Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project
Background This study measured longitudinal DNA methylation dynamics at growth-related genes during childhood, and then tested whether DNA methylation at various stages of childhood was associated with obesity status. Methods Using neonatal bloodspot ( n = 132) and matched childhood blood samples (...
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| Veröffentlicht in: | Pediatric research 2019-05, Vol.85 (6), p.848-855 |
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| creator | Kochmanski, Joseph Goodrich, Jaclyn M. Peterson, Karen E. Lumeng, Julie C. Dolinoy, Dana C. |
| description | Background
This study measured longitudinal DNA methylation dynamics at growth-related genes during childhood, and then tested whether DNA methylation at various stages of childhood was associated with obesity status.
Methods
Using neonatal bloodspot (
n
= 132) and matched childhood blood samples (
n
= 65), DNA methylation was quantified at a repetitive element (long interspersed nuclear element-1 (LINE-1)), two imprinted genes (
IGF2
,
H19
), and four non-imprinted genes (
LEP
,
PPARA
,
ESR1
,
SREBF1
) related to growth and adiposity. Logistic regression was used to test whether neonatal bloodspot DNA methylation at target genes was associated with log odds of obesity (Y/N) in children recruited from three age groups—12–24 months old (
n
= 40), 3–5 years of age (
n
= 40), and 10–12 years of age (
n
= 52).
Results
In 3–5 year olds, neonatal bloodspot LINE-1 methylation was negatively associated with obesity (log odds = −0.40,
p
= 0.04). Across childhood age group in matched blood samples, DNA methylation levels in blood decreased (
p
|
| doi_str_mv | 10.1038/s41390-018-0227-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6494701</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2217460125</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-e9dada29bafdb4659284772e893a516f3095eb27b2f2e339b3963854c470faab3</originalsourceid><addsrcrecordid>eNp1kUtv1DAUhS1ERYeBH8AGWWLDJuBXHt4gVYVSpKrtAtbWTXIz8ciJB9vpqP8ej6aUh9SVJd9zPt_jQ8gbzj5wJpuPUXGpWcF4UzAh6oI_IyteynyjVP2crBiTvJBaN6fkZYxbxrgqG_WCnEqmRCmlXpH9NfoZEjjaOu_7uPOJfr4-oxOm8d5Bsn6mO0gJwxwpBKQQo-8sJOzp3qaRdqN1_ZitdI92MyYaE6QlUjvTNCK9RHCZRC9gss5ipLfBb7FLr8jJAC7i64dzTX5cfPl-fllc3Xz9dn52VXSqZqlA3UMPQrcw9K2qSi0aVdcCGy2h5NUgmS6xFXUrBoE5UCt1JZtSHdwDQCvX5NORu1vaCfsO5xTAmV2wE4R748GafyezHc3G35lK6czgGfD-ARD8zwVjMpONHToHM_olGsGlVJJVlc7Sd_9Jt34Jc45nhOC1qhjPv74m_Kjqgo8x4PC4DGfmUKs51mpyreZQqzks8fbvFI-O3z1mgTgKYh7NGwx_nn6a-gsbPa-r</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2217460125</pqid></control><display><type>article</type><title>Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Kochmanski, Joseph ; Goodrich, Jaclyn M. ; Peterson, Karen E. ; Lumeng, Julie C. ; Dolinoy, Dana C.</creator><creatorcontrib>Kochmanski, Joseph ; Goodrich, Jaclyn M. ; Peterson, Karen E. ; Lumeng, Julie C. ; Dolinoy, Dana C.</creatorcontrib><description>Background
This study measured longitudinal DNA methylation dynamics at growth-related genes during childhood, and then tested whether DNA methylation at various stages of childhood was associated with obesity status.
Methods
Using neonatal bloodspot (
n
= 132) and matched childhood blood samples (
n
= 65), DNA methylation was quantified at a repetitive element (long interspersed nuclear element-1 (LINE-1)), two imprinted genes (
IGF2
,
H19
), and four non-imprinted genes (
LEP
,
PPARA
,
ESR1
,
SREBF1
) related to growth and adiposity. Logistic regression was used to test whether neonatal bloodspot DNA methylation at target genes was associated with log odds of obesity (Y/N) in children recruited from three age groups—12–24 months old (
n
= 40), 3–5 years of age (
n
= 40), and 10–12 years of age (
n
= 52).
Results
In 3–5 year olds, neonatal bloodspot LINE-1 methylation was negatively associated with obesity (log odds = −0.40,
p
= 0.04). Across childhood age group in matched blood samples, DNA methylation levels in blood decreased (
p
< 0.05) at LINE-1,
PPARA
,
ESR1
,
SREBF1
,
IGF2
, and
H19
, and increased (
p
< 0.05) at
LEP
.
Conclusions
Our results suggest that age-related epigenetic changes occur at growth-related genes in the first decade of life, and that gene-specific neonatal bloodspot DNA methylation may be a useful biomarker of obesity likelihood during childhood.</description><identifier>ISSN: 0031-3998</identifier><identifier>ISSN: 1530-0447</identifier><identifier>EISSN: 1530-0447</identifier><identifier>DOI: 10.1038/s41390-018-0227-1</identifier><identifier>PMID: 30425339</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Age ; Age Factors ; Basic Science Article ; Body Weight - genetics ; Child ; Child, Preschool ; Childhood ; Deoxyribonucleic acid ; DNA ; DNA Methylation ; Epigenesis, Genetic ; Female ; Fetal Blood - metabolism ; Genetic Markers ; Genomic Imprinting ; Humans ; Infant ; Infant, Newborn ; Insulin-Like Growth Factor II - genetics ; Logistic Models ; Long Interspersed Nucleotide Elements ; Male ; Medicine ; Medicine & Public Health ; Obesity ; Pediatric Obesity - blood ; Pediatric Obesity - etiology ; Pediatric Obesity - genetics ; Pediatric Surgery ; Pediatrics ; Risk Factors</subject><ispartof>Pediatric research, 2019-05, Vol.85 (6), p.848-855</ispartof><rights>International Pediatric Research Foundation, Inc. 2018</rights><rights>Copyright Nature Publishing Group May 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-e9dada29bafdb4659284772e893a516f3095eb27b2f2e339b3963854c470faab3</citedby><cites>FETCH-LOGICAL-c470t-e9dada29bafdb4659284772e893a516f3095eb27b2f2e339b3963854c470faab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41390-018-0227-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41390-018-0227-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30425339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kochmanski, Joseph</creatorcontrib><creatorcontrib>Goodrich, Jaclyn M.</creatorcontrib><creatorcontrib>Peterson, Karen E.</creatorcontrib><creatorcontrib>Lumeng, Julie C.</creatorcontrib><creatorcontrib>Dolinoy, Dana C.</creatorcontrib><title>Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project</title><title>Pediatric research</title><addtitle>Pediatr Res</addtitle><addtitle>Pediatr Res</addtitle><description>Background
This study measured longitudinal DNA methylation dynamics at growth-related genes during childhood, and then tested whether DNA methylation at various stages of childhood was associated with obesity status.
Methods
Using neonatal bloodspot (
n
= 132) and matched childhood blood samples (
n
= 65), DNA methylation was quantified at a repetitive element (long interspersed nuclear element-1 (LINE-1)), two imprinted genes (
IGF2
,
H19
), and four non-imprinted genes (
LEP
,
PPARA
,
ESR1
,
SREBF1
) related to growth and adiposity. Logistic regression was used to test whether neonatal bloodspot DNA methylation at target genes was associated with log odds of obesity (Y/N) in children recruited from three age groups—12–24 months old (
n
= 40), 3–5 years of age (
n
= 40), and 10–12 years of age (
n
= 52).
Results
In 3–5 year olds, neonatal bloodspot LINE-1 methylation was negatively associated with obesity (log odds = −0.40,
p
= 0.04). Across childhood age group in matched blood samples, DNA methylation levels in blood decreased (
p
< 0.05) at LINE-1,
PPARA
,
ESR1
,
SREBF1
,
IGF2
, and
H19
, and increased (
p
< 0.05) at
LEP
.
Conclusions
Our results suggest that age-related epigenetic changes occur at growth-related genes in the first decade of life, and that gene-specific neonatal bloodspot DNA methylation may be a useful biomarker of obesity likelihood during childhood.</description><subject>Age</subject><subject>Age Factors</subject><subject>Basic Science Article</subject><subject>Body Weight - genetics</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Childhood</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>Female</subject><subject>Fetal Blood - metabolism</subject><subject>Genetic Markers</subject><subject>Genomic Imprinting</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Logistic Models</subject><subject>Long Interspersed Nucleotide Elements</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Obesity</subject><subject>Pediatric Obesity - blood</subject><subject>Pediatric Obesity - etiology</subject><subject>Pediatric Obesity - genetics</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Risk Factors</subject><issn>0031-3998</issn><issn>1530-0447</issn><issn>1530-0447</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kUtv1DAUhS1ERYeBH8AGWWLDJuBXHt4gVYVSpKrtAtbWTXIz8ciJB9vpqP8ej6aUh9SVJd9zPt_jQ8gbzj5wJpuPUXGpWcF4UzAh6oI_IyteynyjVP2crBiTvJBaN6fkZYxbxrgqG_WCnEqmRCmlXpH9NfoZEjjaOu_7uPOJfr4-oxOm8d5Bsn6mO0gJwxwpBKQQo-8sJOzp3qaRdqN1_ZitdI92MyYaE6QlUjvTNCK9RHCZRC9gss5ipLfBb7FLr8jJAC7i64dzTX5cfPl-fllc3Xz9dn52VXSqZqlA3UMPQrcw9K2qSi0aVdcCGy2h5NUgmS6xFXUrBoE5UCt1JZtSHdwDQCvX5NORu1vaCfsO5xTAmV2wE4R748GafyezHc3G35lK6czgGfD-ARD8zwVjMpONHToHM_olGsGlVJJVlc7Sd_9Jt34Jc45nhOC1qhjPv74m_Kjqgo8x4PC4DGfmUKs51mpyreZQqzks8fbvFI-O3z1mgTgKYh7NGwx_nn6a-gsbPa-r</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Kochmanski, Joseph</creator><creator>Goodrich, Jaclyn M.</creator><creator>Peterson, Karen E.</creator><creator>Lumeng, Julie C.</creator><creator>Dolinoy, Dana C.</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190501</creationdate><title>Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project</title><author>Kochmanski, Joseph ; Goodrich, Jaclyn M. ; Peterson, Karen E. ; Lumeng, Julie C. ; Dolinoy, Dana C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-e9dada29bafdb4659284772e893a516f3095eb27b2f2e339b3963854c470faab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Age</topic><topic>Age Factors</topic><topic>Basic Science Article</topic><topic>Body Weight - genetics</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Childhood</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Methylation</topic><topic>Epigenesis, Genetic</topic><topic>Female</topic><topic>Fetal Blood - metabolism</topic><topic>Genetic Markers</topic><topic>Genomic Imprinting</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Logistic Models</topic><topic>Long Interspersed Nucleotide Elements</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Obesity</topic><topic>Pediatric Obesity - blood</topic><topic>Pediatric Obesity - etiology</topic><topic>Pediatric Obesity - genetics</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Risk Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kochmanski, Joseph</creatorcontrib><creatorcontrib>Goodrich, Jaclyn M.</creatorcontrib><creatorcontrib>Peterson, Karen E.</creatorcontrib><creatorcontrib>Lumeng, Julie C.</creatorcontrib><creatorcontrib>Dolinoy, Dana C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pediatric research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kochmanski, Joseph</au><au>Goodrich, Jaclyn M.</au><au>Peterson, Karen E.</au><au>Lumeng, Julie C.</au><au>Dolinoy, Dana C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project</atitle><jtitle>Pediatric research</jtitle><stitle>Pediatr Res</stitle><addtitle>Pediatr Res</addtitle><date>2019-05-01</date><risdate>2019</risdate><volume>85</volume><issue>6</issue><spage>848</spage><epage>855</epage><pages>848-855</pages><issn>0031-3998</issn><issn>1530-0447</issn><eissn>1530-0447</eissn><abstract>Background
This study measured longitudinal DNA methylation dynamics at growth-related genes during childhood, and then tested whether DNA methylation at various stages of childhood was associated with obesity status.
Methods
Using neonatal bloodspot (
n
= 132) and matched childhood blood samples (
n
= 65), DNA methylation was quantified at a repetitive element (long interspersed nuclear element-1 (LINE-1)), two imprinted genes (
IGF2
,
H19
), and four non-imprinted genes (
LEP
,
PPARA
,
ESR1
,
SREBF1
) related to growth and adiposity. Logistic regression was used to test whether neonatal bloodspot DNA methylation at target genes was associated with log odds of obesity (Y/N) in children recruited from three age groups—12–24 months old (
n
= 40), 3–5 years of age (
n
= 40), and 10–12 years of age (
n
= 52).
Results
In 3–5 year olds, neonatal bloodspot LINE-1 methylation was negatively associated with obesity (log odds = −0.40,
p
= 0.04). Across childhood age group in matched blood samples, DNA methylation levels in blood decreased (
p
< 0.05) at LINE-1,
PPARA
,
ESR1
,
SREBF1
,
IGF2
, and
H19
, and increased (
p
< 0.05) at
LEP
.
Conclusions
Our results suggest that age-related epigenetic changes occur at growth-related genes in the first decade of life, and that gene-specific neonatal bloodspot DNA methylation may be a useful biomarker of obesity likelihood during childhood.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>30425339</pmid><doi>10.1038/s41390-018-0227-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Pediatric research, 2019-05, Vol.85 (6), p.848-855 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Age Age Factors Basic Science Article Body Weight - genetics Child Child, Preschool Childhood Deoxyribonucleic acid DNA DNA Methylation Epigenesis, Genetic Female Fetal Blood - metabolism Genetic Markers Genomic Imprinting Humans Infant Infant, Newborn Insulin-Like Growth Factor II - genetics Logistic Models Long Interspersed Nucleotide Elements Male Medicine Medicine & Public Health Obesity Pediatric Obesity - blood Pediatric Obesity - etiology Pediatric Obesity - genetics Pediatric Surgery Pediatrics Risk Factors |
| title | Neonatal bloodspot DNA methylation patterns are associated with childhood weight status in the Healthy Families Project |
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