Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation

The phenomenon of protein aggregation capture (PAC) on a wide range of different microparticles is described. Exploiting this mechanism enables generation of clean peptide mixtures from cell lines, tissues, and protein pulldowns for proteomics, phosphoproteomics, and secretomics analysis. The findin...

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Veröffentlicht in:	Molecular & cellular proteomics 2019-05, Vol.18 (5), p.1027-1035
Hauptverfasser:	Batth, Tanveer S., Tollenaere, MaximA.X., Rüther, Patrick, Gonzalez-Franquesa, Alba, Prabhakar, Bhargav S., Bekker-Jensen, Simon, Deshmukh, Atul S., Olsen, Jesper V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity proteomics Animals Automation Cell Line, Tumor Cell-Derived Microparticles - metabolism Humans magnetic beads Mass Spectrometry Mice microparticles Phosphoproteome Protein Aggregates protein aggregation Protein Denaturation Protein Processing, Post-Translational Proteomics - methods RAW 264.7 Cells sample preparation Secretome Technological Innovation and Resources
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