Genetic control of cellular morphogenesis in Müller glia
Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell‐specif...
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| Veröffentlicht in: | Glia 2019-07, Vol.67 (7), p.1401-1411 |
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| creator | Charlton‐Perkins, Mark Almeida, Alexandra D. MacDonald, Ryan B. Harris, William A. |
| description | Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell‐specific morphology remain largely unexplored. To identify the genes involved in the morphogenesis of the principal glial cell type in the vertebrate retina, the Müller glia (MG), we used genomic and CRISPR based strategies in zebrafish (Danio rerio). We identified 41 genes involved in various aspects of MG cell morphogenesis and revealed a striking concordance between the sequential steps of anatomical feature addition and the expression of cohorts of functionally related genes that regulate these steps. We noted that the many of the genes preferentially expressed in zebrafish MG showed conservation in glia across species suggesting evolutionarily conserved glial developmental pathways.
Main Points
Müller Glia morphological features are added at distinct stages.
Unique genes are enriched at each stage of Müller Glia morphogenesis.
Müller Glia morphological features arise from distinct gene expression patterns.
Müller Glia morphogenesis is controlled by conserved genetic programs. |
| doi_str_mv | 10.1002/glia.23615 |
| format | Article |
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Main Points
Müller Glia morphological features are added at distinct stages.
Unique genes are enriched at each stage of Müller Glia morphogenesis.
Müller Glia morphological features arise from distinct gene expression patterns.
Müller Glia morphogenesis is controlled by conserved genetic programs.</description><identifier>ISSN: 0894-1491</identifier><identifier>EISSN: 1098-1136</identifier><identifier>DOI: 10.1002/glia.23615</identifier><identifier>PMID: 30924555</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Animals ; Animals, Genetically Modified ; Cell Differentiation - physiology ; Cell size ; Central nervous system ; Clustered Regularly Interspaced Short Palindromic Repeats - physiology ; CRISPR ; Cytology ; Danio rerio ; Ependymoglial Cells - physiology ; Gene expression ; Gene Expression Profiling - methods ; Genes ; Genetic control ; Morphogenesis ; Morphogenesis - physiology ; Morphology ; Müller glia ; Neurogenesis - physiology ; Neuroglia - physiology ; Neuronal-glial interactions ; Retina ; transcriptome ; Transcriptome - physiology ; Vertebrates ; Wildlife conservation ; Zebrafish</subject><ispartof>Glia, 2019-07, Vol.67 (7), p.1401-1411</ispartof><rights>2019 The Authors. published by Wiley Periodicals, Inc.</rights><rights>2019 The Authors. Glia published by Wiley Periodicals, Inc.</rights><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4485-eda0861b50a300ea58c63e3447eb08a75e29487c6c5af040ad5ddd3e1539c3133</citedby><cites>FETCH-LOGICAL-c4485-eda0861b50a300ea58c63e3447eb08a75e29487c6c5af040ad5ddd3e1539c3133</cites><orcidid>0000-0003-4194-8925 ; 0000-0002-9995-8096</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fglia.23615$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fglia.23615$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30924555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Charlton‐Perkins, Mark</creatorcontrib><creatorcontrib>Almeida, Alexandra D.</creatorcontrib><creatorcontrib>MacDonald, Ryan B.</creatorcontrib><creatorcontrib>Harris, William A.</creatorcontrib><title>Genetic control of cellular morphogenesis in Müller glia</title><title>Glia</title><addtitle>Glia</addtitle><description>Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell‐specific morphology remain largely unexplored. To identify the genes involved in the morphogenesis of the principal glial cell type in the vertebrate retina, the Müller glia (MG), we used genomic and CRISPR based strategies in zebrafish (Danio rerio). We identified 41 genes involved in various aspects of MG cell morphogenesis and revealed a striking concordance between the sequential steps of anatomical feature addition and the expression of cohorts of functionally related genes that regulate these steps. We noted that the many of the genes preferentially expressed in zebrafish MG showed conservation in glia across species suggesting evolutionarily conserved glial developmental pathways.
Main Points
Müller Glia morphological features are added at distinct stages.
Unique genes are enriched at each stage of Müller Glia morphogenesis.
Müller Glia morphological features arise from distinct gene expression patterns.
Müller Glia morphogenesis is controlled by conserved genetic programs.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Cell Differentiation - physiology</subject><subject>Cell size</subject><subject>Central nervous system</subject><subject>Clustered Regularly Interspaced Short Palindromic Repeats - physiology</subject><subject>CRISPR</subject><subject>Cytology</subject><subject>Danio rerio</subject><subject>Ependymoglial Cells - physiology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Genes</subject><subject>Genetic control</subject><subject>Morphogenesis</subject><subject>Morphogenesis - physiology</subject><subject>Morphology</subject><subject>Müller glia</subject><subject>Neurogenesis - physiology</subject><subject>Neuroglia - physiology</subject><subject>Neuronal-glial interactions</subject><subject>Retina</subject><subject>transcriptome</subject><subject>Transcriptome - physiology</subject><subject>Vertebrates</subject><subject>Wildlife conservation</subject><subject>Zebrafish</subject><issn>0894-1491</issn><issn>1098-1136</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp9kc1qGzEURkVpqB23mzxAGOgmBCa5-psZbQrGNI7BIZt2LWTNtSMjj1zJk5B3yy4vVjlOQttFV0LocO539RFyQuGCArDLlXfmgvGKyg9kSEE1JaW8-kiG0ChRUqHogByntAag-VJ_IgMOigkp5ZCoKXa4c7awodvF4IuwLCx633sTi02I27uwykRyqXBdcfP85D3GYj_xMzlaGp_wy-s5Ij-vvv-YXJfz2-lsMp6XVohGltgaaCq6kGA4ABrZ2IojF6LGBTSmlsiUaGpbWWmWIMC0sm1bjlRyZTnlfES-HbzbfrHB1mLOabzeRrcx8VEH4_TfL52706twrytZ5TE0C85eBTH86jHt9Mal_Y6mw9AnzRhAXTMuWEa__oOuQx-7vF6mmFBK1KzO1PmBsjGkFHH5HoaC3jei9_-jXxrJ8Omf8d_RtwoyQA_Ag_P4-B-Vns5n44P0N6iRlkM</recordid><startdate>201907</startdate><enddate>201907</enddate><creator>Charlton‐Perkins, Mark</creator><creator>Almeida, Alexandra D.</creator><creator>MacDonald, Ryan B.</creator><creator>Harris, William A.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4194-8925</orcidid><orcidid>https://orcid.org/0000-0002-9995-8096</orcidid></search><sort><creationdate>201907</creationdate><title>Genetic control of cellular morphogenesis in Müller glia</title><author>Charlton‐Perkins, Mark ; Almeida, Alexandra D. ; MacDonald, Ryan B. ; Harris, William A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4485-eda0861b50a300ea58c63e3447eb08a75e29487c6c5af040ad5ddd3e1539c3133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Cell Differentiation - physiology</topic><topic>Cell size</topic><topic>Central nervous system</topic><topic>Clustered Regularly Interspaced Short Palindromic Repeats - physiology</topic><topic>CRISPR</topic><topic>Cytology</topic><topic>Danio rerio</topic><topic>Ependymoglial Cells - physiology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Genes</topic><topic>Genetic control</topic><topic>Morphogenesis</topic><topic>Morphogenesis - physiology</topic><topic>Morphology</topic><topic>Müller glia</topic><topic>Neurogenesis - physiology</topic><topic>Neuroglia - physiology</topic><topic>Neuronal-glial interactions</topic><topic>Retina</topic><topic>transcriptome</topic><topic>Transcriptome - physiology</topic><topic>Vertebrates</topic><topic>Wildlife conservation</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Charlton‐Perkins, Mark</creatorcontrib><creatorcontrib>Almeida, Alexandra D.</creatorcontrib><creatorcontrib>MacDonald, Ryan B.</creatorcontrib><creatorcontrib>Harris, William A.</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Glia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Charlton‐Perkins, Mark</au><au>Almeida, Alexandra D.</au><au>MacDonald, Ryan B.</au><au>Harris, William A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic control of cellular morphogenesis in Müller glia</atitle><jtitle>Glia</jtitle><addtitle>Glia</addtitle><date>2019-07</date><risdate>2019</risdate><volume>67</volume><issue>7</issue><spage>1401</spage><epage>1411</epage><pages>1401-1411</pages><issn>0894-1491</issn><eissn>1098-1136</eissn><abstract>Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell‐specific morphology remain largely unexplored. To identify the genes involved in the morphogenesis of the principal glial cell type in the vertebrate retina, the Müller glia (MG), we used genomic and CRISPR based strategies in zebrafish (Danio rerio). We identified 41 genes involved in various aspects of MG cell morphogenesis and revealed a striking concordance between the sequential steps of anatomical feature addition and the expression of cohorts of functionally related genes that regulate these steps. We noted that the many of the genes preferentially expressed in zebrafish MG showed conservation in glia across species suggesting evolutionarily conserved glial developmental pathways.
Main Points
Müller Glia morphological features are added at distinct stages.
Unique genes are enriched at each stage of Müller Glia morphogenesis.
Müller Glia morphological features arise from distinct gene expression patterns.
Müller Glia morphogenesis is controlled by conserved genetic programs.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30924555</pmid><doi>10.1002/glia.23615</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4194-8925</orcidid><orcidid>https://orcid.org/0000-0002-9995-8096</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Glia, 2019-07, Vol.67 (7), p.1401-1411 |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Animals, Genetically Modified Cell Differentiation - physiology Cell size Central nervous system Clustered Regularly Interspaced Short Palindromic Repeats - physiology CRISPR Cytology Danio rerio Ependymoglial Cells - physiology Gene expression Gene Expression Profiling - methods Genes Genetic control Morphogenesis Morphogenesis - physiology Morphology Müller glia Neurogenesis - physiology Neuroglia - physiology Neuronal-glial interactions Retina transcriptome Transcriptome - physiology Vertebrates Wildlife conservation Zebrafish |
| title | Genetic control of cellular morphogenesis in Müller glia |
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