Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp
The secreted mucilage trails of the diatom Navicula sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on th...
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| Veröffentlicht in: | Scientific reports 2019-05, Vol.9 (1), p.7342, Article 7342 |
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| description | The secreted mucilage trails of the diatom
Navicula
sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of
in situ
force mapping by AFM. The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak. Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom’s adhesion and locomotion. In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length. Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, —CH
2
— and —CH
3
asymmetric and symmetric stretches. This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention. |
| doi_str_mv | 10.1038/s41598-019-43663-z |
| format | Article |
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Navicula
sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of
in situ
force mapping by AFM. The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak. Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom’s adhesion and locomotion. In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length. Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, —CH
2
— and —CH
3
asymmetric and symmetric stretches. This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-43663-z</identifier><identifier>PMID: 31089153</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>639/638/542/967 ; 704/172/169/827 ; Adhesives - metabolism ; Atomic force microscopy ; Biofouling ; Cell Adhesion ; Cell Movement ; Diatoms - cytology ; Diatoms - metabolism ; Humanities and Social Sciences ; Locomotion ; Mechanical properties ; Microscopy ; Motility ; Mucilage ; multidisciplinary ; Navicula ; Polymers ; Polysaccharides ; Polysaccharides - metabolism ; Proteins - metabolism ; Raman spectroscopy ; Scanning electron microscopy ; Science ; Science (multidisciplinary) ; Surface properties ; Trails</subject><ispartof>Scientific reports, 2019-05, Vol.9 (1), p.7342, Article 7342</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-4396bb789eb2ef2be2a9bd3200a80874e5b89f916c5826708479fa1a757e8bbc3</citedby><cites>FETCH-LOGICAL-c577t-4396bb789eb2ef2be2a9bd3200a80874e5b89f916c5826708479fa1a757e8bbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517400/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517400/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31089153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Weng, Ding</creatorcontrib><creatorcontrib>Du, Chuan</creatorcontrib><creatorcontrib>Wang, Jiadao</creatorcontrib><creatorcontrib>Cao, Shan</creatorcontrib><title>Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The secreted mucilage trails of the diatom
Navicula
sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of
in situ
force mapping by AFM. The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak. Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom’s adhesion and locomotion. In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length. Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, —CH
2
— and —CH
3
asymmetric and symmetric stretches. This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.</description><subject>639/638/542/967</subject><subject>704/172/169/827</subject><subject>Adhesives - metabolism</subject><subject>Atomic force microscopy</subject><subject>Biofouling</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Diatoms - cytology</subject><subject>Diatoms - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Locomotion</subject><subject>Mechanical properties</subject><subject>Microscopy</subject><subject>Motility</subject><subject>Mucilage</subject><subject>multidisciplinary</subject><subject>Navicula</subject><subject>Polymers</subject><subject>Polysaccharides</subject><subject>Polysaccharides - metabolism</subject><subject>Proteins - metabolism</subject><subject>Raman spectroscopy</subject><subject>Scanning electron microscopy</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Surface properties</subject><subject>Trails</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UU1LAzEUDKKoaP-ABwl4Xs3HZpNcBCl-QdGDeg7JNttGdjc1yRbqrzdarfViLi_wZubNewPACUbnGFFxEUvMpCgQlkVJq4oW7zvgkKCSFYQSsrv1PwCjGF9RfozIEst9cEAxEhIzegiexr5PwZkhOd9D38AmDDENrY1Q91PYDbVr9czCFLRrI0weprmFnTeudWn1SZg6nXwHH_TS1UOrYVwcg71Gt9GOvusReLm5fh7fFZPH2_vx1aSoGecp25aVMVxIa4htiLFESzOlBCEtkOClZUbIRuKqZoJUHImSy0ZjzRm3wpiaHoHLte5iMJ2d1jZvolu1CK7TYaW8dupvp3dzNfNLVTHMS4SywNm3QPBvg41Jvfoh9NmzIoQwTJFEJKPIGlUHH2OwzWYCRuozC7XOQuUs1FcW6j2TTre9bSg_l88AugbE3OpnNvzO_kf2A7URlnQ</recordid><startdate>20190514</startdate><enddate>20190514</enddate><creator>Chen, Lei</creator><creator>Weng, Ding</creator><creator>Du, Chuan</creator><creator>Wang, Jiadao</creator><creator>Cao, Shan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20190514</creationdate><title>Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp</title><author>Chen, Lei ; Weng, Ding ; Du, Chuan ; Wang, Jiadao ; Cao, Shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-4396bb789eb2ef2be2a9bd3200a80874e5b89f916c5826708479fa1a757e8bbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>639/638/542/967</topic><topic>704/172/169/827</topic><topic>Adhesives - metabolism</topic><topic>Atomic force microscopy</topic><topic>Biofouling</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Diatoms - cytology</topic><topic>Diatoms - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Locomotion</topic><topic>Mechanical properties</topic><topic>Microscopy</topic><topic>Motility</topic><topic>Mucilage</topic><topic>multidisciplinary</topic><topic>Navicula</topic><topic>Polymers</topic><topic>Polysaccharides</topic><topic>Polysaccharides - metabolism</topic><topic>Proteins - metabolism</topic><topic>Raman spectroscopy</topic><topic>Scanning electron microscopy</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Surface properties</topic><topic>Trails</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Weng, Ding</creatorcontrib><creatorcontrib>Du, Chuan</creatorcontrib><creatorcontrib>Wang, Jiadao</creatorcontrib><creatorcontrib>Cao, Shan</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Lei</au><au>Weng, Ding</au><au>Du, Chuan</au><au>Wang, Jiadao</au><au>Cao, Shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-05-14</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>7342</spage><pages>7342-</pages><artnum>7342</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The secreted mucilage trails of the diatom
Navicula
sp. in the process of motility were studied by scanning electron microscopy (SEM), transmission electron microscope (TEM), atomic force microscopy (AFM) and Raman spectra etc. Contrary to previous studies, force measurement was taken directly on the mucilage trails of live cells using the method of
in situ
force mapping by AFM. The retraction force curve presented an increased tip-substrate peak and a small saw-tooth pattern tip-mucilage peak. Especially, same measurements on various substrates with different surface energy revealed that the mucilage trails actually functioned as a medium increasing the adhesive force between the diatom and substrates, which is crucial to diatom’s adhesion and locomotion. In addition, the mechanical properties of mucilage trails were quite different from mucilage strands in the maximum adhesive force and the maximum polymer extension length. Raman spectra indicated the difference in compositions that both of the two kinds of mucilages had proteins and polysaccharide, but the mucilage strands contained some other components with C=O, —CH
2
— and —CH
3
asymmetric and symmetric stretches. This research hammers out more precise information about mucilage trails which would be useful in terms of diatom motility and biofouling prevention.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31089153</pmid><doi>10.1038/s41598-019-43663-z</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 639/638/542/967 704/172/169/827 Adhesives - metabolism Atomic force microscopy Biofouling Cell Adhesion Cell Movement Diatoms - cytology Diatoms - metabolism Humanities and Social Sciences Locomotion Mechanical properties Microscopy Motility Mucilage multidisciplinary Navicula Polymers Polysaccharides Polysaccharides - metabolism Proteins - metabolism Raman spectroscopy Scanning electron microscopy Science Science (multidisciplinary) Surface properties Trails |
| title | Contribution of frustules and mucilage trails to the mobility of diatom Navicula sp |
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