprecipitation assays. These analyses verified that MnMYB3R1 binds to the MSA in the
promoter region. The overexpression of
in tobacco upregulated the expression of the tobacco
gene. This observation as well as the quantitative real-time PCR results implied that
and
are involved in the abscisic acid-responsive stress response pathway.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31137877</pmid><doi>10.3390/ijms20102602</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2019-05, Vol.20 (10), p.2602 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Abiotic stress Anthocyanins Barriers Biotin Cell cycle Cell death Cell walls Crosslinking Deoxyribonucleic acid DNA Genes Hydrazine Hydrazines Hydrogen peroxide Hypotheses Mass spectrometry Medical research Melanin Morus notabilis Pathogens Phase transitions Phenolic compounds Phylogenetics Plant growth Plant resistance Polyacrylamide Polymers Polyphenol oxidase Polyphenols PPOs Proteins Quinones Ruthenium Scientific imaging Tobacco Transcription factors Tyramine Tyrosinase Tyrosine Yeast |
| title | An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry ( Morus notabilis ) |
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