Extracellular matrix signaling activates differentiation of adult ovary-derived oogonial stem cells in a species-specific manner

To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner. Animal and human study. Research laboratory. Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice. Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and human ovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor. Gene expression and in vitro derived (IVD) oocyte formation. Culture of mOSCs on a collagen-based ECM significantly elevated the rate of differentiation of the cells into IVD oocytes. Mouse OSCs expressed many integrins, including Arg-Gly-Asp (RGD)–binding subunits, and ECM-mediated increases in mOSC differentiation were blocked by addition of integrin-antagonizing RGD peptides. In comparison, hOSCs expressed a different pattern of integrin subunits compared with mOSCs, and hOSCs were unresponsive to a collagen-based ECM; however, hOSCs exhibited increased differentiation into IVD oocytes when cultured on laminin. These data, along with in silico analysis of ECM protein profiles in human ovaries, indicate that ovarian ECM-based niche components function in a species-specific manner to control OSC differentiation. La señalización de la matriz extracelular activa la diferenciación en células madre derivadas de oogonias del ovario de una forma específica en cada especie. comprobar si las señales microambientales del ovario afectan la función de las células madre oogoniales (OSC) de una manera específica en cada especie. estudio animal y humano. laboratorio de investigación. células ováricas humanas obtenidas de tejido cortical ovárico crioconservado de mujeres en edad reproductiva, y células y tejidos ováricos de ratones hembra C57BL/6. se analizaron tejidos de ovario de ratón, OSC de ratón (mOSCs) y OSC humanas (hOSC) para determinar la expresión de las proteíanas en la matriz extracelular (MEC), y las OSC aisladas de ratones adultos y ovarios humanos se cultivaron en ausencia o presencia de proteínas MEC en ausencia o presencia de un inhibidor de señalización de integrinas. expresión génica y formación de ovocitos derivada in vitro (IVD). el cultivo de mOSCs en MEC de colágeno elevó significativamente la tasa de diferenciación de las células en ovocitos IVD. Las OSC