Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis
The aim of the present study was to investigate the expression of microRNA (miRNA/miR)-214 in the sera of elderly patients with acute myocardial infarction (AMI) and the mechanism of how its expression affects cardiomyocyte apoptosis in these patients. The expression levels of miRNA-214 in elderly p...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2019-06, Vol.17 (6), p.4657-4662 |
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| description | The aim of the present study was to investigate the expression of microRNA (miRNA/miR)-214 in the sera of elderly patients with acute myocardial infarction (AMI) and the mechanism of how its expression affects cardiomyocyte apoptosis in these patients. The expression levels of miRNA-214 in elderly patients with AMI, unstable angina (UA) and healthy elderly subjects were detected by reverse transcription-quantitative polymerase chain reaction, and the correlation between the relative expressions of sera miRNA-214 and myocardial enzymes in elderly patients with AMI was examined by Pearson's analysis. Human cardiomyocyte (HCM) cell lines with a high expression miRNA-214 were established. The apoptotic rates of the different groups of cells were detected by flow cytometry and TUNEL assay. The expression of miRNA-214 target genes in the different groups of cells was detected by western blot assay. The relative expression levels of sera miR-214 in elderly AMI patients, UA patients and healthy subjects (as determined by physical examination) were 15.79±4.66, 4.60±2.51 and 2.07±0.99, respectively. The differences between each group were statistically significant (P |
| doi_str_mv | 10.3892/etm.2019.7464 |
| format | Article |
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O
induction, and the rate of apoptosis decreased by 63.64, 21.95, 46.67 and 50.05%, respectively. miRNA-214 was highly expressed in the sera of elderly patients with AMI, which may inhibit myocardial cell apoptosis by inhibiting the expression of miR-214 target genes including
.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2019.7464</identifier><identifier>PMID: 31086597</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Angina pectoris ; Anopheles ; Apoptosis ; Aspartate ; Body mass index ; Cardiomyocytes ; Cardiovascular disease ; Cholesterol ; Creatine ; Creatine kinase ; Diagnosis ; EDTA ; Elderly patients ; Enzymes ; Gene expression ; Genes ; Genetic aspects ; Health aspects ; Heart attack ; Heart attacks ; Hospitals ; Ischemia ; Lymphomas ; MicroRNA ; Mortality ; Older people ; Phosphatases ; Polymerase chain reaction ; Proteins ; Studies ; Troponin ; Tumor proteins ; Tumors ; Unstable angina</subject><ispartof>Experimental and therapeutic medicine, 2019-06, Vol.17 (6), p.4657-4662</ispartof><rights>COPYRIGHT 2019 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2019</rights><rights>Copyright © 2019, Spandidos Publications 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8fb7b2937badb93476a5410b4943bd6057aad65e7fb73c78f7cf01999701e3273</citedby><cites>FETCH-LOGICAL-c412t-8fb7b2937badb93476a5410b4943bd6057aad65e7fb73c78f7cf01999701e3273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489010/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489010/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31086597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Yugang</creatorcontrib><creatorcontrib>Lv, Lei</creatorcontrib><creatorcontrib>Wang, Wenni</creatorcontrib><title>Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The aim of the present study was to investigate the expression of microRNA (miRNA/miR)-214 in the sera of elderly patients with acute myocardial infarction (AMI) and the mechanism of how its expression affects cardiomyocyte apoptosis in these patients. The expression levels of miRNA-214 in elderly patients with AMI, unstable angina (UA) and healthy elderly subjects were detected by reverse transcription-quantitative polymerase chain reaction, and the correlation between the relative expressions of sera miRNA-214 and myocardial enzymes in elderly patients with AMI was examined by Pearson's analysis. Human cardiomyocyte (HCM) cell lines with a high expression miRNA-214 were established. The apoptotic rates of the different groups of cells were detected by flow cytometry and TUNEL assay. The expression of miRNA-214 target genes in the different groups of cells was detected by western blot assay. The relative expression levels of sera miR-214 in elderly AMI patients, UA patients and healthy subjects (as determined by physical examination) were 15.79±4.66, 4.60±2.51 and 2.07±0.99, respectively. The differences between each group were statistically significant (P<0.05). The relative expression of sera miRNA-214 in elderly AMI patients was positively correlated with sera aspartate aminotransferase (r=0.361, P=0.0174), lactate dehydrogenase (r=0.425, P=0.0045), creatine kinase-MB (r=0.835, P<0.001) and cardiac troponin (r=0.770, P<0.001). When compared with normal HCMs, the expressions of p53-upregulated modulator of apoptosis (PUMA), phosphatase and tensin homolog (PTEN), B-cell lymphoma-2-associated X protein (Bax) and caspase 7 proteins was decreased in HCMs overexpressing miRNA-214 following H
O
induction, and the rate of apoptosis decreased by 63.64, 21.95, 46.67 and 50.05%, respectively. miRNA-214 was highly expressed in the sera of elderly patients with AMI, which may inhibit myocardial cell apoptosis by inhibiting the expression of miR-214 target genes including
.</description><subject>Angina pectoris</subject><subject>Anopheles</subject><subject>Apoptosis</subject><subject>Aspartate</subject><subject>Body mass index</subject><subject>Cardiomyocytes</subject><subject>Cardiovascular disease</subject><subject>Cholesterol</subject><subject>Creatine</subject><subject>Creatine kinase</subject><subject>Diagnosis</subject><subject>EDTA</subject><subject>Elderly patients</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Heart attack</subject><subject>Heart attacks</subject><subject>Hospitals</subject><subject>Ischemia</subject><subject>Lymphomas</subject><subject>MicroRNA</subject><subject>Mortality</subject><subject>Older people</subject><subject>Phosphatases</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Studies</subject><subject>Troponin</subject><subject>Tumor proteins</subject><subject>Tumors</subject><subject>Unstable angina</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkstrFTEUxgdRbKldupWAGzdzzWvy2AiXUh9QFETXIZM56U2ZmYxJrnq3_uVm7LW0YrJIOPl9XziHr2meE7xhStPXUKYNxURvJBf8UXNKpKYtwaR7fLxjrchJc57zDa6rE0Sp7mlzwghWotPytPl1-XNJkHOIM4oeTeHzx21LCUdhRmUHKEOy6wOMA6TxgBZbAswlox-h7JB1-wJoOkRn0xDsWFXeJldWNzsPKFQQvAdXUK38geJKH6rKLnEpMYf8rHni7Zjh_HieNV_fXn65eN9efXr34WJ71TpOaGmV72VPNZO9HXrNuBS24wT3XHPWDwJ30tpBdCArx5xUXjpfR6O1xAQYleyseXPru-z7CQZX20h2NEsKk00HE20wD1_msDPX8bsRXGlMcDV4dTRI8dsecjFTyA7G0c4Q99lQyohWSkpR0Zf_oDdxn-baXqUo40IIfo-6tiOYOrtY_3Wrqdl2SilW3bpKbf5D1T3AFFycwYdafyBobwUuxZwT-LseCTZrbkzNjVlzY9bcVP7F_cHc0X9Twn4DBS6-ag</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Yin, Yugang</creator><creator>Lv, Lei</creator><creator>Wang, Wenni</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190601</creationdate><title>Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis</title><author>Yin, Yugang ; Lv, Lei ; Wang, Wenni</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8fb7b2937badb93476a5410b4943bd6057aad65e7fb73c78f7cf01999701e3273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angina pectoris</topic><topic>Anopheles</topic><topic>Apoptosis</topic><topic>Aspartate</topic><topic>Body mass index</topic><topic>Cardiomyocytes</topic><topic>Cardiovascular disease</topic><topic>Cholesterol</topic><topic>Creatine</topic><topic>Creatine kinase</topic><topic>Diagnosis</topic><topic>EDTA</topic><topic>Elderly patients</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Heart attack</topic><topic>Heart attacks</topic><topic>Hospitals</topic><topic>Ischemia</topic><topic>Lymphomas</topic><topic>MicroRNA</topic><topic>Mortality</topic><topic>Older people</topic><topic>Phosphatases</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Studies</topic><topic>Troponin</topic><topic>Tumor proteins</topic><topic>Tumors</topic><topic>Unstable angina</topic><toplevel>online_resources</toplevel><creatorcontrib>Yin, Yugang</creatorcontrib><creatorcontrib>Lv, Lei</creatorcontrib><creatorcontrib>Wang, Wenni</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Yugang</au><au>Lv, Lei</au><au>Wang, Wenni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>17</volume><issue>6</issue><spage>4657</spage><epage>4662</epage><pages>4657-4662</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The aim of the present study was to investigate the expression of microRNA (miRNA/miR)-214 in the sera of elderly patients with acute myocardial infarction (AMI) and the mechanism of how its expression affects cardiomyocyte apoptosis in these patients. The expression levels of miRNA-214 in elderly patients with AMI, unstable angina (UA) and healthy elderly subjects were detected by reverse transcription-quantitative polymerase chain reaction, and the correlation between the relative expressions of sera miRNA-214 and myocardial enzymes in elderly patients with AMI was examined by Pearson's analysis. Human cardiomyocyte (HCM) cell lines with a high expression miRNA-214 were established. The apoptotic rates of the different groups of cells were detected by flow cytometry and TUNEL assay. The expression of miRNA-214 target genes in the different groups of cells was detected by western blot assay. The relative expression levels of sera miR-214 in elderly AMI patients, UA patients and healthy subjects (as determined by physical examination) were 15.79±4.66, 4.60±2.51 and 2.07±0.99, respectively. The differences between each group were statistically significant (P<0.05). The relative expression of sera miRNA-214 in elderly AMI patients was positively correlated with sera aspartate aminotransferase (r=0.361, P=0.0174), lactate dehydrogenase (r=0.425, P=0.0045), creatine kinase-MB (r=0.835, P<0.001) and cardiac troponin (r=0.770, P<0.001). When compared with normal HCMs, the expressions of p53-upregulated modulator of apoptosis (PUMA), phosphatase and tensin homolog (PTEN), B-cell lymphoma-2-associated X protein (Bax) and caspase 7 proteins was decreased in HCMs overexpressing miRNA-214 following H
O
induction, and the rate of apoptosis decreased by 63.64, 21.95, 46.67 and 50.05%, respectively. miRNA-214 was highly expressed in the sera of elderly patients with AMI, which may inhibit myocardial cell apoptosis by inhibiting the expression of miR-214 target genes including
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| subjects | Angina pectoris Anopheles Apoptosis Aspartate Body mass index Cardiomyocytes Cardiovascular disease Cholesterol Creatine Creatine kinase Diagnosis EDTA Elderly patients Enzymes Gene expression Genes Genetic aspects Health aspects Heart attack Heart attacks Hospitals Ischemia Lymphomas MicroRNA Mortality Older people Phosphatases Polymerase chain reaction Proteins Studies Troponin Tumor proteins Tumors Unstable angina |
| title | Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis |
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