Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

Leaf samples of Cucumis Sativus L. ( C. sativus ) (Family; Cucurbitaceae ) showing vein thickening, mild leaf curling and leaf enations were collected from the farmer’s field. Amplification of the full-length viral molecules was performed through rolling circle amplification (RCA). Cloning of the fu...

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