Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens
The Aries assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of and nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray...
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| Veröffentlicht in: | Journal of clinical microbiology 2019-05, Vol.57 (5) |
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| container_title | Journal of clinical microbiology |
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| creator | McMillen, T Chow, H Y Das, Shubhagata Dunbar, Sherry A Babady, N E |
| description | The Aries
assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of
and
nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray respiratory panel (RP). The Aries BA was evaluated using retrospective, remnant nasopharyngeal swabs (NPS), previously tested by FilmArray RP. Performance characteristics evaluated included positive percent agreement (PPA) and negative percent agreement (NPA) with the FilmArray RP. Discordant analysis was performed using bidirectional sequencing. A time and motion study was performed to compare the laboratory workflow of the two tests. Three hundred samples were included in the study. There were no samples positive for
The PPA and NPA of the Aries BA were 61.1% (95% confidence interval [CI], 35.8 to 82.7%) and 100% (95% CI, 98.7 to 100%). Discordant results included five
results that were incorrectly identified as
by the FilmArray RP and one false-negative result for both the Aries BA and the FilmArray RP. The overall agreement between the Aries BA and FilmArray RP for the detection of
was considered good at 97.7% with a kappa value of 0.71 (95% CI, 0.51 to 0.9). The Aries BA offers a new diagnostic option for the rapid and targeted approach to the diagnosis of pertussis. Unlike the FilmArray RP, the Aries BA did not cross-react with
in our study, although a larger sample set should be tested to confirm this finding. |
| doi_str_mv | 10.1128/JCM.01966-18 |
| format | Article |
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assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of
and
nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray respiratory panel (RP). The Aries BA was evaluated using retrospective, remnant nasopharyngeal swabs (NPS), previously tested by FilmArray RP. Performance characteristics evaluated included positive percent agreement (PPA) and negative percent agreement (NPA) with the FilmArray RP. Discordant analysis was performed using bidirectional sequencing. A time and motion study was performed to compare the laboratory workflow of the two tests. Three hundred samples were included in the study. There were no samples positive for
The PPA and NPA of the Aries BA were 61.1% (95% confidence interval [CI], 35.8 to 82.7%) and 100% (95% CI, 98.7 to 100%). Discordant results included five
results that were incorrectly identified as
by the FilmArray RP and one false-negative result for both the Aries BA and the FilmArray RP. The overall agreement between the Aries BA and FilmArray RP for the detection of
was considered good at 97.7% with a kappa value of 0.71 (95% CI, 0.51 to 0.9). The Aries BA offers a new diagnostic option for the rapid and targeted approach to the diagnosis of pertussis. Unlike the FilmArray RP, the Aries BA did not cross-react with
in our study, although a larger sample set should be tested to confirm this finding.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01966-18</identifier><identifier>PMID: 30787143</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Bacteriology</subject><ispartof>Journal of clinical microbiology, 2019-05, Vol.57 (5)</ispartof><rights>Copyright © 2019 American Society for Microbiology.</rights><rights>Copyright © 2019 American Society for Microbiology. 2019 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-55abd8f8a526df77cb6e5044346a1d61dd5fa59bcf0bb23d3d207d5f69cc1d283</citedby><cites>FETCH-LOGICAL-c384t-55abd8f8a526df77cb6e5044346a1d61dd5fa59bcf0bb23d3d207d5f69cc1d283</cites><orcidid>0000-0003-4399-1249</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498007/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498007/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30787143$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McMillen, T</creatorcontrib><creatorcontrib>Chow, H Y</creatorcontrib><creatorcontrib>Das, Shubhagata</creatorcontrib><creatorcontrib>Dunbar, Sherry A</creatorcontrib><creatorcontrib>Babady, N E</creatorcontrib><title>Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The Aries
assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of
and
nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray respiratory panel (RP). The Aries BA was evaluated using retrospective, remnant nasopharyngeal swabs (NPS), previously tested by FilmArray RP. Performance characteristics evaluated included positive percent agreement (PPA) and negative percent agreement (NPA) with the FilmArray RP. Discordant analysis was performed using bidirectional sequencing. A time and motion study was performed to compare the laboratory workflow of the two tests. Three hundred samples were included in the study. There were no samples positive for
The PPA and NPA of the Aries BA were 61.1% (95% confidence interval [CI], 35.8 to 82.7%) and 100% (95% CI, 98.7 to 100%). Discordant results included five
results that were incorrectly identified as
by the FilmArray RP and one false-negative result for both the Aries BA and the FilmArray RP. The overall agreement between the Aries BA and FilmArray RP for the detection of
was considered good at 97.7% with a kappa value of 0.71 (95% CI, 0.51 to 0.9). The Aries BA offers a new diagnostic option for the rapid and targeted approach to the diagnosis of pertussis. Unlike the FilmArray RP, the Aries BA did not cross-react with
in our study, although a larger sample set should be tested to confirm this finding.</description><subject>Bacteriology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVUblOxDAUtBAIlqOjRi4pCNjxEadBWpZbHAUg0VmOD9YoGwc7AdHy5WQ5VlA9ad7MvHkaALYx2sc4FweXk-t9hEvOMyyWwAijUmSco8dlMEKoZBnGpFgD6yk9I4QpZWwVrBFUiAJTMgIfJ6-q7lXnQwODg93UwnH0NsGjEI3tbF0rOE5JvUMXIjweEP3FVY2BF8Y2nXdeL-R_RK2NXZ-ST9A38Eal0E5VfG-erKrh3Zuq4F1rtZ_ZJm2CFafqZLd-5gZ4OD25n5xnV7dnF5PxVaaJoF3GmKqMcEKxnBtXFLriliFKCeUKG46NYU6xstIOVVVODDE5KgaMl1pjkwuyAQ6_fdu-mlmjh_BR1bKNfjYkk0F5-X_T-Kl8Cq-S01IgVAwGuz8GMbz0NnVy5pOef9vY0CeZY0FZXua0HKh731QdQ0rRusUZjOS8NjnUJr9qk3gebedvtAX5tyfyCQ2KlvY</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>McMillen, T</creator><creator>Chow, H Y</creator><creator>Das, Shubhagata</creator><creator>Dunbar, Sherry A</creator><creator>Babady, N E</creator><general>American Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4399-1249</orcidid></search><sort><creationdate>20190501</creationdate><title>Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens</title><author>McMillen, T ; Chow, H Y ; Das, Shubhagata ; Dunbar, Sherry A ; Babady, N E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-55abd8f8a526df77cb6e5044346a1d61dd5fa59bcf0bb23d3d207d5f69cc1d283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacteriology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McMillen, T</creatorcontrib><creatorcontrib>Chow, H Y</creatorcontrib><creatorcontrib>Das, Shubhagata</creatorcontrib><creatorcontrib>Dunbar, Sherry A</creatorcontrib><creatorcontrib>Babady, N E</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McMillen, T</au><au>Chow, H Y</au><au>Das, Shubhagata</au><au>Dunbar, Sherry A</au><au>Babady, N E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2019-05-01</date><risdate>2019</risdate><volume>57</volume><issue>5</issue><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>The Aries
assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of
and
nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray respiratory panel (RP). The Aries BA was evaluated using retrospective, remnant nasopharyngeal swabs (NPS), previously tested by FilmArray RP. Performance characteristics evaluated included positive percent agreement (PPA) and negative percent agreement (NPA) with the FilmArray RP. Discordant analysis was performed using bidirectional sequencing. A time and motion study was performed to compare the laboratory workflow of the two tests. Three hundred samples were included in the study. There were no samples positive for
The PPA and NPA of the Aries BA were 61.1% (95% confidence interval [CI], 35.8 to 82.7%) and 100% (95% CI, 98.7 to 100%). Discordant results included five
results that were incorrectly identified as
by the FilmArray RP and one false-negative result for both the Aries BA and the FilmArray RP. The overall agreement between the Aries BA and FilmArray RP for the detection of
was considered good at 97.7% with a kappa value of 0.71 (95% CI, 0.51 to 0.9). The Aries BA offers a new diagnostic option for the rapid and targeted approach to the diagnosis of pertussis. Unlike the FilmArray RP, the Aries BA did not cross-react with
in our study, although a larger sample set should be tested to confirm this finding.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>30787143</pmid><doi>10.1128/JCM.01966-18</doi><orcidid>https://orcid.org/0000-0003-4399-1249</orcidid><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Bacteriology |
| title | Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens |
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