A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria
Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we com...
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| Veröffentlicht in: | Cell and tissue banking 2019-06, Vol.20 (2), p.173-182 |
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| creator | Liu, Baoyan Lu, Yanqin Wang, Yong Ge, Luna Zhai, Naixiang Han, Jinxiang |
| description | Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests. |
| doi_str_mv | 10.1007/s10561-019-09751-0 |
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Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-019-09751-0</identifier><identifier>PMID: 30887273</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Calvaria ; Cell Adhesion - physiology ; Cell Biology ; Cell growth ; Cell Proliferation - physiology ; Cell Separation - methods ; Enzymes ; Experiments ; Feasibility studies ; Fibroblasts ; Full Length Paper ; Gene expression ; Laboratory animals ; Life Sciences ; Medical research ; Mice ; Mineralization ; Neonates ; Osteoblasts ; Osteoblasts - cytology ; Phosphatase ; Purification ; Rats ; Skull - cytology ; Transplant Surgery ; Transplants & implants</subject><ispartof>Cell and tissue banking, 2019-06, Vol.20 (2), p.173-182</ispartof><rights>The Author(s) 2019</rights><rights>Cell and Tissue Banking is a copyright of Springer, (2019). All Rights Reserved. © 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calvaria</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Biology</subject><subject>Cell growth</subject><subject>Cell Proliferation - physiology</subject><subject>Cell Separation - methods</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Feasibility studies</subject><subject>Fibroblasts</subject><subject>Full Length Paper</subject><subject>Gene expression</subject><subject>Laboratory animals</subject><subject>Life Sciences</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mineralization</subject><subject>Neonates</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Phosphatase</subject><subject>Purification</subject><subject>Rats</subject><subject>Skull - cytology</subject><subject>Transplant Surgery</subject><subject>Transplants & implants</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9vFiEQxomxsbX6BTwYEi-9rMKywHIxaZr6J2nipT0Tlh1amt3lFXjfRC9-dafdt6168MTA_OZhnhlC3nD2njOmPxTOpOIN46ZhRkuMnpEjLrVoVM-75xiL3jRGCHFIXpZyy1jLdCtekEPB-l63WhyRX6d0k1NNPk00pExjSZOrMS3ULSONIyw1huifnnyaNy7jfQfU32DkK-T4cwVSQLU4u_yDplIhDZMrtdCQ00zntC1wL4HV1Ltp53J0r8hBcFOB1_vzmFx9Or88-9JcfPv89ez0ovGyY7URWioFUunRQxjYyDsOAs0MahBhGN3Qe21CC2rk0IFwTPnejJ02osXxaC-OycdVd7MdZkCVpWY32X23Nrlo_84s8cZep51VEmcsDQqc7AVy-r6FUu0ci4dpcgugM9ty0_FO9oYj-u4f9DZt84L27ijRtcwwhVS7Uj6nUjKEx2Y4s3f7tet-LRqw9_u1DIve_mnjseRhoQiIFSiYWq4hP_39H9nfBtu0Ww</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Liu, Baoyan</creator><creator>Lu, Yanqin</creator><creator>Wang, Yong</creator><creator>Ge, Luna</creator><creator>Zhai, Naixiang</creator><creator>Han, Jinxiang</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190601</creationdate><title>A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria</title><author>Liu, Baoyan ; Lu, Yanqin ; Wang, Yong ; Ge, Luna ; Zhai, Naixiang ; Han, Jinxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-37566e567dcefb0d141e3072b6b3fbdab8c79f2e6d1e4e3a06c89d479320197c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Calvaria</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Biology</topic><topic>Cell growth</topic><topic>Cell Proliferation - physiology</topic><topic>Cell Separation - methods</topic><topic>Enzymes</topic><topic>Experiments</topic><topic>Feasibility studies</topic><topic>Fibroblasts</topic><topic>Full Length Paper</topic><topic>Gene expression</topic><topic>Laboratory animals</topic><topic>Life Sciences</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mineralization</topic><topic>Neonates</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Phosphatase</topic><topic>Purification</topic><topic>Rats</topic><topic>Skull - cytology</topic><topic>Transplant Surgery</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Baoyan</creatorcontrib><creatorcontrib>Lu, Yanqin</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Ge, Luna</creatorcontrib><creatorcontrib>Zhai, Naixiang</creatorcontrib><creatorcontrib>Han, Jinxiang</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Baoyan</au><au>Lu, Yanqin</au><au>Wang, Yong</au><au>Ge, Luna</au><au>Zhai, Naixiang</au><au>Han, Jinxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>20</volume><issue>2</issue><spage>173</spage><epage>182</epage><pages>173-182</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30887273</pmid><doi>10.1007/s10561-019-09751-0</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biomedical and Life Sciences Biomedicine Calvaria Cell Adhesion - physiology Cell Biology Cell growth Cell Proliferation - physiology Cell Separation - methods Enzymes Experiments Feasibility studies Fibroblasts Full Length Paper Gene expression Laboratory animals Life Sciences Medical research Mice Mineralization Neonates Osteoblasts Osteoblasts - cytology Phosphatase Purification Rats Skull - cytology Transplant Surgery Transplants & implants |
| title | A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria |
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