Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901
BACKGROUND Cisplatin-resistant gastric cancer (GC) occurs in patients with GC treated with cisplatin-based chemotherapy, which results in disease progression and early recurrence during the treatment. MATERIAL AND METHODS To understand the initiation and developmental mechanism underlying cisplatin-...
Gespeichert in:
| Veröffentlicht in: | Medical science monitor 2019-04, Vol.25, p.2386-2396 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2396 |
|---|---|
| container_issue | |
| container_start_page | 2386 |
| container_title | Medical science monitor |
| container_volume | 25 |
| creator | Deng, Zhenwei Wang, Huaiming Guo, Guohu Li, Xiyao Cai, Yongchang Tang, Yuxin Wang, Yijun Li, Jiabao Lu, Zhibin Yu, Xueqiao Li, Ruiping Li, Libo |
| description | BACKGROUND Cisplatin-resistant gastric cancer (GC) occurs in patients with GC treated with cisplatin-based chemotherapy, which results in disease progression and early recurrence during the treatment. MATERIAL AND METHODS To understand the initiation and developmental mechanism underlying cisplatin-resistant GC, we developed cisplatin-resistant SGC7901 cells (SGC7901/DDP) from the parental cells (SGC7901/S) by continuous exposure to increasing concentrations of cisplatin and subjected these 2 cell lines to RNA sequencing analysis. The data were verified by quantitative polymerase chain reaction and their functional role was evaluated by cell counting kit 8 assay and cell apoptosis and cell cycle flow cytometric analysis. Bioinformatics analysis was performed to classify the differentially-expressed genes (DEGs) involved in the development of cisplatin resistance. RESULTS In comparison with SGC7901/S cells, SGC7901/DDP cells showed a total of 3165 DEGs (2014 upregulated and 1151 downregulated, fold change ≥2, and adjusted P value |
| doi_str_mv | 10.12659/MSM.915866 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6457324</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>30938333</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-291bf33a6976738409afa0efe14141de1ddfcdfac94f9ffff1d74edb3eca96483</originalsourceid><addsrcrecordid>eNpVkNFLwzAQxoMoTqdPvkvepTNp0rR5EUbRKmxTNn0uWXqZ0S6dSSfuv7c4HfPu4Q7u-76DH0IXlAxoLBJ5PZ6NB5ImmRAH6IQKziKWJuRwb--h0xDeCIkzQZJj1GNEsowxdoLeJ_DVRgU48Kq1jcMz-FiD09Yt8NCpehNswI3By-lkiJ98Y2wN2Dqc27CqO4eLptBJWuVaXKjQeqtxrpwGj3OoazyyDvCsyFNJ6Bk6MqoOcP47--jl7vY5v49Gj8VDPhxFmsesjWJJ54YxJWQqUpZxIpVRBAxQ3nUFtKqMrozSkhtpuqJVyqGaM9BKCp6xPrrZ5q7W8yVUGlzrVV2uvF0qvykbZcv_F2dfy0XzWQqepCzmXcDVNkD7JgQPZuelpPxhXnbMyy3zTn25_26n_YPMvgFzxH62</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Deng, Zhenwei ; Wang, Huaiming ; Guo, Guohu ; Li, Xiyao ; Cai, Yongchang ; Tang, Yuxin ; Wang, Yijun ; Li, Jiabao ; Lu, Zhibin ; Yu, Xueqiao ; Li, Ruiping ; Li, Libo</creator><creatorcontrib>Deng, Zhenwei ; Wang, Huaiming ; Guo, Guohu ; Li, Xiyao ; Cai, Yongchang ; Tang, Yuxin ; Wang, Yijun ; Li, Jiabao ; Lu, Zhibin ; Yu, Xueqiao ; Li, Ruiping ; Li, Libo</creatorcontrib><description>BACKGROUND Cisplatin-resistant gastric cancer (GC) occurs in patients with GC treated with cisplatin-based chemotherapy, which results in disease progression and early recurrence during the treatment. MATERIAL AND METHODS To understand the initiation and developmental mechanism underlying cisplatin-resistant GC, we developed cisplatin-resistant SGC7901 cells (SGC7901/DDP) from the parental cells (SGC7901/S) by continuous exposure to increasing concentrations of cisplatin and subjected these 2 cell lines to RNA sequencing analysis. The data were verified by quantitative polymerase chain reaction and their functional role was evaluated by cell counting kit 8 assay and cell apoptosis and cell cycle flow cytometric analysis. Bioinformatics analysis was performed to classify the differentially-expressed genes (DEGs) involved in the development of cisplatin resistance. RESULTS In comparison with SGC7901/S cells, SGC7901/DDP cells showed a total of 3165 DEGs (2014 upregulated and 1151 downregulated, fold change ≥2, and adjusted P value <0.001). qRT-PCR confirmed the reliability of the RNA sequencing results. Depletion of the top 5 upregulated mRNAs reversed the resistant index, increased apoptotic SGC7901/DDP cells, and arrested the cells at G2/M phase. Gene ontology analysis revealed that the DEGs mainly regulate metabolic process, immune system, locomotion, cell adhesion, cell growth, cell death, cytoskeleton organization, cell binding, signal transducing activity, and antioxidant activity. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEGs were mainly involved in the PI3K-Akt signaling pathway, Rap1 signaling pathway, proteoglycans in cancer, regulation of actin cytoskeleton, and pathways in cancer. CONCLUSIONS The present study is the first to interrogate mRNAs profiles in human GC cells with cisplatin resistance using RNA sequencing, which may assist in discovering potential therapeutic targets for cisplatin-resistant GC patients.</description><identifier>ISSN: 1643-3750</identifier><identifier>ISSN: 1234-1010</identifier><identifier>EISSN: 1643-3750</identifier><identifier>DOI: 10.12659/MSM.915866</identifier><identifier>PMID: 30938333</identifier><language>eng</language><publisher>United States: International Scientific Literature, Inc</publisher><subject>Lab/In Vitro Research</subject><ispartof>Medical science monitor, 2019-04, Vol.25, p.2386-2396</ispartof><rights>Med Sci Monit, 2019 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-291bf33a6976738409afa0efe14141de1ddfcdfac94f9ffff1d74edb3eca96483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457324/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457324/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30938333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Zhenwei</creatorcontrib><creatorcontrib>Wang, Huaiming</creatorcontrib><creatorcontrib>Guo, Guohu</creatorcontrib><creatorcontrib>Li, Xiyao</creatorcontrib><creatorcontrib>Cai, Yongchang</creatorcontrib><creatorcontrib>Tang, Yuxin</creatorcontrib><creatorcontrib>Wang, Yijun</creatorcontrib><creatorcontrib>Li, Jiabao</creatorcontrib><creatorcontrib>Lu, Zhibin</creatorcontrib><creatorcontrib>Yu, Xueqiao</creatorcontrib><creatorcontrib>Li, Ruiping</creatorcontrib><creatorcontrib>Li, Libo</creatorcontrib><title>Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901</title><title>Medical science monitor</title><addtitle>Med Sci Monit</addtitle><description>BACKGROUND Cisplatin-resistant gastric cancer (GC) occurs in patients with GC treated with cisplatin-based chemotherapy, which results in disease progression and early recurrence during the treatment. MATERIAL AND METHODS To understand the initiation and developmental mechanism underlying cisplatin-resistant GC, we developed cisplatin-resistant SGC7901 cells (SGC7901/DDP) from the parental cells (SGC7901/S) by continuous exposure to increasing concentrations of cisplatin and subjected these 2 cell lines to RNA sequencing analysis. The data were verified by quantitative polymerase chain reaction and their functional role was evaluated by cell counting kit 8 assay and cell apoptosis and cell cycle flow cytometric analysis. Bioinformatics analysis was performed to classify the differentially-expressed genes (DEGs) involved in the development of cisplatin resistance. RESULTS In comparison with SGC7901/S cells, SGC7901/DDP cells showed a total of 3165 DEGs (2014 upregulated and 1151 downregulated, fold change ≥2, and adjusted P value <0.001). qRT-PCR confirmed the reliability of the RNA sequencing results. Depletion of the top 5 upregulated mRNAs reversed the resistant index, increased apoptotic SGC7901/DDP cells, and arrested the cells at G2/M phase. Gene ontology analysis revealed that the DEGs mainly regulate metabolic process, immune system, locomotion, cell adhesion, cell growth, cell death, cytoskeleton organization, cell binding, signal transducing activity, and antioxidant activity. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEGs were mainly involved in the PI3K-Akt signaling pathway, Rap1 signaling pathway, proteoglycans in cancer, regulation of actin cytoskeleton, and pathways in cancer. CONCLUSIONS The present study is the first to interrogate mRNAs profiles in human GC cells with cisplatin resistance using RNA sequencing, which may assist in discovering potential therapeutic targets for cisplatin-resistant GC patients.</description><subject>Lab/In Vitro Research</subject><issn>1643-3750</issn><issn>1234-1010</issn><issn>1643-3750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVkNFLwzAQxoMoTqdPvkvepTNp0rR5EUbRKmxTNn0uWXqZ0S6dSSfuv7c4HfPu4Q7u-76DH0IXlAxoLBJ5PZ6NB5ImmRAH6IQKziKWJuRwb--h0xDeCIkzQZJj1GNEsowxdoLeJ_DVRgU48Kq1jcMz-FiD09Yt8NCpehNswI3By-lkiJ98Y2wN2Dqc27CqO4eLptBJWuVaXKjQeqtxrpwGj3OoazyyDvCsyFNJ6Bk6MqoOcP47--jl7vY5v49Gj8VDPhxFmsesjWJJ54YxJWQqUpZxIpVRBAxQ3nUFtKqMrozSkhtpuqJVyqGaM9BKCp6xPrrZ5q7W8yVUGlzrVV2uvF0qvykbZcv_F2dfy0XzWQqepCzmXcDVNkD7JgQPZuelpPxhXnbMyy3zTn25_26n_YPMvgFzxH62</recordid><startdate>20190402</startdate><enddate>20190402</enddate><creator>Deng, Zhenwei</creator><creator>Wang, Huaiming</creator><creator>Guo, Guohu</creator><creator>Li, Xiyao</creator><creator>Cai, Yongchang</creator><creator>Tang, Yuxin</creator><creator>Wang, Yijun</creator><creator>Li, Jiabao</creator><creator>Lu, Zhibin</creator><creator>Yu, Xueqiao</creator><creator>Li, Ruiping</creator><creator>Li, Libo</creator><general>International Scientific Literature, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20190402</creationdate><title>Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901</title><author>Deng, Zhenwei ; Wang, Huaiming ; Guo, Guohu ; Li, Xiyao ; Cai, Yongchang ; Tang, Yuxin ; Wang, Yijun ; Li, Jiabao ; Lu, Zhibin ; Yu, Xueqiao ; Li, Ruiping ; Li, Libo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-291bf33a6976738409afa0efe14141de1ddfcdfac94f9ffff1d74edb3eca96483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Lab/In Vitro Research</topic><toplevel>online_resources</toplevel><creatorcontrib>Deng, Zhenwei</creatorcontrib><creatorcontrib>Wang, Huaiming</creatorcontrib><creatorcontrib>Guo, Guohu</creatorcontrib><creatorcontrib>Li, Xiyao</creatorcontrib><creatorcontrib>Cai, Yongchang</creatorcontrib><creatorcontrib>Tang, Yuxin</creatorcontrib><creatorcontrib>Wang, Yijun</creatorcontrib><creatorcontrib>Li, Jiabao</creatorcontrib><creatorcontrib>Lu, Zhibin</creatorcontrib><creatorcontrib>Yu, Xueqiao</creatorcontrib><creatorcontrib>Li, Ruiping</creatorcontrib><creatorcontrib>Li, Libo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical science monitor</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Zhenwei</au><au>Wang, Huaiming</au><au>Guo, Guohu</au><au>Li, Xiyao</au><au>Cai, Yongchang</au><au>Tang, Yuxin</au><au>Wang, Yijun</au><au>Li, Jiabao</au><au>Lu, Zhibin</au><au>Yu, Xueqiao</au><au>Li, Ruiping</au><au>Li, Libo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901</atitle><jtitle>Medical science monitor</jtitle><addtitle>Med Sci Monit</addtitle><date>2019-04-02</date><risdate>2019</risdate><volume>25</volume><spage>2386</spage><epage>2396</epage><pages>2386-2396</pages><issn>1643-3750</issn><issn>1234-1010</issn><eissn>1643-3750</eissn><abstract>BACKGROUND Cisplatin-resistant gastric cancer (GC) occurs in patients with GC treated with cisplatin-based chemotherapy, which results in disease progression and early recurrence during the treatment. MATERIAL AND METHODS To understand the initiation and developmental mechanism underlying cisplatin-resistant GC, we developed cisplatin-resistant SGC7901 cells (SGC7901/DDP) from the parental cells (SGC7901/S) by continuous exposure to increasing concentrations of cisplatin and subjected these 2 cell lines to RNA sequencing analysis. The data were verified by quantitative polymerase chain reaction and their functional role was evaluated by cell counting kit 8 assay and cell apoptosis and cell cycle flow cytometric analysis. Bioinformatics analysis was performed to classify the differentially-expressed genes (DEGs) involved in the development of cisplatin resistance. RESULTS In comparison with SGC7901/S cells, SGC7901/DDP cells showed a total of 3165 DEGs (2014 upregulated and 1151 downregulated, fold change ≥2, and adjusted P value <0.001). qRT-PCR confirmed the reliability of the RNA sequencing results. Depletion of the top 5 upregulated mRNAs reversed the resistant index, increased apoptotic SGC7901/DDP cells, and arrested the cells at G2/M phase. Gene ontology analysis revealed that the DEGs mainly regulate metabolic process, immune system, locomotion, cell adhesion, cell growth, cell death, cytoskeleton organization, cell binding, signal transducing activity, and antioxidant activity. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEGs were mainly involved in the PI3K-Akt signaling pathway, Rap1 signaling pathway, proteoglycans in cancer, regulation of actin cytoskeleton, and pathways in cancer. CONCLUSIONS The present study is the first to interrogate mRNAs profiles in human GC cells with cisplatin resistance using RNA sequencing, which may assist in discovering potential therapeutic targets for cisplatin-resistant GC patients.</abstract><cop>United States</cop><pub>International Scientific Literature, Inc</pub><pmid>30938333</pmid><doi>10.12659/MSM.915866</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1643-3750 |
| ispartof | Medical science monitor, 2019-04, Vol.25, p.2386-2396 |
| issn | 1643-3750 1234-1010 1643-3750 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6457324 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Lab/In Vitro Research |
| title | Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T06%3A13%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Next-Generation%20Sequencing%20Analysis%20of%20mRNA%20Profile%20in%20Cisplatin-Resistant%20Gastric%20Cancer%20Cell%20Line%20SGC7901&rft.jtitle=Medical%20science%20monitor&rft.au=Deng,%20Zhenwei&rft.date=2019-04-02&rft.volume=25&rft.spage=2386&rft.epage=2396&rft.pages=2386-2396&rft.issn=1643-3750&rft.eissn=1643-3750&rft_id=info:doi/10.12659/MSM.915866&rft_dat=%3Cpubmed_cross%3E30938333%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/30938333&rfr_iscdi=true |