Aminoacyl-tRNA Synthetases and tRNAs for an Expanded Genetic Code: What Makes them Orthogonal?
In the past two decades, tRNA molecules and their corresponding aminoacyl-tRNA synthetases (aaRS) have been extensively used in synthetic biology to genetically encode post-translationally modified and unnatural amino acids. In this review, we briefly examine one fundamental requirement for the succ...
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| Veröffentlicht in: | International journal of molecular sciences 2019-04, Vol.20 (8), p.1929 |
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| creator | Melnikov, Sergey V Söll, Dieter |
| description | In the past two decades, tRNA molecules and their corresponding aminoacyl-tRNA synthetases (aaRS) have been extensively used in synthetic biology to genetically encode post-translationally modified and unnatural amino acids. In this review, we briefly examine one fundamental requirement for the successful application of tRNA/aaRS pairs for expanding the genetic code. This requirement is known as "orthogonality"-the ability of a tRNA and its corresponding aaRS to interact exclusively with each other and avoid cross-reactions with additional types of tRNAs and aaRSs in a given organism. |
| doi_str_mv | 10.3390/ijms20081929 |
| format | Article |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Alanine Alanine-tRNA ligase Amino acids Aminoacylation Bacteria Catalytic activity Codons E coli Evolution Genetic code Genetic engineering Genomes Leucine Morphogenesis Orthogonality Phages Proteins Review Serine Substrate specificity Transplantation tRNA Virulence |
| title | Aminoacyl-tRNA Synthetases and tRNAs for an Expanded Genetic Code: What Makes them Orthogonal? |
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