The in vitro effects of bupivacaine on articular chondrocytes
We have studied the effects of bupivacaine on human and bovine articular chondrocytes in vitro. Time-lapse confocal microscopy of human articular chondrocytes showed > 95% cellular death after exposure to 0.5% bupivacaine for 30 minutes. Human and bovine chondrocytes exposed to 0.25% bupivacaine...
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| Veröffentlicht in: | Journal of bone and joint surgery. British volume 2008-06, Vol.90 (6), p.814-820 |
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| container_title | Journal of bone and joint surgery. British volume |
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| creator | CHU, C. R IZZO, N. J COYLE, C. H PAPAS, N. E LOGAR, A |
| description | We have studied the effects of bupivacaine on human and bovine articular chondrocytes in vitro. Time-lapse confocal microscopy of human articular chondrocytes showed > 95% cellular death after exposure to 0.5% bupivacaine for 30 minutes. Human and bovine chondrocytes exposed to 0.25% bupivacaine had a time-dependent reduction in viability, with longer exposure times resulting in higher cytotoxicity. Cellular death continued even after removal of 0.25% bupivacaine. After exposure to 0.25% bupivacaine for 15 minutes, flow cytometry showed bovine chondrocyte viability to be 41% of saline control after seven days. After exposure to 0.125% bupivacaine for up to 60 minutes, the viability of both bovine and human chondrocytes was similar to that of control groups. These data show that prolonged exposure 0.5% and 0.25% bupivacaine solutions are potentially chondrotoxic. |
| doi_str_mv | 10.1302/0301-620x.90b6.20079 |
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These data show that prolonged exposure 0.5% and 0.25% bupivacaine solutions are potentially chondrotoxic.</description><edition>British volume</edition><identifier>ISSN: 0301-620X</identifier><identifier>ISSN: 2049-4394</identifier><identifier>EISSN: 2044-5377</identifier><identifier>EISSN: 2049-4408</identifier><identifier>DOI: 10.1302/0301-620x.90b6.20079</identifier><identifier>PMID: 18539679</identifier><identifier>CODEN: JBSUAK</identifier><language>eng</language><publisher>London: British Editorial Society of Bone and Joint Surgery</publisher><subject>Alginates ; Anesthetics, Local - pharmacology ; Animals ; Biological and medical sciences ; Bupivacaine - pharmacology ; Cartilage, Articular - cytology ; Cartilage, Articular - drug effects ; Cattle ; Cell Death - drug effects ; Cells, Cultured ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Diseases of the osteoarticular system ; Dose-Response Relationship, Drug ; Humans ; Medical sciences ; Microscopy, Confocal ; Microscopy, Fluorescence ; Orthopedic surgery ; Surgery (general aspects). 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R</creatorcontrib><creatorcontrib>IZZO, N. J</creatorcontrib><creatorcontrib>COYLE, C. H</creatorcontrib><creatorcontrib>PAPAS, N. E</creatorcontrib><creatorcontrib>LOGAR, A</creatorcontrib><title>The in vitro effects of bupivacaine on articular chondrocytes</title><title>Journal of bone and joint surgery. British volume</title><addtitle>J Bone Joint Surg Br</addtitle><description>We have studied the effects of bupivacaine on human and bovine articular chondrocytes in vitro. Time-lapse confocal microscopy of human articular chondrocytes showed > 95% cellular death after exposure to 0.5% bupivacaine for 30 minutes. Human and bovine chondrocytes exposed to 0.25% bupivacaine had a time-dependent reduction in viability, with longer exposure times resulting in higher cytotoxicity. Cellular death continued even after removal of 0.25% bupivacaine. After exposure to 0.25% bupivacaine for 15 minutes, flow cytometry showed bovine chondrocyte viability to be 41% of saline control after seven days. After exposure to 0.125% bupivacaine for up to 60 minutes, the viability of both bovine and human chondrocytes was similar to that of control groups. These data show that prolonged exposure 0.5% and 0.25% bupivacaine solutions are potentially chondrotoxic.</description><subject>Alginates</subject><subject>Anesthetics, Local - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bupivacaine - pharmacology</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cattle</subject><subject>Cell Death - drug effects</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - drug effects</subject><subject>Diseases of the osteoarticular system</subject><subject>Dose-Response Relationship, Drug</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Fluorescence</subject><subject>Orthopedic surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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British volume</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHU, C. R</au><au>IZZO, N. J</au><au>COYLE, C. H</au><au>PAPAS, N. E</au><au>LOGAR, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The in vitro effects of bupivacaine on articular chondrocytes</atitle><jtitle>Journal of bone and joint surgery. British volume</jtitle><addtitle>J Bone Joint Surg Br</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>90</volume><issue>6</issue><spage>814</spage><epage>820</epage><pages>814-820</pages><issn>0301-620X</issn><issn>2049-4394</issn><eissn>2044-5377</eissn><eissn>2049-4408</eissn><coden>JBSUAK</coden><abstract>We have studied the effects of bupivacaine on human and bovine articular chondrocytes in vitro. Time-lapse confocal microscopy of human articular chondrocytes showed > 95% cellular death after exposure to 0.5% bupivacaine for 30 minutes. Human and bovine chondrocytes exposed to 0.25% bupivacaine had a time-dependent reduction in viability, with longer exposure times resulting in higher cytotoxicity. Cellular death continued even after removal of 0.25% bupivacaine. After exposure to 0.25% bupivacaine for 15 minutes, flow cytometry showed bovine chondrocyte viability to be 41% of saline control after seven days. After exposure to 0.125% bupivacaine for up to 60 minutes, the viability of both bovine and human chondrocytes was similar to that of control groups. These data show that prolonged exposure 0.5% and 0.25% bupivacaine solutions are potentially chondrotoxic.</abstract><cop>London</cop><pub>British Editorial Society of Bone and Joint Surgery</pub><pmid>18539679</pmid><doi>10.1302/0301-620x.90b6.20079</doi><tpages>7</tpages><edition>British volume</edition><oa>free_for_read</oa></addata></record> |
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| subjects | Alginates Anesthetics, Local - pharmacology Animals Biological and medical sciences Bupivacaine - pharmacology Cartilage, Articular - cytology Cartilage, Articular - drug effects Cattle Cell Death - drug effects Cells, Cultured Chondrocytes - cytology Chondrocytes - drug effects Diseases of the osteoarticular system Dose-Response Relationship, Drug Humans Medical sciences Microscopy, Confocal Microscopy, Fluorescence Orthopedic surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases |
| title | The in vitro effects of bupivacaine on articular chondrocytes |
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