Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging
A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential wor...
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| Veröffentlicht in: | Microscopy research and technique 2019-06, Vol.82 (6), p.878-883 |
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| creator | Neu‐Baker, Nicole M. Eastlake, Adrienne C. Brenner, Sara A. |
| description | A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential worker exposure. Current best‐known methods include transmission electron microscopy (TEM) coupled with energy dispersive x‐ray spectroscopy (EDS) for elemental identification. TEM‐EDS is considerably time‐, cost‐, and resource‐intensive, which may prevent timely health and safety recommendations and corrective actions. A rapid screening method is currently being explored using enhanced darkfield microscopy with hyperspectral imaging (EDFM‐HSI). For this approach to be effective, rapid, and easy, sample preparation that is amenable to the analytical technique is needed. Here, we compare the sample preparation steps for mixed cellulose ester (MCE) filter media specified in NIOSH Method 7400—Asbestos and Other Fibers by Phase Contrast Microscopy (PCM)—against a new method, which involves saturation of the filter media with acetone. NIOSH Method 7400 was chosen as a starting point since it is an established technique for preparing transparent MCE filters for optical microscopy. Limitations in this method led to the development and comparison of a new method. The new method was faster, easier, and rendered filters more transparent, resulting in improved visualization and analysis of nanomaterials via EDFM‐HSI. This new method is suitable for a rapid screening protocol due to its speed, ease of use, and the improvement in image acquisition and analysis.
NIOSH Method 7400 was compared to a new method for preparing mixed cellulose ester filters for hyperspectral imaging. The new method of filter saturation with liquid acetone yielded improved imaging data for nanoparticles as compared to Method 7400. |
| doi_str_mv | 10.1002/jemt.23231 |
| format | Article |
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NIOSH Method 7400 was compared to a new method for preparing mixed cellulose ester filters for hyperspectral imaging. The new method of filter saturation with liquid acetone yielded improved imaging data for nanoparticles as compared to Method 7400.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.23231</identifier><identifier>PMID: 30768825</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Acetone ; acetone vaporization ; Asbestos ; Cellulose ; Cellulose esters ; Energy transmission ; engineered nanomaterial ; exposure assessment ; Filter media ; Filters ; Hazard assessment ; Hyperspectral imaging ; Image acquisition ; Image processing ; Microscopy ; Nanomaterials ; Nanoparticles ; Nanotechnology ; Occupational exposure ; Occupational health ; Optical microscopy ; Phase contrast ; phase contrast microscopy ; Risk analysis ; Risk assessment ; Safety ; Sample preparation ; Screening ; Spectroscopy ; Transmission electron microscopy ; Visualization</subject><ispartof>Microscopy research and technique, 2019-06, Vol.82 (6), p.878-883</ispartof><rights>Published 2019. This article is a U.S. Government work and is in the public domain in the USA.</rights><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4481-b3bb0dc3052f8dc449cb1800275d67bb650e999929661bf2269125cc6b7b1d973</citedby><cites>FETCH-LOGICAL-c4481-b3bb0dc3052f8dc449cb1800275d67bb650e999929661bf2269125cc6b7b1d973</cites><orcidid>0000-0002-6820-8954</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.23231$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.23231$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30768825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neu‐Baker, Nicole M.</creatorcontrib><creatorcontrib>Eastlake, Adrienne C.</creatorcontrib><creatorcontrib>Brenner, Sara A.</creatorcontrib><title>Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging</title><title>Microscopy research and technique</title><addtitle>Microsc Res Tech</addtitle><description>A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential worker exposure. Current best‐known methods include transmission electron microscopy (TEM) coupled with energy dispersive x‐ray spectroscopy (EDS) for elemental identification. TEM‐EDS is considerably time‐, cost‐, and resource‐intensive, which may prevent timely health and safety recommendations and corrective actions. A rapid screening method is currently being explored using enhanced darkfield microscopy with hyperspectral imaging (EDFM‐HSI). For this approach to be effective, rapid, and easy, sample preparation that is amenable to the analytical technique is needed. Here, we compare the sample preparation steps for mixed cellulose ester (MCE) filter media specified in NIOSH Method 7400—Asbestos and Other Fibers by Phase Contrast Microscopy (PCM)—against a new method, which involves saturation of the filter media with acetone. NIOSH Method 7400 was chosen as a starting point since it is an established technique for preparing transparent MCE filters for optical microscopy. Limitations in this method led to the development and comparison of a new method. The new method was faster, easier, and rendered filters more transparent, resulting in improved visualization and analysis of nanomaterials via EDFM‐HSI. This new method is suitable for a rapid screening protocol due to its speed, ease of use, and the improvement in image acquisition and analysis.
NIOSH Method 7400 was compared to a new method for preparing mixed cellulose ester filters for hyperspectral imaging. The new method of filter saturation with liquid acetone yielded improved imaging data for nanoparticles as compared to Method 7400.</description><subject>Acetone</subject><subject>acetone vaporization</subject><subject>Asbestos</subject><subject>Cellulose</subject><subject>Cellulose esters</subject><subject>Energy transmission</subject><subject>engineered nanomaterial</subject><subject>exposure assessment</subject><subject>Filter media</subject><subject>Filters</subject><subject>Hazard assessment</subject><subject>Hyperspectral imaging</subject><subject>Image acquisition</subject><subject>Image processing</subject><subject>Microscopy</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Occupational exposure</subject><subject>Occupational health</subject><subject>Optical microscopy</subject><subject>Phase contrast</subject><subject>phase contrast microscopy</subject><subject>Risk analysis</subject><subject>Risk assessment</subject><subject>Safety</subject><subject>Sample preparation</subject><subject>Screening</subject><subject>Spectroscopy</subject><subject>Transmission electron microscopy</subject><subject>Visualization</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kstu1TAQhiMEoqWw4QGQJTYIKcV2TpzjDRKqyk1FLCgSO8uXyTk-OHawk0J4Ih4Tm5QKWOCNrfE3v3_PTFU9JPiUYEyfHWCYTmlDG3KrOiaYd3WO8tvl3PKaE_zpqLqX0gFjQlqyuVsdNbhj2y1tj6sfH-QwOkBjhFFGOdng0QDTPhjUh4iubJqls9_Xi9AjL33I4GT17OQESMtxmiMYVPLst3zQ4NzsQgIEaYKIeuvKNoCxEqkFgd9LrzNoZPzcW3AmJ-oYkg7jgqQ3aL-MENMIeorSITvInfW7-9WdXroED673k-rjy_PLs9f1xftXb85eXNR6s9mSWjVKYaMb3NJ-a3KMa0W2uR5da1inFGsx8LwoZ4yonlLGCW21ZqpTxPCuOamer7rjrLJnDb64EGPMPuIigrTi7xtv92IXrgRrKSa0yQJPrgVi-DLnGojBplIU6SHMSVBKee5Rfj6jj_9BD2GOPn-vUJuGMUyK4NOVKkVKEfobMwSLMgCiDID4NQAZfvSn_Rv0d8czQFbgq3Ww_EdKvD1_d7mK_gQ0pMCV</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Neu‐Baker, Nicole M.</creator><creator>Eastlake, Adrienne C.</creator><creator>Brenner, Sara A.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6820-8954</orcidid></search><sort><creationdate>201906</creationdate><title>Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging</title><author>Neu‐Baker, Nicole M. ; Eastlake, Adrienne C. ; Brenner, Sara A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4481-b3bb0dc3052f8dc449cb1800275d67bb650e999929661bf2269125cc6b7b1d973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetone</topic><topic>acetone vaporization</topic><topic>Asbestos</topic><topic>Cellulose</topic><topic>Cellulose esters</topic><topic>Energy transmission</topic><topic>engineered nanomaterial</topic><topic>exposure assessment</topic><topic>Filter media</topic><topic>Filters</topic><topic>Hazard assessment</topic><topic>Hyperspectral imaging</topic><topic>Image acquisition</topic><topic>Image processing</topic><topic>Microscopy</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Occupational exposure</topic><topic>Occupational health</topic><topic>Optical microscopy</topic><topic>Phase contrast</topic><topic>phase contrast microscopy</topic><topic>Risk analysis</topic><topic>Risk assessment</topic><topic>Safety</topic><topic>Sample preparation</topic><topic>Screening</topic><topic>Spectroscopy</topic><topic>Transmission electron microscopy</topic><topic>Visualization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neu‐Baker, Nicole M.</creatorcontrib><creatorcontrib>Eastlake, Adrienne C.</creatorcontrib><creatorcontrib>Brenner, Sara A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microscopy research and technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neu‐Baker, Nicole M.</au><au>Eastlake, Adrienne C.</au><au>Brenner, Sara A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc Res Tech</addtitle><date>2019-06</date><risdate>2019</risdate><volume>82</volume><issue>6</issue><spage>878</spage><epage>883</epage><pages>878-883</pages><issn>1059-910X</issn><eissn>1097-0029</eissn><abstract>A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential worker exposure. Current best‐known methods include transmission electron microscopy (TEM) coupled with energy dispersive x‐ray spectroscopy (EDS) for elemental identification. TEM‐EDS is considerably time‐, cost‐, and resource‐intensive, which may prevent timely health and safety recommendations and corrective actions. A rapid screening method is currently being explored using enhanced darkfield microscopy with hyperspectral imaging (EDFM‐HSI). For this approach to be effective, rapid, and easy, sample preparation that is amenable to the analytical technique is needed. Here, we compare the sample preparation steps for mixed cellulose ester (MCE) filter media specified in NIOSH Method 7400—Asbestos and Other Fibers by Phase Contrast Microscopy (PCM)—against a new method, which involves saturation of the filter media with acetone. NIOSH Method 7400 was chosen as a starting point since it is an established technique for preparing transparent MCE filters for optical microscopy. Limitations in this method led to the development and comparison of a new method. The new method was faster, easier, and rendered filters more transparent, resulting in improved visualization and analysis of nanomaterials via EDFM‐HSI. This new method is suitable for a rapid screening protocol due to its speed, ease of use, and the improvement in image acquisition and analysis.
NIOSH Method 7400 was compared to a new method for preparing mixed cellulose ester filters for hyperspectral imaging. The new method of filter saturation with liquid acetone yielded improved imaging data for nanoparticles as compared to Method 7400.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30768825</pmid><doi>10.1002/jemt.23231</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6820-8954</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Acetone acetone vaporization Asbestos Cellulose Cellulose esters Energy transmission engineered nanomaterial exposure assessment Filter media Filters Hazard assessment Hyperspectral imaging Image acquisition Image processing Microscopy Nanomaterials Nanoparticles Nanotechnology Occupational exposure Occupational health Optical microscopy Phase contrast phase contrast microscopy Risk analysis Risk assessment Safety Sample preparation Screening Spectroscopy Transmission electron microscopy Visualization |
| title | Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging |
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