A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products

An efficient PCR cloning method is indispensable in modern molecular biology, as it can greatly improve the efficiency of DNA cloning processes. Here, I describe the development of three vectors for TA cloning and blunt-end cloning. Specifically, pCRT and pCRZeroT were designed to improve the effici...

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Veröffentlicht in:	Scientific reports 2019-04, Vol.9 (1), p.6417-6417, Article 6417
1. Verfasser:	Motohashi, Ken
Format:	Artikel
Sprache:	eng
Schlagworte:	38/44 38/77 631/1647/1511 631/1647/1513/2216 Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Cloning Cloning vectors Cloning, Molecular - methods DNA Primers - chemistry DNA Primers - metabolism Efficiency Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genetic Engineering - methods Humanities and Social Sciences Humans Molecular biology multidisciplinary Plasmids Plasmids - chemistry Plasmids - metabolism Polymerase chain reaction Polymerase Chain Reaction - methods Science Science (multidisciplinary)
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