Microfluidic Collective Cell Migration Assay for Study of Endothelial Cell Proliferation and Migration under Combinations of Oxygen Gradients, Tensions, and Drug Treatments
Proliferation and migration of endothelial cells play an important role in many biological activities, and they can be regulated by various microenvironmental factors. In this paper, a novel microfluidic collective cell migration assay is developed to study endothelial cell migration and proliferati...
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| Veröffentlicht in: | Scientific reports 2019-06, Vol.9 (1), p.8234-8234, Article 8234 |
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| creator | Shih, Hsiu-Chen Lee, Tse-Ang Wu, Hsiao-Mei Ko, Ping-Liang Liao, Wei-Hao Tung, Yi-Chung |
| description | Proliferation and migration of endothelial cells play an important role in many biological activities, and they can be regulated by various microenvironmental factors. In this paper, a novel microfluidic collective cell migration assay is developed to study endothelial cell migration and proliferation under combinations of three oxygen conditions: normoxia, oxygen gradient, and hypoxia and three medium compositions: normal growth medium, the medium with cytochalasin-D for actin polymerization inhibition, and with YC-1 for hypoxia-inducible factor (HIF) inhibition. The microfluidic device designed in the paper allows cell patterns formed with consistent dimensions using laminar flow patterning. In addition, stable oxygen gradients can be generated within the device by a spatially confined chemical reaction method. The device can be operated in conventional cell incubators with minimal chemical reagents and instrumentation for practical applications. The results show directional collective cell migration of the endothelial cells under the oxygen gradients for all the medium compositions. The directional behavior has never been discussed before, and indicates critical roles of oxygen gradients in guiding endothelial cell migration during various biological activities. The developed assay provides a practical yet powerful tool for further i
n vitro
study of endothelial cell behaviors under various physiological microenvironments. |
| doi_str_mv | 10.1038/s41598-019-44594-5 |
| format | Article |
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n vitro
study of endothelial cell behaviors under various physiological microenvironments.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-44594-5</identifier><identifier>PMID: 31160651</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/62 ; 14/63 ; 631/61/32 ; 631/80/84 ; Actin ; Cell adhesion & migration ; Cell migration ; Cell Migration Assays ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Endothelial cells ; Fluorescence ; Human Umbilical Vein Endothelial Cells - cytology ; Human Umbilical Vein Endothelial Cells - drug effects ; Humanities and Social Sciences ; Humans ; Hypoxia ; Hypoxia-inducible factor 1 ; Hypoxia-inducible factors ; Microenvironments ; Microfluidics ; multidisciplinary ; Oxygen ; Oxygen - pharmacology ; Pattern formation ; Pharmaceutical Preparations - metabolism ; Polymerization ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2019-06, Vol.9 (1), p.8234-8234, Article 8234</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-1f4af427b4f53f7ef1c55ba9515d7edb4142c3b7e03a8ac05f9f1f0a8e591ac13</citedby><cites>FETCH-LOGICAL-c577t-1f4af427b4f53f7ef1c55ba9515d7edb4142c3b7e03a8ac05f9f1f0a8e591ac13</cites><orcidid>0000-0002-6170-2992 ; 0000-0002-7027-3069</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546762/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546762/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31160651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shih, Hsiu-Chen</creatorcontrib><creatorcontrib>Lee, Tse-Ang</creatorcontrib><creatorcontrib>Wu, Hsiao-Mei</creatorcontrib><creatorcontrib>Ko, Ping-Liang</creatorcontrib><creatorcontrib>Liao, Wei-Hao</creatorcontrib><creatorcontrib>Tung, Yi-Chung</creatorcontrib><title>Microfluidic Collective Cell Migration Assay for Study of Endothelial Cell Proliferation and Migration under Combinations of Oxygen Gradients, Tensions, and Drug Treatments</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Proliferation and migration of endothelial cells play an important role in many biological activities, and they can be regulated by various microenvironmental factors. In this paper, a novel microfluidic collective cell migration assay is developed to study endothelial cell migration and proliferation under combinations of three oxygen conditions: normoxia, oxygen gradient, and hypoxia and three medium compositions: normal growth medium, the medium with cytochalasin-D for actin polymerization inhibition, and with YC-1 for hypoxia-inducible factor (HIF) inhibition. The microfluidic device designed in the paper allows cell patterns formed with consistent dimensions using laminar flow patterning. In addition, stable oxygen gradients can be generated within the device by a spatially confined chemical reaction method. The device can be operated in conventional cell incubators with minimal chemical reagents and instrumentation for practical applications. The results show directional collective cell migration of the endothelial cells under the oxygen gradients for all the medium compositions. The directional behavior has never been discussed before, and indicates critical roles of oxygen gradients in guiding endothelial cell migration during various biological activities. The developed assay provides a practical yet powerful tool for further i
n vitro
study of endothelial cell behaviors under various physiological microenvironments.</description><subject>13/62</subject><subject>14/63</subject><subject>631/61/32</subject><subject>631/80/84</subject><subject>Actin</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell Migration Assays</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Endothelial cells</subject><subject>Fluorescence</subject><subject>Human Umbilical Vein Endothelial Cells - cytology</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Hypoxia-inducible factor 1</subject><subject>Hypoxia-inducible factors</subject><subject>Microenvironments</subject><subject>Microfluidics</subject><subject>multidisciplinary</subject><subject>Oxygen</subject><subject>Oxygen - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shih, Hsiu-Chen</au><au>Lee, Tse-Ang</au><au>Wu, Hsiao-Mei</au><au>Ko, Ping-Liang</au><au>Liao, Wei-Hao</au><au>Tung, Yi-Chung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic Collective Cell Migration Assay for Study of Endothelial Cell Proliferation and Migration under Combinations of Oxygen Gradients, Tensions, and Drug Treatments</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-06-03</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>8234</spage><epage>8234</epage><pages>8234-8234</pages><artnum>8234</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Proliferation and migration of endothelial cells play an important role in many biological activities, and they can be regulated by various microenvironmental factors. In this paper, a novel microfluidic collective cell migration assay is developed to study endothelial cell migration and proliferation under combinations of three oxygen conditions: normoxia, oxygen gradient, and hypoxia and three medium compositions: normal growth medium, the medium with cytochalasin-D for actin polymerization inhibition, and with YC-1 for hypoxia-inducible factor (HIF) inhibition. The microfluidic device designed in the paper allows cell patterns formed with consistent dimensions using laminar flow patterning. In addition, stable oxygen gradients can be generated within the device by a spatially confined chemical reaction method. The device can be operated in conventional cell incubators with minimal chemical reagents and instrumentation for practical applications. The results show directional collective cell migration of the endothelial cells under the oxygen gradients for all the medium compositions. The directional behavior has never been discussed before, and indicates critical roles of oxygen gradients in guiding endothelial cell migration during various biological activities. The developed assay provides a practical yet powerful tool for further i
n vitro
study of endothelial cell behaviors under various physiological microenvironments.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31160651</pmid><doi>10.1038/s41598-019-44594-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-6170-2992</orcidid><orcidid>https://orcid.org/0000-0002-7027-3069</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/62 14/63 631/61/32 631/80/84 Actin Cell adhesion & migration Cell migration Cell Migration Assays Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Endothelial cells Fluorescence Human Umbilical Vein Endothelial Cells - cytology Human Umbilical Vein Endothelial Cells - drug effects Humanities and Social Sciences Humans Hypoxia Hypoxia-inducible factor 1 Hypoxia-inducible factors Microenvironments Microfluidics multidisciplinary Oxygen Oxygen - pharmacology Pattern formation Pharmaceutical Preparations - metabolism Polymerization Science Science (multidisciplinary) |
| title | Microfluidic Collective Cell Migration Assay for Study of Endothelial Cell Proliferation and Migration under Combinations of Oxygen Gradients, Tensions, and Drug Treatments |
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