Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals

A family of proteases called caspases mediate apoptosis signaling in animals. We report a GFP-based fluorogenic protease reporter, dubbed “FlipGFP”, by flipping a beta strand of the GFP. Upon protease activation and cleavage, the beta strand is restored, leading to reconstitution of the GFP and fluo...

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Veröffentlicht in:	Journal of the American Chemical Society 2019-03, Vol.141 (11), p.4526-4530
Hauptverfasser:	Zhang, Qiang, Schepis, Antonino, Huang, Hai, Yang, Junjiao, Ma, Wen, Torra, Joaquim, Zhang, Shao-Qing, Yang, Lina, Wu, Haifan, Nonell, Santi, Dong, Zhiqiang, Kornberg, Thomas B, Coughlin, Shaun R, Shu, Xiaokun
Format:	Artikel
Sprache:	eng
Schlagworte:	animal development Animals Apoptosis caspases Danio rerio Drosophila Drosophila melanogaster embryo (animal) engineering fluorescence Genes, Reporter - genetics Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics HEK293 Cells HeLa Cells Humans image analysis midgut Molecular Imaging monitoring Peptide Hydrolases - chemistry Peptide Hydrolases - genetics Protein Conformation, beta-Strand red fluorescent protein reporter genes
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