Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated. This ret...
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| creator | Lu, Xin Zeng, Mei Xu, Jialiang Zhou, Haijian Gu, Baoke Li, Zhenpeng Jin, Huiming Wang, Xiaoxun Zhang, Wen Hu, Yongfei Xiao, Wenjia Zhu, Baoli Xu, Xuebin Kan, Biao |
| description | Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated.
This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes.
Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged |
| doi_str_mv | 10.1016/j.ebiom.2019.03.006 |
| format | Article |
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This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes.
Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5 years. All strains, including fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing strains, were multi-drug resistant. S. Typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. Phylogeny based on the genomes combined with complete plasmid sequences revealed some clusters, suggesting the presence of mcr-1-harbouring Salmonella outbreaks in the community. Most mcr-1-positive strains were clustered together with the pork strains, strongly suggesting pork consumption as a main infection source.
The mcr-1-harbouring Salmonella prevalence in community-acquired diarrhoea displays a rapid increase trend, and the ESBL-mcr-1-harbouring Salmonella poses a threat for children. These findings highlight the necessary and significance of prohibiting colistin use in animals and continuous monitoring of mcr-1-harbouring Salmonella.</description><identifier>ISSN: 2352-3964</identifier><identifier>EISSN: 2352-3964</identifier><identifier>DOI: 10.1016/j.ebiom.2019.03.006</identifier><identifier>PMID: 30905850</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; Child ; Child, Preschool ; Children ; China - epidemiology ; Diarrhea - epidemiology ; Diarrhea - history ; Diarrhea - microbiology ; Female ; Genes, Bacterial ; Genome, Bacterial ; Genomics - methods ; History, 21st Century ; Humans ; Infant ; Male ; mcr-1 ; Microbial Sensitivity Tests ; Molecular Typing ; Multi-drug resistant ; Outbreak ; Outpatients ; Phylogeny ; Plasmids - genetics ; Public Health Surveillance ; Research paper ; Salmonella ; Salmonella - classification ; Salmonella - drug effects ; Salmonella - genetics ; Salmonella Infections - epidemiology ; Salmonella Infections - history ; Salmonella Infections - microbiology ; Serogroup ; Swine</subject><ispartof>EBioMedicine, 2019-04, Vol.42, p.133-144</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier B.V.</rights><rights>2019 The Authors. Published by Elsevier B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-9e9c2aa442cc44f211d26197693bf37ad2969978e6aff62d7b74fe14b3e28d593</citedby><cites>FETCH-LOGICAL-c459t-9e9c2aa442cc44f211d26197693bf37ad2969978e6aff62d7b74fe14b3e28d593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491383/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491383/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30905850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Xin</creatorcontrib><creatorcontrib>Zeng, Mei</creatorcontrib><creatorcontrib>Xu, Jialiang</creatorcontrib><creatorcontrib>Zhou, Haijian</creatorcontrib><creatorcontrib>Gu, Baoke</creatorcontrib><creatorcontrib>Li, Zhenpeng</creatorcontrib><creatorcontrib>Jin, Huiming</creatorcontrib><creatorcontrib>Wang, Xiaoxun</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Hu, Yongfei</creatorcontrib><creatorcontrib>Xiao, Wenjia</creatorcontrib><creatorcontrib>Zhu, Baoli</creatorcontrib><creatorcontrib>Xu, Xuebin</creatorcontrib><creatorcontrib>Kan, Biao</creatorcontrib><title>Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016</title><title>EBioMedicine</title><addtitle>EBioMedicine</addtitle><description>Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated.
This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes.
Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5 years. All strains, including fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing strains, were multi-drug resistant. S. Typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. Phylogeny based on the genomes combined with complete plasmid sequences revealed some clusters, suggesting the presence of mcr-1-harbouring Salmonella outbreaks in the community. Most mcr-1-positive strains were clustered together with the pork strains, strongly suggesting pork consumption as a main infection source.
The mcr-1-harbouring Salmonella prevalence in community-acquired diarrhoea displays a rapid increase trend, and the ESBL-mcr-1-harbouring Salmonella poses a threat for children. These findings highlight the necessary and significance of prohibiting colistin use in animals and continuous monitoring of mcr-1-harbouring Salmonella.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>China - epidemiology</subject><subject>Diarrhea - epidemiology</subject><subject>Diarrhea - history</subject><subject>Diarrhea - microbiology</subject><subject>Female</subject><subject>Genes, Bacterial</subject><subject>Genome, Bacterial</subject><subject>Genomics - methods</subject><subject>History, 21st Century</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>mcr-1</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Typing</subject><subject>Multi-drug resistant</subject><subject>Outbreak</subject><subject>Outpatients</subject><subject>Phylogeny</subject><subject>Plasmids - genetics</subject><subject>Public Health Surveillance</subject><subject>Research paper</subject><subject>Salmonella</subject><subject>Salmonella - classification</subject><subject>Salmonella - drug effects</subject><subject>Salmonella - genetics</subject><subject>Salmonella Infections - epidemiology</subject><subject>Salmonella Infections - history</subject><subject>Salmonella Infections - microbiology</subject><subject>Serogroup</subject><subject>Swine</subject><issn>2352-3964</issn><issn>2352-3964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctq3TAUNKWlCUm-oFC07CJ29bJsLVool_QBgSzSroUsHdu62NKtZAe6KOQf8of9kurmpiHZdKWDNDNnNFMUbwiuCCbi_baCzoW5opjICrMKY_GiOKaspiWTgr98Mh8VZyltMcak5vmyfV0cMSxx3db4uPh9sXMWZhemMDiDtLdoAB_mPDuf3DAuCQWPZhNLUo46dmGNzg_oWk9z8DBNGvUxzMg6HeMYQE8orMtOLw58pjqPrkfth1G7c7QZndfniGavf27vsnNxWrzq9ZTg7OE8KX58vvi--VpeXn35tvl0WRpey6WUIA3VmnNqDOc9JcRSQWQjJOt61mhLpZCyaUHovhfUNl3DeyC8Y0BbW0t2Unw86O7WbgZrsreoJ7WLbtbxlwraqecv3o1qCDdKcElYy7LAuweBGH6ukBY1u2T23_cQ1qRodsMoY02boewANTGkFKF_XEOw2nentuq-O7XvTmGmch6Z9fapw0fOv6Yy4MMBADmnGwdRJZMzNmBdBLMoG9x_F_wFiZWteQ</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Lu, Xin</creator><creator>Zeng, Mei</creator><creator>Xu, Jialiang</creator><creator>Zhou, Haijian</creator><creator>Gu, Baoke</creator><creator>Li, Zhenpeng</creator><creator>Jin, Huiming</creator><creator>Wang, Xiaoxun</creator><creator>Zhang, Wen</creator><creator>Hu, Yongfei</creator><creator>Xiao, Wenjia</creator><creator>Zhu, Baoli</creator><creator>Xu, Xuebin</creator><creator>Kan, Biao</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190401</creationdate><title>Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016</title><author>Lu, Xin ; Zeng, Mei ; Xu, Jialiang ; Zhou, Haijian ; Gu, Baoke ; Li, Zhenpeng ; Jin, Huiming ; Wang, Xiaoxun ; Zhang, Wen ; Hu, Yongfei ; Xiao, Wenjia ; Zhu, Baoli ; Xu, Xuebin ; Kan, Biao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-9e9c2aa442cc44f211d26197693bf37ad2969978e6aff62d7b74fe14b3e28d593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>China - epidemiology</topic><topic>Diarrhea - epidemiology</topic><topic>Diarrhea - history</topic><topic>Diarrhea - microbiology</topic><topic>Female</topic><topic>Genes, Bacterial</topic><topic>Genome, Bacterial</topic><topic>Genomics - methods</topic><topic>History, 21st Century</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>mcr-1</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Typing</topic><topic>Multi-drug resistant</topic><topic>Outbreak</topic><topic>Outpatients</topic><topic>Phylogeny</topic><topic>Plasmids - genetics</topic><topic>Public Health Surveillance</topic><topic>Research paper</topic><topic>Salmonella</topic><topic>Salmonella - classification</topic><topic>Salmonella - drug effects</topic><topic>Salmonella - genetics</topic><topic>Salmonella Infections - epidemiology</topic><topic>Salmonella Infections - history</topic><topic>Salmonella Infections - microbiology</topic><topic>Serogroup</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Xin</creatorcontrib><creatorcontrib>Zeng, Mei</creatorcontrib><creatorcontrib>Xu, Jialiang</creatorcontrib><creatorcontrib>Zhou, Haijian</creatorcontrib><creatorcontrib>Gu, Baoke</creatorcontrib><creatorcontrib>Li, Zhenpeng</creatorcontrib><creatorcontrib>Jin, Huiming</creatorcontrib><creatorcontrib>Wang, Xiaoxun</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Hu, Yongfei</creatorcontrib><creatorcontrib>Xiao, Wenjia</creatorcontrib><creatorcontrib>Zhu, Baoli</creatorcontrib><creatorcontrib>Xu, Xuebin</creatorcontrib><creatorcontrib>Kan, Biao</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>EBioMedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Xin</au><au>Zeng, Mei</au><au>Xu, Jialiang</au><au>Zhou, Haijian</au><au>Gu, Baoke</au><au>Li, Zhenpeng</au><au>Jin, Huiming</au><au>Wang, Xiaoxun</au><au>Zhang, Wen</au><au>Hu, Yongfei</au><au>Xiao, Wenjia</au><au>Zhu, Baoli</au><au>Xu, Xuebin</au><au>Kan, Biao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016</atitle><jtitle>EBioMedicine</jtitle><addtitle>EBioMedicine</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>42</volume><spage>133</spage><epage>144</epage><pages>133-144</pages><issn>2352-3964</issn><eissn>2352-3964</eissn><abstract>Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated.
This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes.
Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5 years. All strains, including fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing strains, were multi-drug resistant. S. Typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. Phylogeny based on the genomes combined with complete plasmid sequences revealed some clusters, suggesting the presence of mcr-1-harbouring Salmonella outbreaks in the community. Most mcr-1-positive strains were clustered together with the pork strains, strongly suggesting pork consumption as a main infection source.
The mcr-1-harbouring Salmonella prevalence in community-acquired diarrhoea displays a rapid increase trend, and the ESBL-mcr-1-harbouring Salmonella poses a threat for children. These findings highlight the necessary and significance of prohibiting colistin use in animals and continuous monitoring of mcr-1-harbouring Salmonella.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30905850</pmid><doi>10.1016/j.ebiom.2019.03.006</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Child Child, Preschool Children China - epidemiology Diarrhea - epidemiology Diarrhea - history Diarrhea - microbiology Female Genes, Bacterial Genome, Bacterial Genomics - methods History, 21st Century Humans Infant Male mcr-1 Microbial Sensitivity Tests Molecular Typing Multi-drug resistant Outbreak Outpatients Phylogeny Plasmids - genetics Public Health Surveillance Research paper Salmonella Salmonella - classification Salmonella - drug effects Salmonella - genetics Salmonella Infections - epidemiology Salmonella Infections - history Salmonella Infections - microbiology Serogroup Swine |
| title | Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016 |
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