Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA
The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Mi...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2019, Vol.81(5), pp.707-711 |
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| creator | KOBAYASHI, Ikuo AKITA, Masaru TAKASU, Masaki TOZAKI, Teruaki KAKOI, Hironaga NAKAMURA, Kotono SENJU, Natsuko MATSUYAMA, Ryota HORII, Yoichiro |
| description | The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time. |
| doi_str_mv | 10.1292/jvms.18-0565 |
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In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.18-0565</identifier><identifier>PMID: 30867351</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>conservation ; genetic characteristics ; Haplotypes ; Heterozygosity ; Horses ; Microsatellites ; Misaki horse ; Mitochondrial DNA ; Phylogeny ; Population genetics ; Wildlife Science</subject><ispartof>Journal of Veterinary Medical Science, 2019, Vol.81(5), pp.707-711</ispartof><rights>2019 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency May 2019</rights><rights>2019 The Japanese Society of Veterinary Science 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c662t-b9dbb713b9696078b25eec66933a3f0c65159e9dff66add92f091b0c8a23d67e3</citedby><cites>FETCH-LOGICAL-c662t-b9dbb713b9696078b25eec66933a3f0c65159e9dff66add92f091b0c8a23d67e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541847/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541847/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30867351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOBAYASHI, Ikuo</creatorcontrib><creatorcontrib>AKITA, Masaru</creatorcontrib><creatorcontrib>TAKASU, Masaki</creatorcontrib><creatorcontrib>TOZAKI, Teruaki</creatorcontrib><creatorcontrib>KAKOI, Hironaga</creatorcontrib><creatorcontrib>NAKAMURA, Kotono</creatorcontrib><creatorcontrib>SENJU, Natsuko</creatorcontrib><creatorcontrib>MATSUYAMA, Ryota</creatorcontrib><creatorcontrib>HORII, Yoichiro</creatorcontrib><title>Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.</description><subject>conservation</subject><subject>genetic characteristics</subject><subject>Haplotypes</subject><subject>Heterozygosity</subject><subject>Horses</subject><subject>Microsatellites</subject><subject>Misaki horse</subject><subject>Mitochondrial DNA</subject><subject>Phylogeny</subject><subject>Population genetics</subject><subject>Wildlife Science</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkc9vFCEYhomxsdvqzbOZxIsHp_JjYOBiUmttm1S96Jkw8E2HdQa2MNuk_30Zd92oFwh8T57w8iL0muAzQhX9sH6Y8hmRNeaCP0Mrwpq2bhumnqMVVkTULeX4GJ3kvMaYkkaoF-iYYSlaxskKhSsIMHtb2cEkY2dIPpdjrmJf9ZDMWH312fzy1RBThlx1JoOrYqg2cXycYtoMPk-_6cnbFLOZYRz9XEgTXLmbox1icMkX0-dv5y_RUW_GDK_2-yn6-eXyx8V1ffv96ubi_La2QtC57pTrupawTgklcCs7ygHKSDFmWI-t4IQrUK7vhTDOKdqXqB220lDmRAvsFH3ceTfbbgJnIcwli94kP5n0qKPx-t9J8IO-iw9a8IbIpi2Cd3tBivdbyLOefLYlmwkQt1lToggTmEtZ0Lf_oeu4TaHE05QyJYjgHBfq_Y5afikn6A-PIVgvReqlSE2kXoos-Ju_AxzgP80V4NMOWOfZ3MEBMKnUN8LOJonmy7K3HoZL2RoCewJe3LRk</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>KOBAYASHI, Ikuo</creator><creator>AKITA, Masaru</creator><creator>TAKASU, Masaki</creator><creator>TOZAKI, Teruaki</creator><creator>KAKOI, Hironaga</creator><creator>NAKAMURA, Kotono</creator><creator>SENJU, Natsuko</creator><creator>MATSUYAMA, Ryota</creator><creator>HORII, Yoichiro</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2019</creationdate><title>Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA</title><author>KOBAYASHI, Ikuo ; AKITA, Masaru ; TAKASU, Masaki ; TOZAKI, Teruaki ; KAKOI, Hironaga ; NAKAMURA, Kotono ; SENJU, Natsuko ; MATSUYAMA, Ryota ; HORII, Yoichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c662t-b9dbb713b9696078b25eec66933a3f0c65159e9dff66add92f091b0c8a23d67e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>conservation</topic><topic>genetic characteristics</topic><topic>Haplotypes</topic><topic>Heterozygosity</topic><topic>Horses</topic><topic>Microsatellites</topic><topic>Misaki horse</topic><topic>Mitochondrial DNA</topic><topic>Phylogeny</topic><topic>Population genetics</topic><topic>Wildlife Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOBAYASHI, Ikuo</creatorcontrib><creatorcontrib>AKITA, Masaru</creatorcontrib><creatorcontrib>TAKASU, Masaki</creatorcontrib><creatorcontrib>TOZAKI, Teruaki</creatorcontrib><creatorcontrib>KAKOI, Hironaga</creatorcontrib><creatorcontrib>NAKAMURA, Kotono</creatorcontrib><creatorcontrib>SENJU, Natsuko</creatorcontrib><creatorcontrib>MATSUYAMA, Ryota</creatorcontrib><creatorcontrib>HORII, Yoichiro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOBAYASHI, Ikuo</au><au>AKITA, Masaru</au><au>TAKASU, Masaki</au><au>TOZAKI, Teruaki</au><au>KAKOI, Hironaga</au><au>NAKAMURA, Kotono</au><au>SENJU, Natsuko</au><au>MATSUYAMA, Ryota</au><au>HORII, Yoichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2019</date><risdate>2019</risdate><volume>81</volume><issue>5</issue><spage>707</spage><epage>711</epage><pages>707-711</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>30867351</pmid><doi>10.1292/jvms.18-0565</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | conservation genetic characteristics Haplotypes Heterozygosity Horses Microsatellites Misaki horse Mitochondrial DNA Phylogeny Population genetics Wildlife Science |
| title | Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA |
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