mRNA expression of tumor-associated genes in canine grade I meningiomas
This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standar...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2019, Vol.81(3), pp.369-372 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | OKABAYASHI, Ken NARITA, Takanori TAKASHIRO, Saki NADAOKA, Sawako KANAI, Shuichiro ITO, Daisuke KITAGAWA, Masato |
| description | This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings. |
| doi_str_mv | 10.1292/jvms.18-0491 |
| format | Article |
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Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings.</description><subject>canine meningioma</subject><subject>Epidermal growth factor receptors</subject><subject>Gene expression</subject><subject>housekeeping genes</subject><subject>Meninges</subject><subject>Polymerase chain reaction</subject><subject>real-time RT-PCR</subject><subject>Surgery</subject><subject>tumor-associated genes</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkd9LHDEQx0NR6tX2rc8l4EsfXJsfm2TzIqhYFcRCaZ9Dkp1bc-wmZ7Ir7X_vHnc9qi8zDPPhwwxfhD5TckaZZt9Wz0M5o01Fak3foQXltapUzfUBWhBNZaWYIEfoQykrQhitpX6PjjiRqlZcL9DN8PPhAsOfdYZSQoo4LfE4DSlXtpTkgx2hxR1EKDhE7G0MEXCXbQv4Dg8wj11Igy0f0eHS9gU-7fox-v39-tfVbXX_4-bu6uK-8pI2Y-W8aB3xyrVgtdNUzCcCEwIkKK8FERJa5pgDL7TQTChJPIWGONUq6aTnx-h8611PboDWQxyz7c06h8HmvybZYF5vYng0XXo2shZUMzILvu4EOT1NUEYzhOKh722ENBXDqNI1bTTXM3ryBl2lKcf5PcMY15KxhsiZOt1SPqdSMiz3x1BiNgmZTUKGNmaT0Ix_-f-BPfwvkhm43AKrMtoO9oDNY_A9bG0NNXxTdtb90j_abCDyFzo5pS0</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>OKABAYASHI, Ken</creator><creator>NARITA, Takanori</creator><creator>TAKASHIRO, Saki</creator><creator>NADAOKA, Sawako</creator><creator>KANAI, Shuichiro</creator><creator>ITO, Daisuke</creator><creator>KITAGAWA, Masato</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190301</creationdate><title>mRNA expression of tumor-associated genes in canine grade I meningiomas</title><author>OKABAYASHI, Ken ; NARITA, Takanori ; TAKASHIRO, Saki ; NADAOKA, Sawako ; KANAI, Shuichiro ; ITO, Daisuke ; KITAGAWA, Masato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-bc5db0c7bdea9b915091e255e6e7c95056ed2b2bec595925760c1e80b7d76b6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>canine meningioma</topic><topic>Epidermal growth factor receptors</topic><topic>Gene expression</topic><topic>housekeeping genes</topic><topic>Meninges</topic><topic>Polymerase chain reaction</topic><topic>real-time RT-PCR</topic><topic>Surgery</topic><topic>tumor-associated genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKABAYASHI, Ken</creatorcontrib><creatorcontrib>NARITA, Takanori</creatorcontrib><creatorcontrib>TAKASHIRO, Saki</creatorcontrib><creatorcontrib>NADAOKA, Sawako</creatorcontrib><creatorcontrib>KANAI, Shuichiro</creatorcontrib><creatorcontrib>ITO, Daisuke</creatorcontrib><creatorcontrib>KITAGAWA, Masato</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKABAYASHI, Ken</au><au>NARITA, Takanori</au><au>TAKASHIRO, Saki</au><au>NADAOKA, Sawako</au><au>KANAI, Shuichiro</au><au>ITO, Daisuke</au><au>KITAGAWA, Masato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>mRNA expression of tumor-associated genes in canine grade I meningiomas</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>81</volume><issue>3</issue><spage>369</spage><epage>372</epage><pages>369-372</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. 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| ispartof | Journal of Veterinary Medical Science, 2019, Vol.81(3), pp.369-372 |
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| language | eng |
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| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | canine meningioma Epidermal growth factor receptors Gene expression housekeeping genes Meninges Polymerase chain reaction real-time RT-PCR Surgery tumor-associated genes |
| title | mRNA expression of tumor-associated genes in canine grade I meningiomas |
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