A helicase links upstream ORFs and RNA structure
Upstream open reading frames (uORFs) in 5′ UTRs of eukaryotic mRNAs are increasingly recognized as important elements that regulate cellular protein synthesis. Since uORFs can start from non-AUG codons, an enormous number of potential uORF initiation sites exists in 5′UTRs. However, only a subset of...
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| Veröffentlicht in: | Current genetics 2019-04, Vol.65 (2), p.453-456 |
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| creator | Jankowsky, Eckhard Guenther, Ulf-Peter |
| description | Upstream open reading frames (uORFs) in 5′ UTRs of eukaryotic mRNAs are increasingly recognized as important elements that regulate cellular protein synthesis. Since uORFs can start from non-AUG codons, an enormous number of potential uORF initiation sites exists in 5′UTRs. However, only a subset of these sites is used and it has been unclear how actual start sites are selected. Studies of the DEAD-box helicase Ded1p from
S. cerevisiae
show that translation of uORFs with non-AUG initiation codons occurs upstream of mRNA structures that emerge with defective Ded1p. The data designate mRNA structure as important determinant for non-AUG initiation sites of uORFs. Ded1p can control this RNA structure and thereby regulate uORF translation. |
| doi_str_mv | 10.1007/s00294-018-0911-z |
| format | Article |
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S. cerevisiae
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S. cerevisiae
show that translation of uORFs with non-AUG initiation codons occurs upstream of mRNA structures that emerge with defective Ded1p. The data designate mRNA structure as important determinant for non-AUG initiation sites of uORFs. Ded1p can control this RNA structure and thereby regulate uORF translation.</description><subject>5' Untranslated Regions</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Codon</subject><subject>Codons</subject><subject>DNA helicase</subject><subject>DNA Helicases - metabolism</subject><subject>Efficiency</subject><subject>Enzymes</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Life Sciences</subject><subject>Meiosis - genetics</subject><subject>messenger RNA</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mini-Review</subject><subject>mRNA</subject><subject>Open Reading Frames</subject><subject>Plant Sciences</subject><subject>Protein Biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - 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metabolism</topic><topic>Efficiency</topic><topic>Enzymes</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Life Sciences</topic><topic>Meiosis - genetics</topic><topic>messenger RNA</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mini-Review</topic><topic>mRNA</topic><topic>Open Reading Frames</topic><topic>Plant Sciences</topic><topic>Protein Biosynthesis</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - chemistry</topic><topic>RNA, Messenger - genetics</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Translation</topic><topic>translation (genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jankowsky, Eckhard</creatorcontrib><creatorcontrib>Guenther, Ulf-Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - 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S. cerevisiae
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| ispartof | Current genetics, 2019-04, Vol.65 (2), p.453-456 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | 5' Untranslated Regions Biochemistry Biomedical and Life Sciences Cell Biology Codon Codons DNA helicase DNA Helicases - metabolism Efficiency Enzymes Gene Expression Regulation, Fungal Life Sciences Meiosis - genetics messenger RNA Microbial Genetics and Genomics Microbiology Mini-Review mRNA Open Reading Frames Plant Sciences Protein Biosynthesis Protein synthesis Proteins Proteomics Ribonucleic acid RNA RNA, Messenger - chemistry RNA, Messenger - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Translation translation (genetics) |
| title | A helicase links upstream ORFs and RNA structure |
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