TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway

TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway

Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioscience reports 2019-05, Vol.39 (5)
Hauptverfasser: Yu, Zhen, Li, Ye, Wang, Yining, Chen, Yuting, Wu, Mengfan, Wang, Zijue, Song, Minkai, Lu, Feng, Lu, Xiaohe, Dong, Ziqing
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 5
container_start_page
container_title Bioscience reports
container_volume 39
creator Yu, Zhen
Li, Ye
Wang, Yining
Chen, Yuting
Wu, Mengfan
Wang, Zijue
Song, Minkai
Lu, Feng
Lu, Xiaohe
Dong, Ziqing
description Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in the skeletal tissue by real-time PCR. RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (runt-related transcription factor 2), Ostecalcin (OC), and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway at least partially.
doi_str_mv 10.1042/BSR20190350
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6522721</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2213916685</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-e27279575ac3c0b2cec6a9039966b045d9149ac720b8bf33272c19bd2aca12023</originalsourceid><addsrcrecordid>eNpVkd9KHDEUh0NpqVvtlfcll4JMmz-Tmc1NQaVaQRDUXockc2YnspOsSWbFF-mD-CA-U7Ouir06IefLd8L5IbRPyXdKavbj-PqKESoJF-QDmlHR8qqWXHxEM0LruprXDd9BX1K6JYSURv0Z7XBKqOAtnaG_N2en1dMjXkVYg88J5wFwBx7iWmcXfOV8N1nocIRSNzc49NgED7gPcXxmsPZdEYQxZNgKnvsRFhvPFslDDNNiwM4Pzrjs_AJPxt1Nm2NV3mbQKYyAVzoP9_phD33q9TLB15e6i_6c_ro5-V1dXJ6dnxxdVJbPaa6AtayVohXacksMs2AbXTYhZdMYUotO0lpq2zJi5qbnvOCWStMxbTVlhPFd9HPrXU1mhM6WFUS9VKvoRh0fVNBO_d_xblCLsFaNYEVGi-DgRRDD3QQpq9ElC8ul9hCmpFhhJG2auSjo4Ra1MaQUoX8bQ4naJKneJVnob-9_9sa-Rsf_AXSEnmY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2213916685</pqid></control><display><type>article</type><title>TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Yu, Zhen ; Li, Ye ; Wang, Yining ; Chen, Yuting ; Wu, Mengfan ; Wang, Zijue ; Song, Minkai ; Lu, Feng ; Lu, Xiaohe ; Dong, Ziqing</creator><creatorcontrib>Yu, Zhen ; Li, Ye ; Wang, Yining ; Chen, Yuting ; Wu, Mengfan ; Wang, Zijue ; Song, Minkai ; Lu, Feng ; Lu, Xiaohe ; Dong, Ziqing</creatorcontrib><description>Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in the skeletal tissue by real-time PCR. RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (runt-related transcription factor 2), Ostecalcin (OC), and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway at least partially.</description><identifier>ISSN: 0144-8463</identifier><identifier>EISSN: 1573-4935</identifier><identifier>DOI: 10.1042/BSR20190350</identifier><identifier>PMID: 31015371</identifier><language>eng</language><publisher>England: Portland Press Ltd</publisher><ispartof>Bioscience reports, 2019-05, Vol.39 (5)</ispartof><rights>2019 The Author(s).</rights><rights>2019 The Author(s). 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-e27279575ac3c0b2cec6a9039966b045d9149ac720b8bf33272c19bd2aca12023</citedby><cites>FETCH-LOGICAL-c381t-e27279575ac3c0b2cec6a9039966b045d9149ac720b8bf33272c19bd2aca12023</cites><orcidid>0000-0002-0255-3915 ; 0000-0001-7110-1173</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522721/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522721/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31015371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Zhen</creatorcontrib><creatorcontrib>Li, Ye</creatorcontrib><creatorcontrib>Wang, Yining</creatorcontrib><creatorcontrib>Chen, Yuting</creatorcontrib><creatorcontrib>Wu, Mengfan</creatorcontrib><creatorcontrib>Wang, Zijue</creatorcontrib><creatorcontrib>Song, Minkai</creatorcontrib><creatorcontrib>Lu, Feng</creatorcontrib><creatorcontrib>Lu, Xiaohe</creatorcontrib><creatorcontrib>Dong, Ziqing</creatorcontrib><title>TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway</title><title>Bioscience reports</title><addtitle>Biosci Rep</addtitle><description>Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in the skeletal tissue by real-time PCR. RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (runt-related transcription factor 2), Ostecalcin (OC), and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway at least partially.</description><issn>0144-8463</issn><issn>1573-4935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVkd9KHDEUh0NpqVvtlfcll4JMmz-Tmc1NQaVaQRDUXockc2YnspOsSWbFF-mD-CA-U7Ouir06IefLd8L5IbRPyXdKavbj-PqKESoJF-QDmlHR8qqWXHxEM0LruprXDd9BX1K6JYSURv0Z7XBKqOAtnaG_N2en1dMjXkVYg88J5wFwBx7iWmcXfOV8N1nocIRSNzc49NgED7gPcXxmsPZdEYQxZNgKnvsRFhvPFslDDNNiwM4Pzrjs_AJPxt1Nm2NV3mbQKYyAVzoP9_phD33q9TLB15e6i_6c_ro5-V1dXJ6dnxxdVJbPaa6AtayVohXacksMs2AbXTYhZdMYUotO0lpq2zJi5qbnvOCWStMxbTVlhPFd9HPrXU1mhM6WFUS9VKvoRh0fVNBO_d_xblCLsFaNYEVGi-DgRRDD3QQpq9ElC8ul9hCmpFhhJG2auSjo4Ra1MaQUoX8bQ4naJKneJVnob-9_9sa-Rsf_AXSEnmY</recordid><startdate>20190531</startdate><enddate>20190531</enddate><creator>Yu, Zhen</creator><creator>Li, Ye</creator><creator>Wang, Yining</creator><creator>Chen, Yuting</creator><creator>Wu, Mengfan</creator><creator>Wang, Zijue</creator><creator>Song, Minkai</creator><creator>Lu, Feng</creator><creator>Lu, Xiaohe</creator><creator>Dong, Ziqing</creator><general>Portland Press Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0255-3915</orcidid><orcidid>https://orcid.org/0000-0001-7110-1173</orcidid></search><sort><creationdate>20190531</creationdate><title>TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway</title><author>Yu, Zhen ; Li, Ye ; Wang, Yining ; Chen, Yuting ; Wu, Mengfan ; Wang, Zijue ; Song, Minkai ; Lu, Feng ; Lu, Xiaohe ; Dong, Ziqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-e27279575ac3c0b2cec6a9039966b045d9149ac720b8bf33272c19bd2aca12023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Zhen</creatorcontrib><creatorcontrib>Li, Ye</creatorcontrib><creatorcontrib>Wang, Yining</creatorcontrib><creatorcontrib>Chen, Yuting</creatorcontrib><creatorcontrib>Wu, Mengfan</creatorcontrib><creatorcontrib>Wang, Zijue</creatorcontrib><creatorcontrib>Song, Minkai</creatorcontrib><creatorcontrib>Lu, Feng</creatorcontrib><creatorcontrib>Lu, Xiaohe</creatorcontrib><creatorcontrib>Dong, Ziqing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bioscience reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Zhen</au><au>Li, Ye</au><au>Wang, Yining</au><au>Chen, Yuting</au><au>Wu, Mengfan</au><au>Wang, Zijue</au><au>Song, Minkai</au><au>Lu, Feng</au><au>Lu, Xiaohe</au><au>Dong, Ziqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway</atitle><jtitle>Bioscience reports</jtitle><addtitle>Biosci Rep</addtitle><date>2019-05-31</date><risdate>2019</risdate><volume>39</volume><issue>5</issue><issn>0144-8463</issn><eissn>1573-4935</eissn><abstract>Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in the skeletal tissue by real-time PCR. RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (runt-related transcription factor 2), Ostecalcin (OC), and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway at least partially.</abstract><cop>England</cop><pub>Portland Press Ltd</pub><pmid>31015371</pmid><doi>10.1042/BSR20190350</doi><orcidid>https://orcid.org/0000-0002-0255-3915</orcidid><orcidid>https://orcid.org/0000-0001-7110-1173</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0144-8463
ispartof Bioscience reports, 2019-05, Vol.39 (5)
issn 0144-8463
1573-4935
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6522721
source EZB-FREE-00999 freely available EZB journals; PubMed Central
title TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T20%3A04%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TGF-%CE%B2%20prevents%20the%20denervation-induced%20reduction%20of%20bone%20formation%20and%20promotes%20the%20bone%20regeneration%20through%20inhibiting%20ubiquitin-proteasome%20pathway&rft.jtitle=Bioscience%20reports&rft.au=Yu,%20Zhen&rft.date=2019-05-31&rft.volume=39&rft.issue=5&rft.issn=0144-8463&rft.eissn=1573-4935&rft_id=info:doi/10.1042/BSR20190350&rft_dat=%3Cproquest_pubme%3E2213916685%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2213916685&rft_id=info:pmid/31015371&rfr_iscdi=true