An acoustofluidic sputum liquefier
We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min(...
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| Veröffentlicht in: | Lab on a chip 2015-08, Vol.15 (15), p.3125-3131 |
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| creator | Huang, Po-Hsun Ren, Liqiang Nama, Nitesh Li, Sixing Li, Peng Yao, Xianglan Cuento, Rosemarie A Wei, Cheng-Hsin Chen, Yuchao Xie, Yuliang Nawaz, Ahmad Ahsan Alevy, Yael G Holtzman, Michael J McCoy, J Philip Levine, Stewart J Huang, Tony Jun |
| description | We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min(-1). Cell viability and integrity are maintained during the sputum liquefaction process. Our acoustofluidic sputum liquefier can be conveniently integrated with other microfluidic units to enable automated on-chip sputum processing and analysis. |
| doi_str_mv | 10.1039/c5lc00539f |
| format | Article |
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Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min(-1). Cell viability and integrity are maintained during the sputum liquefaction process. Our acoustofluidic sputum liquefier can be conveniently integrated with other microfluidic units to enable automated on-chip sputum processing and analysis.</description><identifier>ISSN: 1473-0197</identifier><identifier>EISSN: 1473-0189</identifier><identifier>DOI: 10.1039/c5lc00539f</identifier><identifier>PMID: 26082346</identifier><language>eng</language><publisher>England</publisher><subject>Cell Survival ; Chips ; Devices ; Eosinophils ; Equipment Design ; Flow Cytometry ; Human ; Humans ; Integrity ; Liquefaction ; Liquefiers ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Microfluidics ; Neutrophils ; Oscillating ; Sonication ; Specimen Handling - instrumentation ; Specimen Handling - methods ; Sputum - cytology ; Sputum - physiology</subject><ispartof>Lab on a chip, 2015-08, Vol.15 (15), p.3125-3131</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-a146da6edf6be6f2658583fc1ba371ecb0c444e225c9d4ec34f1e3b3a4dbcd03</citedby><cites>FETCH-LOGICAL-c411t-a146da6edf6be6f2658583fc1ba371ecb0c444e225c9d4ec34f1e3b3a4dbcd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26082346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Po-Hsun</creatorcontrib><creatorcontrib>Ren, Liqiang</creatorcontrib><creatorcontrib>Nama, Nitesh</creatorcontrib><creatorcontrib>Li, Sixing</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><creatorcontrib>Yao, Xianglan</creatorcontrib><creatorcontrib>Cuento, Rosemarie A</creatorcontrib><creatorcontrib>Wei, Cheng-Hsin</creatorcontrib><creatorcontrib>Chen, Yuchao</creatorcontrib><creatorcontrib>Xie, Yuliang</creatorcontrib><creatorcontrib>Nawaz, Ahmad Ahsan</creatorcontrib><creatorcontrib>Alevy, Yael G</creatorcontrib><creatorcontrib>Holtzman, Michael J</creatorcontrib><creatorcontrib>McCoy, J Philip</creatorcontrib><creatorcontrib>Levine, Stewart J</creatorcontrib><creatorcontrib>Huang, Tony Jun</creatorcontrib><title>An acoustofluidic sputum liquefier</title><title>Lab on a chip</title><addtitle>Lab Chip</addtitle><description>We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min(-1). Cell viability and integrity are maintained during the sputum liquefaction process. Our acoustofluidic sputum liquefier can be conveniently integrated with other microfluidic units to enable automated on-chip sputum processing and analysis.</description><subject>Cell Survival</subject><subject>Chips</subject><subject>Devices</subject><subject>Eosinophils</subject><subject>Equipment Design</subject><subject>Flow Cytometry</subject><subject>Human</subject><subject>Humans</subject><subject>Integrity</subject><subject>Liquefaction</subject><subject>Liquefiers</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidics</subject><subject>Neutrophils</subject><subject>Oscillating</subject><subject>Sonication</subject><subject>Specimen Handling - instrumentation</subject><subject>Specimen Handling - methods</subject><subject>Sputum - cytology</subject><subject>Sputum - physiology</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1Lw0AQhhdRbK1e_AFSPIkQ3dmvZC9CCVaFgpfel81-6MomqdlE8N8brRa9eZqBeXh550HoFPAVYCqvDY8GY06l30NTYDnNMBRyf7fLfIKOUnrBGDgTxSGaEIELQpmYovNFM9emHVLf-jgEG8w8bYZ-qOcxvA7OB9cdowOvY3In33OG1svbdXmfrR7vHsrFKjMMoM80MGG1cNaLyglPBC94Qb2BStMcnKmwYYw5QriRljlDmQdHK6qZrYzFdIZutrGboaqdNa7pOx3Vpgu17t5Vq4P6e2nCs3pq35TgUFApx4CL74CuHaunXtUhGRejbtz4oIKcjpY4wD9QIXMChOd0RC-3qOnalDrnd40Aq0_9quSr8kv_coTPfv-wQ3980w8PeICf</recordid><startdate>20150807</startdate><enddate>20150807</enddate><creator>Huang, Po-Hsun</creator><creator>Ren, Liqiang</creator><creator>Nama, Nitesh</creator><creator>Li, Sixing</creator><creator>Li, Peng</creator><creator>Yao, Xianglan</creator><creator>Cuento, Rosemarie A</creator><creator>Wei, Cheng-Hsin</creator><creator>Chen, Yuchao</creator><creator>Xie, Yuliang</creator><creator>Nawaz, Ahmad Ahsan</creator><creator>Alevy, Yael G</creator><creator>Holtzman, Michael J</creator><creator>McCoy, J Philip</creator><creator>Levine, Stewart J</creator><creator>Huang, Tony Jun</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20150807</creationdate><title>An acoustofluidic sputum liquefier</title><author>Huang, Po-Hsun ; Ren, Liqiang ; Nama, Nitesh ; Li, Sixing ; Li, Peng ; Yao, Xianglan ; Cuento, Rosemarie A ; Wei, Cheng-Hsin ; Chen, Yuchao ; Xie, Yuliang ; Nawaz, Ahmad Ahsan ; Alevy, Yael G ; Holtzman, Michael J ; McCoy, J Philip ; Levine, Stewart J ; Huang, Tony Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-a146da6edf6be6f2658583fc1ba371ecb0c444e225c9d4ec34f1e3b3a4dbcd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell Survival</topic><topic>Chips</topic><topic>Devices</topic><topic>Eosinophils</topic><topic>Equipment Design</topic><topic>Flow Cytometry</topic><topic>Human</topic><topic>Humans</topic><topic>Integrity</topic><topic>Liquefaction</topic><topic>Liquefiers</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidics</topic><topic>Neutrophils</topic><topic>Oscillating</topic><topic>Sonication</topic><topic>Specimen Handling - instrumentation</topic><topic>Specimen Handling - methods</topic><topic>Sputum - cytology</topic><topic>Sputum - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Po-Hsun</creatorcontrib><creatorcontrib>Ren, Liqiang</creatorcontrib><creatorcontrib>Nama, Nitesh</creatorcontrib><creatorcontrib>Li, Sixing</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><creatorcontrib>Yao, Xianglan</creatorcontrib><creatorcontrib>Cuento, Rosemarie A</creatorcontrib><creatorcontrib>Wei, Cheng-Hsin</creatorcontrib><creatorcontrib>Chen, Yuchao</creatorcontrib><creatorcontrib>Xie, Yuliang</creatorcontrib><creatorcontrib>Nawaz, Ahmad Ahsan</creatorcontrib><creatorcontrib>Alevy, Yael G</creatorcontrib><creatorcontrib>Holtzman, Michael J</creatorcontrib><creatorcontrib>McCoy, J Philip</creatorcontrib><creatorcontrib>Levine, Stewart J</creatorcontrib><creatorcontrib>Huang, Tony Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Po-Hsun</au><au>Ren, Liqiang</au><au>Nama, Nitesh</au><au>Li, Sixing</au><au>Li, Peng</au><au>Yao, Xianglan</au><au>Cuento, Rosemarie A</au><au>Wei, Cheng-Hsin</au><au>Chen, Yuchao</au><au>Xie, Yuliang</au><au>Nawaz, Ahmad Ahsan</au><au>Alevy, Yael G</au><au>Holtzman, Michael J</au><au>McCoy, J Philip</au><au>Levine, Stewart J</au><au>Huang, Tony Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An acoustofluidic sputum liquefier</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2015-08-07</date><risdate>2015</risdate><volume>15</volume><issue>15</issue><spage>3125</spage><epage>3131</epage><pages>3125-3131</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. 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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Cell Survival Chips Devices Eosinophils Equipment Design Flow Cytometry Human Humans Integrity Liquefaction Liquefiers Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Neutrophils Oscillating Sonication Specimen Handling - instrumentation Specimen Handling - methods Sputum - cytology Sputum - physiology |
| title | An acoustofluidic sputum liquefier |
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