Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming

The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In...

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Veröffentlicht in:Journal of the American Chemical Society 2019-01, Vol.141 (1), p.262-271
Hauptverfasser: Marín, María del Carmen, Agathangelou, Damianos, Orozco-Gonzalez, Yoelvis, Valentini, Alessio, Kato, Yoshitaka, Abe-Yoshizumi, Rei, Kandori, Hideki, Choi, Ahreum, Jung, Kwang-Hwan, Haacke, Stefan, Olivucci, Massimo
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container_end_page 271
container_issue 1
container_start_page 262
container_title Journal of the American Chemical Society
container_volume 141
creator Marín, María del Carmen
Agathangelou, Damianos
Orozco-Gonzalez, Yoelvis
Valentini, Alessio
Kato, Yoshitaka
Abe-Yoshizumi, Rei
Kandori, Hideki
Choi, Ahreum
Jung, Kwang-Hwan
Haacke, Stefan
Olivucci, Massimo
description The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.
doi_str_mv 10.1021/jacs.8b09311
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Micro and nanotechnologies
Microelectronics
title Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming
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