Analysis of discrete local variability and structural covariance in macromolecular assemblies using Cryo-EM and focused classification

•Quantitative evaluation of two single-particle cryo-EM classification methods implemented in the cisTEM and Frealign image processing packages•Detection of discrete states and structural covariance in macromolecular complexes•Classification of a small flexible RNA segment attached to a ribosomal la...

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Veröffentlicht in:	Ultramicroscopy 2019-08, Vol.203, p.170-180
Hauptverfasser:	Zhang, Cheng, Cantara, William, Jeon, Youngmin, Musier-Forsyth, Karin, Grigorieff, Nikolaus, Lyumkis, Dmitry
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - chemistry cisTEM Classification Cryoelectron Microscopy - methods Frealign Heterogeneity Image Processing, Computer-Assisted - methods Macromolecular Substances - chemistry Ribosome Ribosome Subunits, Large - chemistry RNA - chemistry Single-particle cryo-EM
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