Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA
Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome ca...
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| Veröffentlicht in: | International journal of molecular sciences 2019-03, Vol.20 (7), p.1585 |
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| container_title | International journal of molecular sciences |
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| creator | Dubrovina, Alexandra S Aleynova, Olga A Kalachev, Alexander V Suprun, Andrey R Ogneva, Zlata V Kiselev, Konstantin V |
| description | Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in
genome by direct exogenous application of target long dsRNAs. The data revealed that
synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (
) or neomycin phosphotransferase II (
) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies. |
| doi_str_mv | 10.3390/ijms20071585 |
| format | Article |
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genome by direct exogenous application of target long dsRNAs. The data revealed that
synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (
) or neomycin phosphotransferase II (
) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20071585</identifier><identifier>PMID: 30934883</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Cultivation ; Double-stranded RNA ; Gene expression ; Genes ; Investigations ; MicroRNAs ; Microscopy ; Proteins ; RNA polymerase ; RNA-mediated interference ; siRNA ; Transcription ; Transgenes ; Transgenic plants</subject><ispartof>International journal of molecular sciences, 2019-03, Vol.20 (7), p.1585</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-15bcd70a49e63b8ddf40f55cc71b7200a6b1a92d578a8efe06c3c9d2cfd680a13</citedby><cites>FETCH-LOGICAL-c478t-15bcd70a49e63b8ddf40f55cc71b7200a6b1a92d578a8efe06c3c9d2cfd680a13</cites><orcidid>0000-0003-2392-3636 ; 0000-0002-2549-8568 ; 0000-0002-0516-6144</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479969/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479969/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30934883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dubrovina, Alexandra S</creatorcontrib><creatorcontrib>Aleynova, Olga A</creatorcontrib><creatorcontrib>Kalachev, Alexander V</creatorcontrib><creatorcontrib>Suprun, Andrey R</creatorcontrib><creatorcontrib>Ogneva, Zlata V</creatorcontrib><creatorcontrib>Kiselev, Konstantin V</creatorcontrib><title>Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in
genome by direct exogenous application of target long dsRNAs. The data revealed that
synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (
) or neomycin phosphotransferase II (
) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.</description><subject>Cultivation</subject><subject>Double-stranded RNA</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Investigations</subject><subject>MicroRNAs</subject><subject>Microscopy</subject><subject>Proteins</subject><subject>RNA polymerase</subject><subject>RNA-mediated interference</subject><subject>siRNA</subject><subject>Transcription</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkd1LwzAUxYMoTqdvPkvBFx-s3ib9yoswxtTBUHETfAtpkm4ZXVqTdrj_3o59MIULuXB-OeTkIHQVwD0hFB70fOEwQBJEaXSEzoIQYx8gTo4P9g46d24OgAmO6CnqEKAkTFNyhr6GRjai1qXxytybWG7cVBnljZuqssq5taCN915wUztvqbk3-KmVNbzwelVVaMF3d8crU89UrYUn3cdr7wKd5Lxw6nJ7dtHn02DSf_FHb8_Dfm_kizBJaz-IMiET4CFVMclSKfMQ8igSIgmypI3F4yzgFMsoSXmqcgWxIIJKLHIZp8AD0kWPG9-qyRZKCmVqywtWWb3gdsVKrtlfxegZm5ZLFocJpTFtDW63Brb8bpSr2UI7oYo2sSobxzCGdgCH0KI3_9B52az_oqUIwTgilMQtdbehhC2dsyrfPyYAtq6MHVbW4teHAfbwriPyCz5Qk4I</recordid><startdate>20190329</startdate><enddate>20190329</enddate><creator>Dubrovina, Alexandra S</creator><creator>Aleynova, Olga A</creator><creator>Kalachev, Alexander V</creator><creator>Suprun, Andrey R</creator><creator>Ogneva, Zlata V</creator><creator>Kiselev, Konstantin V</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2392-3636</orcidid><orcidid>https://orcid.org/0000-0002-2549-8568</orcidid><orcidid>https://orcid.org/0000-0002-0516-6144</orcidid></search><sort><creationdate>20190329</creationdate><title>Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA</title><author>Dubrovina, Alexandra S ; 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genome by direct exogenous application of target long dsRNAs. The data revealed that
synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (
) or neomycin phosphotransferase II (
) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>30934883</pmid><doi>10.3390/ijms20071585</doi><orcidid>https://orcid.org/0000-0003-2392-3636</orcidid><orcidid>https://orcid.org/0000-0002-2549-8568</orcidid><orcidid>https://orcid.org/0000-0002-0516-6144</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cultivation Double-stranded RNA Gene expression Genes Investigations MicroRNAs Microscopy Proteins RNA polymerase RNA-mediated interference siRNA Transcription Transgenes Transgenic plants |
| title | Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA |
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