Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes
We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated wi...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2019-05, Vol.9 (1), p.7187-7187, Article 7187 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7187 |
|---|---|
| container_issue | 1 |
| container_start_page | 7187 |
| container_title | Scientific reports |
| container_volume | 9 |
| creator | Busselez, Johan Chichón, Francisco Javier Rodríguez, Maria Josefa Alpízar, Adan Gharbi, Séverine Isabelle Franch, Mònica Melero, Roberto Paradela, Alberto Carrascosa, José L. Carazo, José-Maria |
| description | We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated with centriole disengagement and centrosome separation. The data obtained enhances our understanding of the protein system joining the centrioles, a system comprised of a branched network of fibers linked to an apparently amorphous density that was partially characterized here. A number of proteins were localized to the amorphous density by immunolabeling (C-NAP1, cohesin SMC1, condensin SMC4 and NCAPD2), yet not DNA. In conjuction, these data not only extend our understanding of centrosomes but they will help refine the model that focus on the protein system associated with the centriolar junction. |
| doi_str_mv | 10.1038/s41598-019-43338-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6510768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2231910751</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-af28e2bc36cfc3b33cb5f1bfdce352f9f8615b1c39892bb7ae3edd60971a04783</originalsourceid><addsrcrecordid>eNp9kUtLxDAQx4MoKrpfwIMEvHip5tF0k4sgiy8Q9LCeQ5pOdittsyat0G9v1vV9MJc85jf_zMwfoSNKzijh8jzmVCiZEaqynHMuM7WF9hnJRcY4Y9s_zntoEuMzSUswlVO1i_Y4JdNCSLmPFrMw-uyqAdsH3-G5b_0imNVyxKar8GPwPfi2thHHfqhqiNg7bKFLcPRturrgW1zVzkFIr7XpocIvQwLXEO6XY-vt2EM8RDvONBEmH_sBerq-ms9us_uHm7vZ5X1mBaV9ZhyTwErLC-ssLzm3pXC0dJUFLphTThZUlNRyJRUry6kBDlVVEDWlhuRTyQ_QxUZ3NZQtVO-lmkavQt2aMGpvav070tVLvfCvuhDrmawFTj8Egn8ZIPa6rVMzTWM68EPUjHGqEipoQk_-oM9-CF1qL1GMKZYrWSSKbSibZhYDuK9iKNFrK_XGSp2s1O9WapWSjn-28ZXyaVwC-AaIKdQtIHz__Y_sG9JMrUY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2222924986</pqid></control><display><type>article</type><title>Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Busselez, Johan ; Chichón, Francisco Javier ; Rodríguez, Maria Josefa ; Alpízar, Adan ; Gharbi, Séverine Isabelle ; Franch, Mònica ; Melero, Roberto ; Paradela, Alberto ; Carrascosa, José L. ; Carazo, José-Maria</creator><creatorcontrib>Busselez, Johan ; Chichón, Francisco Javier ; Rodríguez, Maria Josefa ; Alpízar, Adan ; Gharbi, Séverine Isabelle ; Franch, Mònica ; Melero, Roberto ; Paradela, Alberto ; Carrascosa, José L. ; Carazo, José-Maria</creatorcontrib><description>We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated with centriole disengagement and centrosome separation. The data obtained enhances our understanding of the protein system joining the centrioles, a system comprised of a branched network of fibers linked to an apparently amorphous density that was partially characterized here. A number of proteins were localized to the amorphous density by immunolabeling (C-NAP1, cohesin SMC1, condensin SMC4 and NCAPD2), yet not DNA. In conjuction, these data not only extend our understanding of centrosomes but they will help refine the model that focus on the protein system associated with the centriolar junction.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-43338-9</identifier><identifier>PMID: 31076588</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 14/1 ; 14/19 ; 38/79 ; 631/114/2784 ; 631/535/1258/1260 ; 631/80/641 ; 82/58 ; 82/83 ; Animals ; Cell Line ; Centrioles ; Centrosome - metabolism ; Centrosomes ; Cohesin ; Condensin ; Cryoelectron Microscopy ; Electron Microscope Tomography ; Fibers ; Gene Regulatory Networks ; Humanities and Social Sciences ; multidisciplinary ; Proteomics ; Proteomics - methods ; Science ; Science (multidisciplinary) ; Sheep ; Thymocytes ; Thymocytes - cytology ; Thymocytes - metabolism ; Thymus ; Tomography</subject><ispartof>Scientific reports, 2019-05, Vol.9 (1), p.7187-7187, Article 7187</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-af28e2bc36cfc3b33cb5f1bfdce352f9f8615b1c39892bb7ae3edd60971a04783</citedby><cites>FETCH-LOGICAL-c511t-af28e2bc36cfc3b33cb5f1bfdce352f9f8615b1c39892bb7ae3edd60971a04783</cites><orcidid>0000-0003-0788-8447 ; 0000-0002-4078-1265 ; 0000-0003-2766-0327 ; 0000-0001-6837-7056</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510768/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510768/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31076588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Busselez, Johan</creatorcontrib><creatorcontrib>Chichón, Francisco Javier</creatorcontrib><creatorcontrib>Rodríguez, Maria Josefa</creatorcontrib><creatorcontrib>Alpízar, Adan</creatorcontrib><creatorcontrib>Gharbi, Séverine Isabelle</creatorcontrib><creatorcontrib>Franch, Mònica</creatorcontrib><creatorcontrib>Melero, Roberto</creatorcontrib><creatorcontrib>Paradela, Alberto</creatorcontrib><creatorcontrib>Carrascosa, José L.</creatorcontrib><creatorcontrib>Carazo, José-Maria</creatorcontrib><title>Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated with centriole disengagement and centrosome separation. The data obtained enhances our understanding of the protein system joining the centrioles, a system comprised of a branched network of fibers linked to an apparently amorphous density that was partially characterized here. A number of proteins were localized to the amorphous density by immunolabeling (C-NAP1, cohesin SMC1, condensin SMC4 and NCAPD2), yet not DNA. In conjuction, these data not only extend our understanding of centrosomes but they will help refine the model that focus on the protein system associated with the centriolar junction.</description><subject>101/28</subject><subject>14/1</subject><subject>14/19</subject><subject>38/79</subject><subject>631/114/2784</subject><subject>631/535/1258/1260</subject><subject>631/80/641</subject><subject>82/58</subject><subject>82/83</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Centrioles</subject><subject>Centrosome - metabolism</subject><subject>Centrosomes</subject><subject>Cohesin</subject><subject>Condensin</subject><subject>Cryoelectron Microscopy</subject><subject>Electron Microscope Tomography</subject><subject>Fibers</subject><subject>Gene Regulatory Networks</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sheep</subject><subject>Thymocytes</subject><subject>Thymocytes - cytology</subject><subject>Thymocytes - metabolism</subject><subject>Thymus</subject><subject>Tomography</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtLxDAQx4MoKrpfwIMEvHip5tF0k4sgiy8Q9LCeQ5pOdittsyat0G9v1vV9MJc85jf_zMwfoSNKzijh8jzmVCiZEaqynHMuM7WF9hnJRcY4Y9s_zntoEuMzSUswlVO1i_Y4JdNCSLmPFrMw-uyqAdsH3-G5b_0imNVyxKar8GPwPfi2thHHfqhqiNg7bKFLcPRturrgW1zVzkFIr7XpocIvQwLXEO6XY-vt2EM8RDvONBEmH_sBerq-ms9us_uHm7vZ5X1mBaV9ZhyTwErLC-ssLzm3pXC0dJUFLphTThZUlNRyJRUry6kBDlVVEDWlhuRTyQ_QxUZ3NZQtVO-lmkavQt2aMGpvav070tVLvfCvuhDrmawFTj8Egn8ZIPa6rVMzTWM68EPUjHGqEipoQk_-oM9-CF1qL1GMKZYrWSSKbSibZhYDuK9iKNFrK_XGSp2s1O9WapWSjn-28ZXyaVwC-AaIKdQtIHz__Y_sG9JMrUY</recordid><startdate>20190510</startdate><enddate>20190510</enddate><creator>Busselez, Johan</creator><creator>Chichón, Francisco Javier</creator><creator>Rodríguez, Maria Josefa</creator><creator>Alpízar, Adan</creator><creator>Gharbi, Séverine Isabelle</creator><creator>Franch, Mònica</creator><creator>Melero, Roberto</creator><creator>Paradela, Alberto</creator><creator>Carrascosa, José L.</creator><creator>Carazo, José-Maria</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0788-8447</orcidid><orcidid>https://orcid.org/0000-0002-4078-1265</orcidid><orcidid>https://orcid.org/0000-0003-2766-0327</orcidid><orcidid>https://orcid.org/0000-0001-6837-7056</orcidid></search><sort><creationdate>20190510</creationdate><title>Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes</title><author>Busselez, Johan ; Chichón, Francisco Javier ; Rodríguez, Maria Josefa ; Alpízar, Adan ; Gharbi, Séverine Isabelle ; Franch, Mònica ; Melero, Roberto ; Paradela, Alberto ; Carrascosa, José L. ; Carazo, José-Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-af28e2bc36cfc3b33cb5f1bfdce352f9f8615b1c39892bb7ae3edd60971a04783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>101/28</topic><topic>14/1</topic><topic>14/19</topic><topic>38/79</topic><topic>631/114/2784</topic><topic>631/535/1258/1260</topic><topic>631/80/641</topic><topic>82/58</topic><topic>82/83</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Centrioles</topic><topic>Centrosome - metabolism</topic><topic>Centrosomes</topic><topic>Cohesin</topic><topic>Condensin</topic><topic>Cryoelectron Microscopy</topic><topic>Electron Microscope Tomography</topic><topic>Fibers</topic><topic>Gene Regulatory Networks</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sheep</topic><topic>Thymocytes</topic><topic>Thymocytes - cytology</topic><topic>Thymocytes - metabolism</topic><topic>Thymus</topic><topic>Tomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Busselez, Johan</creatorcontrib><creatorcontrib>Chichón, Francisco Javier</creatorcontrib><creatorcontrib>Rodríguez, Maria Josefa</creatorcontrib><creatorcontrib>Alpízar, Adan</creatorcontrib><creatorcontrib>Gharbi, Séverine Isabelle</creatorcontrib><creatorcontrib>Franch, Mònica</creatorcontrib><creatorcontrib>Melero, Roberto</creatorcontrib><creatorcontrib>Paradela, Alberto</creatorcontrib><creatorcontrib>Carrascosa, José L.</creatorcontrib><creatorcontrib>Carazo, José-Maria</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Busselez, Johan</au><au>Chichón, Francisco Javier</au><au>Rodríguez, Maria Josefa</au><au>Alpízar, Adan</au><au>Gharbi, Séverine Isabelle</au><au>Franch, Mònica</au><au>Melero, Roberto</au><au>Paradela, Alberto</au><au>Carrascosa, José L.</au><au>Carazo, José-Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-05-10</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>7187</spage><epage>7187</epage><pages>7187-7187</pages><artnum>7187</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>We have used cryo Electron Tomography, proteomics and immunolabeling to study centrosomes isolated from the young lamb thymus, an efficient source of quiescent differentiated cells. We compared the proteome of thymocyte centrosomes to data published for KE37 cells, focusing on proteins associated with centriole disengagement and centrosome separation. The data obtained enhances our understanding of the protein system joining the centrioles, a system comprised of a branched network of fibers linked to an apparently amorphous density that was partially characterized here. A number of proteins were localized to the amorphous density by immunolabeling (C-NAP1, cohesin SMC1, condensin SMC4 and NCAPD2), yet not DNA. In conjuction, these data not only extend our understanding of centrosomes but they will help refine the model that focus on the protein system associated with the centriolar junction.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31076588</pmid><doi>10.1038/s41598-019-43338-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0788-8447</orcidid><orcidid>https://orcid.org/0000-0002-4078-1265</orcidid><orcidid>https://orcid.org/0000-0003-2766-0327</orcidid><orcidid>https://orcid.org/0000-0001-6837-7056</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2019-05, Vol.9 (1), p.7187-7187, Article 7187 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6510768 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 101/28 14/1 14/19 38/79 631/114/2784 631/535/1258/1260 631/80/641 82/58 82/83 Animals Cell Line Centrioles Centrosome - metabolism Centrosomes Cohesin Condensin Cryoelectron Microscopy Electron Microscope Tomography Fibers Gene Regulatory Networks Humanities and Social Sciences multidisciplinary Proteomics Proteomics - methods Science Science (multidisciplinary) Sheep Thymocytes Thymocytes - cytology Thymocytes - metabolism Thymus Tomography |
| title | Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T20%3A21%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cryo-Electron%20Tomography%20and%20Proteomics%20studies%20of%20centrosomes%20from%20differentiated%20quiescent%20thymocytes&rft.jtitle=Scientific%20reports&rft.au=Busselez,%20Johan&rft.date=2019-05-10&rft.volume=9&rft.issue=1&rft.spage=7187&rft.epage=7187&rft.pages=7187-7187&rft.artnum=7187&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-43338-9&rft_dat=%3Cproquest_pubme%3E2231910751%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2222924986&rft_id=info:pmid/31076588&rfr_iscdi=true |