Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies...
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| Veröffentlicht in: | Translational psychiatry 2019-05, Vol.9 (1), p.153, Article 153 |
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| creator | Sneeboer, Marjolein A. M. Snijders, Gijsje J. L. J. Berdowski, Woutje M. Fernández-Andreu, Alba van Mierlo, Hans C. Berdenis van Berlekom, Amber Litjens, Manja Kahn, René S. Hol, Elly M. de Witte, Lot D. |
| description | Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated. |
| doi_str_mv | 10.1038/s41398-019-0490-x |
| format | Article |
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M. ; Snijders, Gijsje J. L. J. ; Berdowski, Woutje M. ; Fernández-Andreu, Alba ; van Mierlo, Hans C. ; Berdenis van Berlekom, Amber ; Litjens, Manja ; Kahn, René S. ; Hol, Elly M. ; de Witte, Lot D.</creator><creatorcontrib>Sneeboer, Marjolein A. M. ; Snijders, Gijsje J. L. J. ; Berdowski, Woutje M. ; Fernández-Andreu, Alba ; van Mierlo, Hans C. ; Berdenis van Berlekom, Amber ; Litjens, Manja ; Kahn, René S. ; Hol, Elly M. ; de Witte, Lot D. ; Psychiatric Donor Program of the Netherlands Brain Bank (NBB-Psy)</creatorcontrib><description>Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.</description><identifier>ISSN: 2158-3188</identifier><identifier>EISSN: 2158-3188</identifier><identifier>DOI: 10.1038/s41398-019-0490-x</identifier><identifier>PMID: 31127084</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/31 ; 13/51 ; 14 ; 14/63 ; 38 ; 38/77 ; 631/378/340 ; 692/699/476/1333 ; Aged ; Aged, 80 and over ; Autopsy ; Behavioral Sciences ; Biological Psychology ; Biomarkers - metabolism ; Bipolar disorder ; Bipolar Disorder - immunology ; Cerebral Cortex - immunology ; Female ; Humans ; Male ; Medicine ; Medicine & Public Health ; Microglia - immunology ; Middle Aged ; Neurosciences ; Pharmacotherapy ; Psychiatry ; Thalamus - immunology</subject><ispartof>Translational psychiatry, 2019-05, Vol.9 (1), p.153, Article 153</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. 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M.</creatorcontrib><creatorcontrib>Snijders, Gijsje J. L. J.</creatorcontrib><creatorcontrib>Berdowski, Woutje M.</creatorcontrib><creatorcontrib>Fernández-Andreu, Alba</creatorcontrib><creatorcontrib>van Mierlo, Hans C.</creatorcontrib><creatorcontrib>Berdenis van Berlekom, Amber</creatorcontrib><creatorcontrib>Litjens, Manja</creatorcontrib><creatorcontrib>Kahn, René S.</creatorcontrib><creatorcontrib>Hol, Elly M.</creatorcontrib><creatorcontrib>de Witte, Lot D.</creatorcontrib><creatorcontrib>Psychiatric Donor Program of the Netherlands Brain Bank (NBB-Psy)</creatorcontrib><title>Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated</title><title>Translational psychiatry</title><addtitle>Transl Psychiatry</addtitle><addtitle>Transl Psychiatry</addtitle><description>Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. 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M.</au><au>Snijders, Gijsje J. L. J.</au><au>Berdowski, Woutje M.</au><au>Fernández-Andreu, Alba</au><au>van Mierlo, Hans C.</au><au>Berdenis van Berlekom, Amber</au><au>Litjens, Manja</au><au>Kahn, René S.</au><au>Hol, Elly M.</au><au>de Witte, Lot D.</au><aucorp>Psychiatric Donor Program of the Netherlands Brain Bank (NBB-Psy)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated</atitle><jtitle>Translational psychiatry</jtitle><stitle>Transl Psychiatry</stitle><addtitle>Transl Psychiatry</addtitle><date>2019-05-24</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>153</spage><pages>153-</pages><artnum>153</artnum><issn>2158-3188</issn><eissn>2158-3188</eissn><abstract>Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31127084</pmid><doi>10.1038/s41398-019-0490-x</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/31 13/51 14 14/63 38 38/77 631/378/340 692/699/476/1333 Aged Aged, 80 and over Autopsy Behavioral Sciences Biological Psychology Biomarkers - metabolism Bipolar disorder Bipolar Disorder - immunology Cerebral Cortex - immunology Female Humans Male Medicine Medicine & Public Health Microglia - immunology Middle Aged Neurosciences Pharmacotherapy Psychiatry Thalamus - immunology |
| title | Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated |
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