High-resolution phase-contrast imaging of biological specimens using a stable betatron X-ray source in the multiple-exposure mode
Phase-contrast imaging using X-ray sources with high spatial coherence is an emerging tool in biology and material science. Much of this research is being done using large synchrotron facilities or relatively low-flux microfocus X-ray tubes. An alternative high-flux, ultra-short and high-spatial-coh...
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| Veröffentlicht in: | Scientific reports 2019-05, Vol.9 (1), p.7796-7796, Article 7796 |
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| creator | Guo, Bo Zhang, Xiaohui Zhang, Jie Hua, Jianfei Pai, Chih-Hao Zhang, Chaojie Chu, Hsu-Hsin Mori, Warren Joshi, Chan Wang, Jyhpyng Lu, Wei |
| description | Phase-contrast imaging using X-ray sources with high spatial coherence is an emerging tool in biology and material science. Much of this research is being done using large synchrotron facilities or relatively low-flux microfocus X-ray tubes. An alternative high-flux, ultra-short and high-spatial-coherence table-top X-ray source based on betatron motions of electrons in laser wakefield accelerators has the promise to produce high quality images. In previous phase-contrast imaging studies with betatron sources, single-exposure images with a spatial resolution of 6–70
μ
m were reported by using large-scale laser systems (60–200 TW). Furthermore, images obtained with multiple exposures tended to have a reduced contrast and resolution due to the shot-to-shot fluctuations. In this article, we demonstrate that a highly stable multiple-exposure betatron source, with an effective average source size of 5
μ
m, photon number and pointing jitters of |
| doi_str_mv | 10.1038/s41598-019-42834-2 |
| format | Article |
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μ
m were reported by using large-scale laser systems (60–200 TW). Furthermore, images obtained with multiple exposures tended to have a reduced contrast and resolution due to the shot-to-shot fluctuations. In this article, we demonstrate that a highly stable multiple-exposure betatron source, with an effective average source size of 5
μ
m, photon number and pointing jitters of <5% and spectral fluctuation of <10%, can be obtained by utilizing ionization injection in pure nitrogen plasma using a 30–40 TW laser. Using this source, high quality phase-contrast images of biological specimens with a 5-
μ
m resolution are obtained for the first time. This work shows a way for the application of high resolution phase-contrast imaging with stable betatron sources using modest power, high repetition-rate lasers.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-42834-2</identifier><identifier>PMID: 31127147</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>59 ; 639/766/1960/1137 ; 639/766/400/1106 ; CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS ; Humanities and Social Sciences ; Ionization ; Lasers ; multidisciplinary ; Science ; Science & Technology - Other Topics ; Science (multidisciplinary) ; Spatial discrimination</subject><ispartof>Scientific reports, 2019-05, Vol.9 (1), p.7796-7796, Article 7796</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c567t-7587d536eb3c1306f8af7520a0bd653a4b68a0131170111487a31a9de7d14a653</citedby><cites>FETCH-LOGICAL-c567t-7587d536eb3c1306f8af7520a0bd653a4b68a0131170111487a31a9de7d14a653</cites><orcidid>0000-0002-2265-9661 ; 0000000222659661</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534593/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534593/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31127147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1611033$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Bo</creatorcontrib><creatorcontrib>Zhang, Xiaohui</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Hua, Jianfei</creatorcontrib><creatorcontrib>Pai, Chih-Hao</creatorcontrib><creatorcontrib>Zhang, Chaojie</creatorcontrib><creatorcontrib>Chu, Hsu-Hsin</creatorcontrib><creatorcontrib>Mori, Warren</creatorcontrib><creatorcontrib>Joshi, Chan</creatorcontrib><creatorcontrib>Wang, Jyhpyng</creatorcontrib><creatorcontrib>Lu, Wei</creatorcontrib><creatorcontrib>Univ. of California, Los Angeles, CA (United States)</creatorcontrib><title>High-resolution phase-contrast imaging of biological specimens using a stable betatron X-ray source in the multiple-exposure mode</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Phase-contrast imaging using X-ray sources with high spatial coherence is an emerging tool in biology and material science. Much of this research is being done using large synchrotron facilities or relatively low-flux microfocus X-ray tubes. An alternative high-flux, ultra-short and high-spatial-coherence table-top X-ray source based on betatron motions of electrons in laser wakefield accelerators has the promise to produce high quality images. In previous phase-contrast imaging studies with betatron sources, single-exposure images with a spatial resolution of 6–70
μ
m were reported by using large-scale laser systems (60–200 TW). Furthermore, images obtained with multiple exposures tended to have a reduced contrast and resolution due to the shot-to-shot fluctuations. In this article, we demonstrate that a highly stable multiple-exposure betatron source, with an effective average source size of 5
μ
m, photon number and pointing jitters of <5% and spectral fluctuation of <10%, can be obtained by utilizing ionization injection in pure nitrogen plasma using a 30–40 TW laser. Using this source, high quality phase-contrast images of biological specimens with a 5-
μ
m resolution are obtained for the first time. This work shows a way for the application of high resolution phase-contrast imaging with stable betatron sources using modest power, high repetition-rate lasers.</description><subject>59</subject><subject>639/766/1960/1137</subject><subject>639/766/400/1106</subject><subject>CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS</subject><subject>Humanities and Social Sciences</subject><subject>Ionization</subject><subject>Lasers</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science & Technology - Other Topics</subject><subject>Science (multidisciplinary)</subject><subject>Spatial discrimination</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kj1v1zAQxiMEolXpF2BAFiwsBr8lThYkVEGLVIkFJDbLcS6Jq_zj4HMqOvLNcZpSCgNebPl-fs7P3RXFc87ecCbrt6h42dSU8YYqUUtFxaPiWDBVUiGFePzgfFScIl6xvErRKN48LY4k50JzpY-Lnxd-GGkEDNOafJjJMloE6sKcosVE_MEOfh5I6EnrwxQG7-xEcAHnDzAjWXGLWoLJthOQFpJNMct8o9HeEAxrdED8TNII5LBOyS8TUPixBFxjvgkdPCue9HZCOL3bT4qvHz98Obugl5_PP529v6SurHSiuqx1V8oKWum4ZFVf216XglnWdlUprWqr2jKenWnGOVe1tpLbpgPdcWUzcVK823WXtT1A52BzOJklZovxxgTrzd-R2Y9mCNcmv1VlI7PAy10gYPIGnU_gxlyoGVwyvOK5LRv0-i5LDN9XwGQOHh1Mk50hrGhE7kgGGy0y-uof9CqXa841yJRoGlbXDcuU2CkXA2KE_v7HnJltEsw-CSZPgrmdBLNJv3jo9f7J775nQO4A5tA8QPyT-z-yvwDgW7-o</recordid><startdate>20190524</startdate><enddate>20190524</enddate><creator>Guo, Bo</creator><creator>Zhang, Xiaohui</creator><creator>Zhang, Jie</creator><creator>Hua, Jianfei</creator><creator>Pai, Chih-Hao</creator><creator>Zhang, Chaojie</creator><creator>Chu, Hsu-Hsin</creator><creator>Mori, Warren</creator><creator>Joshi, Chan</creator><creator>Wang, Jyhpyng</creator><creator>Lu, Wei</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>OIOZB</scope><scope>OTOTI</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2265-9661</orcidid><orcidid>https://orcid.org/0000000222659661</orcidid></search><sort><creationdate>20190524</creationdate><title>High-resolution phase-contrast imaging of biological specimens using a stable betatron X-ray source in the multiple-exposure mode</title><author>Guo, Bo ; 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Much of this research is being done using large synchrotron facilities or relatively low-flux microfocus X-ray tubes. An alternative high-flux, ultra-short and high-spatial-coherence table-top X-ray source based on betatron motions of electrons in laser wakefield accelerators has the promise to produce high quality images. In previous phase-contrast imaging studies with betatron sources, single-exposure images with a spatial resolution of 6–70
μ
m were reported by using large-scale laser systems (60–200 TW). Furthermore, images obtained with multiple exposures tended to have a reduced contrast and resolution due to the shot-to-shot fluctuations. In this article, we demonstrate that a highly stable multiple-exposure betatron source, with an effective average source size of 5
μ
m, photon number and pointing jitters of <5% and spectral fluctuation of <10%, can be obtained by utilizing ionization injection in pure nitrogen plasma using a 30–40 TW laser. Using this source, high quality phase-contrast images of biological specimens with a 5-
μ
m resolution are obtained for the first time. This work shows a way for the application of high resolution phase-contrast imaging with stable betatron sources using modest power, high repetition-rate lasers.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31127147</pmid><doi>10.1038/s41598-019-42834-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2265-9661</orcidid><orcidid>https://orcid.org/0000000222659661</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 59 639/766/1960/1137 639/766/400/1106 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS Humanities and Social Sciences Ionization Lasers multidisciplinary Science Science & Technology - Other Topics Science (multidisciplinary) Spatial discrimination |
| title | High-resolution phase-contrast imaging of biological specimens using a stable betatron X-ray source in the multiple-exposure mode |
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