Comparative transcriptome profiling of multi-ovary wheat under heterogeneous cytoplasm suppression
DUOII is a multi-ovary wheat line with two or three pistils and three stamens in each floret. The multi-ovary trait of DUOII is controlled by a dominant gene, whose expression can be suppressed by the heterogeneous cytoplasm of TeZhiI (TZI), a line with the nucleus of common wheat and the cytoplasm...
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| description | DUOII is a multi-ovary wheat line with two or three pistils and three stamens in each floret. The multi-ovary trait of DUOII is controlled by a dominant gene, whose expression can be suppressed by the heterogeneous cytoplasm of TeZhiI (TZI), a line with the nucleus of common wheat and the cytoplasm of
Aegilops
. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. Observing the developmental process, we found that the critical stage of additional pistil primordium development was when the young spikes were 2–6 mm long. To elucidate the molecular mechanisms that are responsible for the heterogeneous cytoplasmic suppression of the multi-ovary gene, we RNA-sequenced the entire transcriptome of 2–6 mm long young spikes obtained from the reciprocal crosses between DUOII and TZI. A total of 600 differentially expressed genes (DEGs) was identified. Functional annotation of these DEGs showed that the heterogeneous cytoplasmic suppression of additional pistil development mainly involved four pathways, i.e., chloroplast metabolism, DNA replication and repair, hormone signal transduction, and trehalose-6-phosphate in the primordium development stage, which cooperated to modulate the multi-ovary gene expression under heterogeneous cytoplasmic suppression. |
| doi_str_mv | 10.1038/s41598-019-43277-5 |
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Aegilops
. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. Observing the developmental process, we found that the critical stage of additional pistil primordium development was when the young spikes were 2–6 mm long. To elucidate the molecular mechanisms that are responsible for the heterogeneous cytoplasmic suppression of the multi-ovary gene, we RNA-sequenced the entire transcriptome of 2–6 mm long young spikes obtained from the reciprocal crosses between DUOII and TZI. A total of 600 differentially expressed genes (DEGs) was identified. Functional annotation of these DEGs showed that the heterogeneous cytoplasmic suppression of additional pistil development mainly involved four pathways, i.e., chloroplast metabolism, DNA replication and repair, hormone signal transduction, and trehalose-6-phosphate in the primordium development stage, which cooperated to modulate the multi-ovary gene expression under heterogeneous cytoplasmic suppression.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-43277-5</identifier><identifier>PMID: 31165748</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/77 ; 631/449/2491 ; 631/449/2653 ; Chloroplast DNA ; Chloroplasts ; Chloroplasts - metabolism ; Cytoplasm ; Cytoplasm - metabolism ; Developmental stages ; DNA biosynthesis ; DNA Repair ; DNA Replication ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Humanities and Social Sciences ; Microscopy, Electron, Scanning ; Molecular modelling ; multidisciplinary ; Pistils ; Plant Proteins - genetics ; Ribonucleic acid ; RNA ; RNA-Seq ; Science ; Science (multidisciplinary) ; Signal Transduction ; Stamens ; Sugar Phosphates - metabolism ; Transcriptome ; Transduction ; Trehalose ; Trehalose - analogs & derivatives ; Trehalose - metabolism ; Trehalose-6-phosphate ; Triticum - genetics ; Triticum - physiology ; Wheat</subject><ispartof>Scientific reports, 2019-06, Vol.9 (1), p.8301-8301, Article 8301</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-f44427f0c33477b87f4aab41678d58481597495f982de67a95882f4e9f12c0483</citedby><cites>FETCH-LOGICAL-c511t-f44427f0c33477b87f4aab41678d58481597495f982de67a95882f4e9f12c0483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549160/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6549160/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31165748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Jialin</creatorcontrib><creatorcontrib>Zhang, Gaisheng</creatorcontrib><creatorcontrib>Song, Yulong</creatorcontrib><creatorcontrib>Ma, Shoucai</creatorcontrib><creatorcontrib>Niu, Na</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><title>Comparative transcriptome profiling of multi-ovary wheat under heterogeneous cytoplasm suppression</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>DUOII is a multi-ovary wheat line with two or three pistils and three stamens in each floret. The multi-ovary trait of DUOII is controlled by a dominant gene, whose expression can be suppressed by the heterogeneous cytoplasm of TeZhiI (TZI), a line with the nucleus of common wheat and the cytoplasm of
Aegilops
. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. Observing the developmental process, we found that the critical stage of additional pistil primordium development was when the young spikes were 2–6 mm long. To elucidate the molecular mechanisms that are responsible for the heterogeneous cytoplasmic suppression of the multi-ovary gene, we RNA-sequenced the entire transcriptome of 2–6 mm long young spikes obtained from the reciprocal crosses between DUOII and TZI. A total of 600 differentially expressed genes (DEGs) was identified. Functional annotation of these DEGs showed that the heterogeneous cytoplasmic suppression of additional pistil development mainly involved four pathways, i.e., chloroplast metabolism, DNA replication and repair, hormone signal transduction, and trehalose-6-phosphate in the primordium development stage, which cooperated to modulate the multi-ovary gene expression under heterogeneous cytoplasmic suppression.</description><subject>38/77</subject><subject>631/449/2491</subject><subject>631/449/2653</subject><subject>Chloroplast DNA</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Cytoplasm</subject><subject>Cytoplasm - metabolism</subject><subject>Developmental stages</subject><subject>DNA biosynthesis</subject><subject>DNA Repair</subject><subject>DNA Replication</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Humanities and Social Sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molecular modelling</subject><subject>multidisciplinary</subject><subject>Pistils</subject><subject>Plant Proteins - 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metabolism</topic><topic>Cytoplasm</topic><topic>Cytoplasm - metabolism</topic><topic>Developmental stages</topic><topic>DNA biosynthesis</topic><topic>DNA Repair</topic><topic>DNA Replication</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Humanities and Social Sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molecular modelling</topic><topic>multidisciplinary</topic><topic>Pistils</topic><topic>Plant Proteins - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-Seq</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Signal Transduction</topic><topic>Stamens</topic><topic>Sugar Phosphates - metabolism</topic><topic>Transcriptome</topic><topic>Transduction</topic><topic>Trehalose</topic><topic>Trehalose - analogs & derivatives</topic><topic>Trehalose - metabolism</topic><topic>Trehalose-6-phosphate</topic><topic>Triticum - genetics</topic><topic>Triticum - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Jialin</au><au>Zhang, Gaisheng</au><au>Song, Yulong</au><au>Ma, Shoucai</au><au>Niu, Na</au><au>Wang, Junwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptome profiling of multi-ovary wheat under heterogeneous cytoplasm suppression</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-06-05</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>8301</spage><epage>8301</epage><pages>8301-8301</pages><artnum>8301</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>DUOII is a multi-ovary wheat line with two or three pistils and three stamens in each floret. The multi-ovary trait of DUOII is controlled by a dominant gene, whose expression can be suppressed by the heterogeneous cytoplasm of TeZhiI (TZI), a line with the nucleus of common wheat and the cytoplasm of
Aegilops
. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. Observing the developmental process, we found that the critical stage of additional pistil primordium development was when the young spikes were 2–6 mm long. To elucidate the molecular mechanisms that are responsible for the heterogeneous cytoplasmic suppression of the multi-ovary gene, we RNA-sequenced the entire transcriptome of 2–6 mm long young spikes obtained from the reciprocal crosses between DUOII and TZI. A total of 600 differentially expressed genes (DEGs) was identified. Functional annotation of these DEGs showed that the heterogeneous cytoplasmic suppression of additional pistil development mainly involved four pathways, i.e., chloroplast metabolism, DNA replication and repair, hormone signal transduction, and trehalose-6-phosphate in the primordium development stage, which cooperated to modulate the multi-ovary gene expression under heterogeneous cytoplasmic suppression.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31165748</pmid><doi>10.1038/s41598-019-43277-5</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 38/77 631/449/2491 631/449/2653 Chloroplast DNA Chloroplasts Chloroplasts - metabolism Cytoplasm Cytoplasm - metabolism Developmental stages DNA biosynthesis DNA Repair DNA Replication Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Humanities and Social Sciences Microscopy, Electron, Scanning Molecular modelling multidisciplinary Pistils Plant Proteins - genetics Ribonucleic acid RNA RNA-Seq Science Science (multidisciplinary) Signal Transduction Stamens Sugar Phosphates - metabolism Transcriptome Transduction Trehalose Trehalose - analogs & derivatives Trehalose - metabolism Trehalose-6-phosphate Triticum - genetics Triticum - physiology Wheat |
| title | Comparative transcriptome profiling of multi-ovary wheat under heterogeneous cytoplasm suppression |
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