Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes
Radopholus similis is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of R. similis . In this study, 64761 unigenes were generated from eggs, juveniles, females a...
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| Veröffentlicht in: | Scientific reports 2019-04, Vol.9 (1), p.6277-6277, Article 6277 |
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| creator | Huang, Xin Xu, Chun-Ling Yang, Si-Hua Li, Jun-Yi Wang, Hong-Le Zhang, Zi-Xu Chen, Chun Xie, Hui |
| description | Radopholus similis
is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of
R. similis
. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of
R. similis
. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in
R. similis
, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on
R. similis
. |
| doi_str_mv | 10.1038/s41598-019-42724-7 |
| format | Article |
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is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of
R. similis
. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of
R. similis
. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in
R. similis
, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on
R. similis
.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-42724-7</identifier><identifier>PMID: 31000750</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/77 ; 45/91 ; 631/208/199 ; 631/326/417 ; Animals ; Cell walls ; Developmental stages ; Eggs ; Endoparasites ; Gene expression ; Gene Expression Regulation ; Helminth Proteins - genetics ; Humanities and Social Sciences ; Molecular modelling ; multidisciplinary ; Nematodes ; Parasitism ; Phylogeny ; Plant Diseases - genetics ; Plant Diseases - parasitology ; Plants - genetics ; Plants - parasitology ; Radopholus similis ; Science ; Science (multidisciplinary) ; Transcriptome - genetics ; Tylenchoidea - genetics ; Tylenchoidea - pathogenicity</subject><ispartof>Scientific reports, 2019-04, Vol.9 (1), p.6277-6277, Article 6277</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-556d202120a8674a98b4f37a0a019a9f03862ed30af9d41920a55cf3311ec50b3</citedby><cites>FETCH-LOGICAL-c511t-556d202120a8674a98b4f37a0a019a9f03862ed30af9d41920a55cf3311ec50b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472380/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472380/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,27911,27912,41107,42176,51563,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31000750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Xin</creatorcontrib><creatorcontrib>Xu, Chun-Ling</creatorcontrib><creatorcontrib>Yang, Si-Hua</creatorcontrib><creatorcontrib>Li, Jun-Yi</creatorcontrib><creatorcontrib>Wang, Hong-Le</creatorcontrib><creatorcontrib>Zhang, Zi-Xu</creatorcontrib><creatorcontrib>Chen, Chun</creatorcontrib><creatorcontrib>Xie, Hui</creatorcontrib><title>Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Radopholus similis
is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of
R. similis
. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of
R. similis
. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in
R. similis
, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on
R. similis
.</description><subject>45/77</subject><subject>45/91</subject><subject>631/208/199</subject><subject>631/326/417</subject><subject>Animals</subject><subject>Cell walls</subject><subject>Developmental stages</subject><subject>Eggs</subject><subject>Endoparasites</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Helminth Proteins - genetics</subject><subject>Humanities and Social Sciences</subject><subject>Molecular modelling</subject><subject>multidisciplinary</subject><subject>Nematodes</subject><subject>Parasitism</subject><subject>Phylogeny</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - parasitology</subject><subject>Plants - genetics</subject><subject>Plants - parasitology</subject><subject>Radopholus similis</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transcriptome - genetics</subject><subject>Tylenchoidea - genetics</subject><subject>Tylenchoidea - pathogenicity</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kstu1TAQhiMEolXpC7BAltiwIOBLnMsGCVXcpCMhIVhbc5xx6iqJg-0gncfiDZk2bU9hgTe2Nd_8_8xoiuK54G8EV-3bVAndtSUXXVnJRlZl86g4lbzSpVRSPn7wPinOU7ridLTsKtE9LU6UoF-j-Wnxe-cdlinDgCwtaL3zluUIc7LRLzlMmFhwDNjkhwg5xAPDuQ8LREg-E7uMMGc240TBHl-zb0DRyzCuiSU_-dEnhuNqfQ8ZSab3zmFESjlKwNyzkcpgiYwzDodrx033CN05pGfFEwdjwvPb-6z48fHD94vP5e7rpy8X73el1ULkUuu6l1wKyaGtmwq6dl851QAHGhl0jmZYS-wVB9f1NBXitLZOKSHQar5XZ8W7TXdZ9xP2lmqOMJol-gniwQTw5u_I7C_NEH6ZumqkajkJvLoViOHniimbySeLIzWGYU1GSiG6qhZNTejLf9CrsMaZ2ruhlOxaWRElN8rGkFJEd1-M4OZ6Kcy2FIY6NDdLYRpKevGwjfuUuxUgQG1AotA8YDx6_0f2D7q-xqo</recordid><startdate>20190418</startdate><enddate>20190418</enddate><creator>Huang, Xin</creator><creator>Xu, Chun-Ling</creator><creator>Yang, Si-Hua</creator><creator>Li, Jun-Yi</creator><creator>Wang, Hong-Le</creator><creator>Zhang, Zi-Xu</creator><creator>Chen, Chun</creator><creator>Xie, Hui</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190418</creationdate><title>Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes</title><author>Huang, Xin ; 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is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of
R. similis
. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of
R. similis
. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in
R. similis
, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on
R. similis
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31000750</pmid><doi>10.1038/s41598-019-42724-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 45/77 45/91 631/208/199 631/326/417 Animals Cell walls Developmental stages Eggs Endoparasites Gene expression Gene Expression Regulation Helminth Proteins - genetics Humanities and Social Sciences Molecular modelling multidisciplinary Nematodes Parasitism Phylogeny Plant Diseases - genetics Plant Diseases - parasitology Plants - genetics Plants - parasitology Radopholus similis Science Science (multidisciplinary) Transcriptome - genetics Tylenchoidea - genetics Tylenchoidea - pathogenicity |
| title | Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes |
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