Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization
Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers incl...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2019-08, Vol.107 (6), p.1792-1805 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Manoukian, Ohan S. Stratton, Scott Arul, Michael R. Moskow, Joshua Sardashti, Naseem Yu, Xiaojun Rudraiah, Swetha Kumbar, Sangamesh G. |
| description | Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal‐like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite‐like structures with cell bodies for ES‐treated and positive control growth factor‐treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3‐tubulin, microtubule‐associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792–1805, 2019. |
| doi_str_mv | 10.1002/jbm.b.34272 |
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Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal‐like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite‐like structures with cell bodies for ES‐treated and positive control growth factor‐treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3‐tubulin, microtubule‐associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792–1805, 2019.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.34272</identifier><identifier>PMID: 30419159</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Acetic acid ; Biomedical materials ; Cell viability ; Cellulose acetate ; Chitosan ; Coating ; Coatings ; Conductivity ; Cytology ; Electrical resistivity ; electrical stimulation ; Electrical stimuli ; Elongated structure ; Growth factors ; Integrated circuits ; Ion currents ; ionic conductivity ; Lignin ; Materials research ; Materials science ; Mechanical properties ; Mesenchyme ; micro–nanofiber ; Morphology ; Nanofibers ; nerve regeneration ; Nestin ; Peripheral nerves ; Phenotypes ; Polyanilines ; Polycaprolactone ; Polymers ; Regeneration ; scaffold ; Stem cells ; Stimulation ; Thin films ; Three dimensional composites ; Tubulin ; Two dimensional composites</subject><ispartof>Journal of biomedical materials research. 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Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal‐like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite‐like structures with cell bodies for ES‐treated and positive control growth factor‐treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3‐tubulin, microtubule‐associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792–1805, 2019.</description><subject>Acetic acid</subject><subject>Biomedical materials</subject><subject>Cell viability</subject><subject>Cellulose acetate</subject><subject>Chitosan</subject><subject>Coating</subject><subject>Coatings</subject><subject>Conductivity</subject><subject>Cytology</subject><subject>Electrical resistivity</subject><subject>electrical stimulation</subject><subject>Electrical stimuli</subject><subject>Elongated structure</subject><subject>Growth factors</subject><subject>Integrated circuits</subject><subject>Ion currents</subject><subject>ionic conductivity</subject><subject>Lignin</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Mechanical properties</subject><subject>Mesenchyme</subject><subject>micro–nanofiber</subject><subject>Morphology</subject><subject>Nanofibers</subject><subject>nerve regeneration</subject><subject>Nestin</subject><subject>Peripheral nerves</subject><subject>Phenotypes</subject><subject>Polyanilines</subject><subject>Polycaprolactone</subject><subject>Polymers</subject><subject>Regeneration</subject><subject>scaffold</subject><subject>Stem cells</subject><subject>Stimulation</subject><subject>Thin films</subject><subject>Three dimensional composites</subject><subject>Tubulin</subject><subject>Two dimensional composites</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU1vEzEQhlcIREvhxB1Z4oKEEjy294sDEq34KCqCA5wt2zubOvKug70bFP4B_5ppUiLgwMmvNY_fmfFbFI-BL4Fz8WJth6VdSiVqcac4hbIUC9U2cPeoa3lSPMh5TXDFS3m_OJFcQQtle1r8_BzDbsDkHfNx9M6EsGMujt3sJr9FksMmZj8hG8xEFGZmxo5hQHdzNYHlyQ9zMBM9J53MhCtPVB8TGzGRRcIVktoTL9nlyLZ-SpG5a5OMm6j1j33pYXGvNyHjo9vzrPj69s2Xi_eLq0_vLi9eXy2cKoVYtLw1TddLB6KWjagUWNdBbxtZ97ypoWosB2GrmtsejWxp017xsmsUcKsqkGfFq4PvZrYDdg5HGjroTfKDSTsdjdd_V0Z_rVdxq6sSgD6WDJ7dGqT4bcY86cFnhyGYEeOctQApagUgOKFP_0HXcU4jraeFKKGtlJI1Uc8PlEsx54T9cRjg-iZiTRFrq_cRE_3kz_mP7O9MCRAH4LsPuPufl_5w_vH84PoLpia1WQ</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Manoukian, Ohan S.</creator><creator>Stratton, Scott</creator><creator>Arul, Michael R.</creator><creator>Moskow, Joshua</creator><creator>Sardashti, Naseem</creator><creator>Yu, Xiaojun</creator><creator>Rudraiah, Swetha</creator><creator>Kumbar, Sangamesh G.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201908</creationdate><title>Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization</title><author>Manoukian, Ohan S. ; 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Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manoukian, Ohan S.</au><au>Stratton, Scott</au><au>Arul, Michael R.</au><au>Moskow, Joshua</au><au>Sardashti, Naseem</au><au>Yu, Xiaojun</au><au>Rudraiah, Swetha</au><au>Kumbar, Sangamesh G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><date>2019-08</date><risdate>2019</risdate><volume>107</volume><issue>6</issue><spage>1792</spage><epage>1805</epage><pages>1792-1805</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal‐like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite‐like structures with cell bodies for ES‐treated and positive control growth factor‐treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3‐tubulin, microtubule‐associated protein 2, and nestin in response to ES. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1792–1805, 2019.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30419159</pmid><doi>10.1002/jbm.b.34272</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetic acid Biomedical materials Cell viability Cellulose acetate Chitosan Coating Coatings Conductivity Cytology Electrical resistivity electrical stimulation Electrical stimuli Elongated structure Growth factors Integrated circuits Ion currents ionic conductivity Lignin Materials research Materials science Mechanical properties Mesenchyme micro–nanofiber Morphology Nanofibers nerve regeneration Nestin Peripheral nerves Phenotypes Polyanilines Polycaprolactone Polymers Regeneration scaffold Stem cells Stimulation Thin films Three dimensional composites Tubulin Two dimensional composites |
| title | Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization |
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