Development of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues
At present, there is a lack of well-validated protocols that allow for the analysis of the mechanical properties of muscle and tendon tissues. Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties in vivo using elastography thereby limitin...
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| creator | Kammoun, Malek Ternifi, Redouane Dupres, Vincent Pouletaut, Philippe Même, Sandra Même, William Szeremeta, Frederic Landoulsi, Jessem Constans, Jean-Marc Lafont, Frank Subramaniam, Malayannan Hawse, John R. Bensamoun, Sabine F. |
| description | At present, there is a lack of well-validated protocols that allow for the analysis of the mechanical properties of muscle and tendon tissues. Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties
in vivo
using elastography thereby limiting the ability to monitor changes in these tissues during disease progression or response to therapy. Therefore, we sought to develop novel protocols for the characterization of mechanical properties in musculotendinous tissues using atomic force microscopy (AFM) and ultrasound elastography. Given that TIEG1 knockout (KO) mice exhibit well characterized defects in the mechanical properties of skeletal muscle and tendon tissue, we have chosen to use this model system in the present study. Using TIEG1 knockout and wild-type mice, we have devised an AFM protocol that does not rely on the use of glue or chemical agents for muscle and tendon fiber immobilization during acquisition of transversal cartographies of elasticity and topography. Additionally, since AFM cannot be employed on live animals, we have also developed an ultrasound elastography protocol using a new linear transducer, SLH20-6 (resolution: 38 µm, footprint: 2.38 cm), to characterize the musculotendinous system
in vivo
. This protocol allows for the identification of changes in muscle and tendon elasticities. Such innovative technological approaches have no equivalent to date, promise to accelerate our understanding of musculotendinous mechanical properties and have numerous research and clinical applications. |
| doi_str_mv | 10.1038/s41598-019-44053-1 |
| format | Article |
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in vivo
using elastography thereby limiting the ability to monitor changes in these tissues during disease progression or response to therapy. Therefore, we sought to develop novel protocols for the characterization of mechanical properties in musculotendinous tissues using atomic force microscopy (AFM) and ultrasound elastography. Given that TIEG1 knockout (KO) mice exhibit well characterized defects in the mechanical properties of skeletal muscle and tendon tissue, we have chosen to use this model system in the present study. Using TIEG1 knockout and wild-type mice, we have devised an AFM protocol that does not rely on the use of glue or chemical agents for muscle and tendon fiber immobilization during acquisition of transversal cartographies of elasticity and topography. Additionally, since AFM cannot be employed on live animals, we have also developed an ultrasound elastography protocol using a new linear transducer, SLH20-6 (resolution: 38 µm, footprint: 2.38 cm), to characterize the musculotendinous system
in vivo
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in vivo
using elastography thereby limiting the ability to monitor changes in these tissues during disease progression or response to therapy. Therefore, we sought to develop novel protocols for the characterization of mechanical properties in musculotendinous tissues using atomic force microscopy (AFM) and ultrasound elastography. Given that TIEG1 knockout (KO) mice exhibit well characterized defects in the mechanical properties of skeletal muscle and tendon tissue, we have chosen to use this model system in the present study. Using TIEG1 knockout and wild-type mice, we have devised an AFM protocol that does not rely on the use of glue or chemical agents for muscle and tendon fiber immobilization during acquisition of transversal cartographies of elasticity and topography. Additionally, since AFM cannot be employed on live animals, we have also developed an ultrasound elastography protocol using a new linear transducer, SLH20-6 (resolution: 38 µm, footprint: 2.38 cm), to characterize the musculotendinous system
in vivo
. This protocol allows for the identification of changes in muscle and tendon elasticities. Such innovative technological approaches have no equivalent to date, promise to accelerate our understanding of musculotendinous mechanical properties and have numerous research and clinical applications.</description><subject>59/57</subject><subject>631/1647/767/2200</subject><subject>639/766/747</subject><subject>Achilles Tendon - physiology</subject><subject>Achilles Tendon - ultrastructure</subject><subject>Acoustics</subject><subject>Animal biology</subject><subject>Animals</subject><subject>Atomic force microscopy</subject><subject>Bioengineering</subject><subject>Biological Physics</subject><subject>biomechanical phenomena/methods</subject><subject>Biomechanics</subject><subject>biomedical engineering/methods</subject><subject>Cellular Biology</subject><subject>Chemical agents</subject><subject>Computer Science</subject><subject>Data Analysis, Statistics and Probability</subject><subject>DNA-Binding Proteins - deficiency</subject><subject>Elastic Modulus</subject><subject>Elasticity Imaging Techniques - 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Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties
in vivo
using elastography thereby limiting the ability to monitor changes in these tissues during disease progression or response to therapy. Therefore, we sought to develop novel protocols for the characterization of mechanical properties in musculotendinous tissues using atomic force microscopy (AFM) and ultrasound elastography. Given that TIEG1 knockout (KO) mice exhibit well characterized defects in the mechanical properties of skeletal muscle and tendon tissue, we have chosen to use this model system in the present study. Using TIEG1 knockout and wild-type mice, we have devised an AFM protocol that does not rely on the use of glue or chemical agents for muscle and tendon fiber immobilization during acquisition of transversal cartographies of elasticity and topography. Additionally, since AFM cannot be employed on live animals, we have also developed an ultrasound elastography protocol using a new linear transducer, SLH20-6 (resolution: 38 µm, footprint: 2.38 cm), to characterize the musculotendinous system
in vivo
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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6531478 |
| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 59/57 631/1647/767/2200 639/766/747 Achilles Tendon - physiology Achilles Tendon - ultrastructure Acoustics Animal biology Animals Atomic force microscopy Bioengineering Biological Physics biomechanical phenomena/methods Biomechanics biomedical engineering/methods Cellular Biology Chemical agents Computer Science Data Analysis, Statistics and Probability DNA-Binding Proteins - deficiency Elastic Modulus Elasticity Imaging Techniques - methods Engineering Sciences Female Humanities and Social Sciences image processing, computer assisted/statistics and numerical data Imaging Immobilization Life Sciences Magnetic Resonance Imaging Mechanical properties Mechanics Medical Imaging Medical Physics Mice Mice, Knockout Micro and nanotechnologies Microelectronics Microscopy, Atomic Force - methods Microscopy, Electron multidisciplinary Muscle Fibers, Skeletal - physiology Muscle Fibers, Skeletal - ultrastructure Muscle, Skeletal - physiology Muscle, Skeletal - ultrastructure Musculoskeletal system Nuclear Experiment Physics Protocol Sarcomeres - physiology Sarcomeres - ultrastructure Science Science (multidisciplinary) Signal and Image Processing Skeletal muscle Tendons - physiology Tendons - ultrastructure Therapeutic applications Tissues Transcription Factors - deficiency Ultrasonic imaging Ultrasound |
| title | Development of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T21%3A14%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20novel%20multiphysical%20approach%20for%20the%20characterization%20of%20mechanical%20properties%20of%20musculotendinous%20tissues&rft.jtitle=Scientific%20reports&rft.au=Kammoun,%20Malek&rft.date=2019-05-22&rft.volume=9&rft.issue=1&rft.spage=7733&rft.epage=7733&rft.pages=7733-7733&rft.artnum=7733&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-44053-1&rft_dat=%3Cproquest_pubme%3E2232105978%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2229271891&rft_id=info:pmid/31118478&rfr_iscdi=true |