Anti-diarrheal effect of Scutellaria baicalensis is associated with suppression of smooth muscle in the rat colon
( ) has been used to manage diarrhea, and its anti-inflammatory effects are responsible for anti-diarrheal effects. However, there are no data concerning its direct effect on colonic motility. Therefore, the effects of the major components of (baicalin, baicalein and wogonin) on colonic motility wer...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2019-06, Vol.17 (6), p.4748-4756 |
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| creator | Kim, Hyun Ju La, Jun-Ho Kim, Hee Man Yang, Il-Suk Sung, Tae Sik |
| description | (
) has been used to manage diarrhea, and its anti-inflammatory effects are responsible for anti-diarrheal effects. However, there are no data concerning its direct effect on colonic motility. Therefore, the effects of the major components of
(baicalin, baicalein and wogonin) on colonic motility were investigated. A segment of the distal colon of rats was placed in Krebs solution to monitor spontaneous giant contractions (GCs). Changes in GCs were recorded after applying baicalin, baicalein or wogonin. After pretreatment with N
-nitro-L-arginine methyl ester hydrochloride (L-NAME), 1H-(1,2,4)-oxadiazolo (4,2-a) quinoxalin-1-one (ODQ), tetradotoxin, w-conotoxin, apamin, and iberiotoxin, changes in GCs by wogonin were recorded and analyzed. The segment of the distal colon showed spontaneous GCs at a mean amplitude of 3.7±0.3 g with a frequency of 0.8±0.1/min. Baicalin, baicalein, and wogonin reduced both the amplitude and the frequency of GCs in a dose-dependent manner. Wogonin had the most potent inhibitory effect on GCs (IC
was 14.6 µM in amplitude and 14.2 µM in frequency). Wogonin-induced GC reduction was not significantly affected by the inhibition of nitric oxide/cGMP pathways with L-NAME and ODQ. Blocking the enteric neurotransmission with tetradotoxin and ω-conotoxin was ineffective on the wogonin-induced reduction of GCs. Ca
-activated K
(K
) channel blockers (apamin and iberiotoxin) significantly attenuated the inhibitory effects of wogonin on GCs (P |
| doi_str_mv | 10.3892/etm.2019.7469 |
| format | Article |
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) has been used to manage diarrhea, and its anti-inflammatory effects are responsible for anti-diarrheal effects. However, there are no data concerning its direct effect on colonic motility. Therefore, the effects of the major components of
(baicalin, baicalein and wogonin) on colonic motility were investigated. A segment of the distal colon of rats was placed in Krebs solution to monitor spontaneous giant contractions (GCs). Changes in GCs were recorded after applying baicalin, baicalein or wogonin. After pretreatment with N
-nitro-L-arginine methyl ester hydrochloride (L-NAME), 1H-(1,2,4)-oxadiazolo (4,2-a) quinoxalin-1-one (ODQ), tetradotoxin, w-conotoxin, apamin, and iberiotoxin, changes in GCs by wogonin were recorded and analyzed. The segment of the distal colon showed spontaneous GCs at a mean amplitude of 3.7±0.3 g with a frequency of 0.8±0.1/min. Baicalin, baicalein, and wogonin reduced both the amplitude and the frequency of GCs in a dose-dependent manner. Wogonin had the most potent inhibitory effect on GCs (IC
was 14.6 µM in amplitude and 14.2 µM in frequency). Wogonin-induced GC reduction was not significantly affected by the inhibition of nitric oxide/cGMP pathways with L-NAME and ODQ. Blocking the enteric neurotransmission with tetradotoxin and ω-conotoxin was ineffective on the wogonin-induced reduction of GCs. Ca
-activated K
(K
) channel blockers (apamin and iberiotoxin) significantly attenuated the inhibitory effects of wogonin on GCs (P<0.01). Wogonin was effective in inhibiting colonic motility, probably through the opening of K
channels located in the smooth muscle apparatus. These findings suggest that wogonin may be a candidate drug for the management of dysmotility-related diarrhea.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2019.7469</identifier><identifier>PMID: 31105793</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Anti-inflammatory agents ; Arginine ; Colon ; Diarrhea ; Dosage and administration ; Drug dosages ; Drug therapy ; Health aspects ; Irritable bowel syndrome ; Laboratory animals ; Marine toxins ; Motility ; Oxidation-reduction reactions ; Rodents ; Skullcap ; Smooth muscle</subject><ispartof>Experimental and therapeutic medicine, 2019-06, Vol.17 (6), p.4748-4756</ispartof><rights>COPYRIGHT 2019 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2019</rights><rights>Copyright © 2019, Spandidos Publications 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-6c47cb7c32f68fda08ada70257612bf49e8a62f8dca8b2b23b8fff22d545d3123</citedby><cites>FETCH-LOGICAL-c412t-6c47cb7c32f68fda08ada70257612bf49e8a62f8dca8b2b23b8fff22d545d3123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507508/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507508/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31105793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyun Ju</creatorcontrib><creatorcontrib>La, Jun-Ho</creatorcontrib><creatorcontrib>Kim, Hee Man</creatorcontrib><creatorcontrib>Yang, Il-Suk</creatorcontrib><creatorcontrib>Sung, Tae Sik</creatorcontrib><title>Anti-diarrheal effect of Scutellaria baicalensis is associated with suppression of smooth muscle in the rat colon</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>(
) has been used to manage diarrhea, and its anti-inflammatory effects are responsible for anti-diarrheal effects. However, there are no data concerning its direct effect on colonic motility. Therefore, the effects of the major components of
(baicalin, baicalein and wogonin) on colonic motility were investigated. A segment of the distal colon of rats was placed in Krebs solution to monitor spontaneous giant contractions (GCs). Changes in GCs were recorded after applying baicalin, baicalein or wogonin. After pretreatment with N
-nitro-L-arginine methyl ester hydrochloride (L-NAME), 1H-(1,2,4)-oxadiazolo (4,2-a) quinoxalin-1-one (ODQ), tetradotoxin, w-conotoxin, apamin, and iberiotoxin, changes in GCs by wogonin were recorded and analyzed. The segment of the distal colon showed spontaneous GCs at a mean amplitude of 3.7±0.3 g with a frequency of 0.8±0.1/min. Baicalin, baicalein, and wogonin reduced both the amplitude and the frequency of GCs in a dose-dependent manner. Wogonin had the most potent inhibitory effect on GCs (IC
was 14.6 µM in amplitude and 14.2 µM in frequency). Wogonin-induced GC reduction was not significantly affected by the inhibition of nitric oxide/cGMP pathways with L-NAME and ODQ. Blocking the enteric neurotransmission with tetradotoxin and ω-conotoxin was ineffective on the wogonin-induced reduction of GCs. Ca
-activated K
(K
) channel blockers (apamin and iberiotoxin) significantly attenuated the inhibitory effects of wogonin on GCs (P<0.01). Wogonin was effective in inhibiting colonic motility, probably through the opening of K
channels located in the smooth muscle apparatus. These findings suggest that wogonin may be a candidate drug for the management of dysmotility-related diarrhea.</description><subject>Anti-inflammatory agents</subject><subject>Arginine</subject><subject>Colon</subject><subject>Diarrhea</subject><subject>Dosage and administration</subject><subject>Drug dosages</subject><subject>Drug therapy</subject><subject>Health aspects</subject><subject>Irritable bowel syndrome</subject><subject>Laboratory animals</subject><subject>Marine toxins</subject><subject>Motility</subject><subject>Oxidation-reduction reactions</subject><subject>Rodents</subject><subject>Skullcap</subject><subject>Smooth muscle</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptUk1rHSEUldLShDTLbovQTTfz6sc4OpvCI7RpIZBFkrU4zjXPMKMv6jT039chL2lTqoJyPedc7uEg9J6SDVc9-wxl3jBC-41su_4VOqayZw0lVLw-vEmv6BE6zfmO1CU6qpR4i444pUTInh-j-20ovhm9SWkHZsLgHNiCo8NXdikwTSZ5gwfjrZkgZJ9xPSbnaL0pMOIHX3Y4L_t9gpx9DCszzzHW6rxkOwH2AZcd4GQKtnGK4R1648yU4fRwn6Cbb1-vz743F5fnP862F41tKStNZ1tpB2k5c51yoyHKjEYSJmRH2eDaHpTpmFOjNWpgA-ODcs4xNopWjJwyfoK-POrul2GG0UIoyUx6n_xs0i8djdcvf4Lf6dv4U3eCSEFUFfh0EEjxfoFc9OyzXS0JEJesGeOsukgprdCP_0Dv4pJCHa-iGG87yaT8g7qtXmofXKx97Sqqt0IpxXvVr203_0HVPcLsbQzgfK2_IDSPBJtizgnc84yU6DUmusZErzHRa0wq_sPfxjyjn0LBfwNwg7mP</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Kim, Hyun Ju</creator><creator>La, Jun-Ho</creator><creator>Kim, Hee Man</creator><creator>Yang, Il-Suk</creator><creator>Sung, Tae Sik</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190601</creationdate><title>Anti-diarrheal effect of Scutellaria baicalensis is associated with suppression of smooth muscle in the rat colon</title><author>Kim, Hyun Ju ; La, Jun-Ho ; Kim, Hee Man ; Yang, Il-Suk ; Sung, Tae Sik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-6c47cb7c32f68fda08ada70257612bf49e8a62f8dca8b2b23b8fff22d545d3123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anti-inflammatory agents</topic><topic>Arginine</topic><topic>Colon</topic><topic>Diarrhea</topic><topic>Dosage and administration</topic><topic>Drug dosages</topic><topic>Drug therapy</topic><topic>Health aspects</topic><topic>Irritable bowel syndrome</topic><topic>Laboratory animals</topic><topic>Marine toxins</topic><topic>Motility</topic><topic>Oxidation-reduction reactions</topic><topic>Rodents</topic><topic>Skullcap</topic><topic>Smooth muscle</topic><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyun Ju</creatorcontrib><creatorcontrib>La, Jun-Ho</creatorcontrib><creatorcontrib>Kim, Hee Man</creatorcontrib><creatorcontrib>Yang, Il-Suk</creatorcontrib><creatorcontrib>Sung, Tae Sik</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyun Ju</au><au>La, Jun-Ho</au><au>Kim, Hee Man</au><au>Yang, Il-Suk</au><au>Sung, Tae Sik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-diarrheal effect of Scutellaria baicalensis is associated with suppression of smooth muscle in the rat colon</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>17</volume><issue>6</issue><spage>4748</spage><epage>4756</epage><pages>4748-4756</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>(
) has been used to manage diarrhea, and its anti-inflammatory effects are responsible for anti-diarrheal effects. However, there are no data concerning its direct effect on colonic motility. Therefore, the effects of the major components of
(baicalin, baicalein and wogonin) on colonic motility were investigated. A segment of the distal colon of rats was placed in Krebs solution to monitor spontaneous giant contractions (GCs). Changes in GCs were recorded after applying baicalin, baicalein or wogonin. After pretreatment with N
-nitro-L-arginine methyl ester hydrochloride (L-NAME), 1H-(1,2,4)-oxadiazolo (4,2-a) quinoxalin-1-one (ODQ), tetradotoxin, w-conotoxin, apamin, and iberiotoxin, changes in GCs by wogonin were recorded and analyzed. The segment of the distal colon showed spontaneous GCs at a mean amplitude of 3.7±0.3 g with a frequency of 0.8±0.1/min. Baicalin, baicalein, and wogonin reduced both the amplitude and the frequency of GCs in a dose-dependent manner. Wogonin had the most potent inhibitory effect on GCs (IC
was 14.6 µM in amplitude and 14.2 µM in frequency). Wogonin-induced GC reduction was not significantly affected by the inhibition of nitric oxide/cGMP pathways with L-NAME and ODQ. Blocking the enteric neurotransmission with tetradotoxin and ω-conotoxin was ineffective on the wogonin-induced reduction of GCs. Ca
-activated K
(K
) channel blockers (apamin and iberiotoxin) significantly attenuated the inhibitory effects of wogonin on GCs (P<0.01). Wogonin was effective in inhibiting colonic motility, probably through the opening of K
channels located in the smooth muscle apparatus. These findings suggest that wogonin may be a candidate drug for the management of dysmotility-related diarrhea.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>31105793</pmid><doi>10.3892/etm.2019.7469</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central |
| subjects | Anti-inflammatory agents Arginine Colon Diarrhea Dosage and administration Drug dosages Drug therapy Health aspects Irritable bowel syndrome Laboratory animals Marine toxins Motility Oxidation-reduction reactions Rodents Skullcap Smooth muscle |
| title | Anti-diarrheal effect of Scutellaria baicalensis is associated with suppression of smooth muscle in the rat colon |
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