Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice
The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human im...
Gespeichert in:
| Veröffentlicht in: | Life science alliance 2019-04, Vol.2 (2), p.e201800195 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | e201800195 |
| container_title | Life science alliance |
| container_volume | 2 |
| creator | Matsuda, Masashi Ono, Rintaro Iyoda, Tomonori Endo, Takaho Iwasaki, Makoto Tomizawa-Murasawa, Mariko Saito, Yoriko Kaneko, Akiko Shimizu, Kanako Yamada, Daisuke Ogonuki, Narumi Watanabe, Takashi Nakayama, Manabu Koseki, Yoko Kezuka-Shiotani, Fuyuko Hasegawa, Takanori Yabe, Hiromasa Kato, Shunichi Ogura, Atsuo Shultz, Leonard D Ohara, Osamu Taniguchi, Masaru Koseki, Haruhiko Fujii, Shin-Ichiro Ishikawa, Fumihiko |
| description | The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells. |
| doi_str_mv | 10.26508/lsa.201800195 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6445396</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2202195329</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-326d2d5b93878c3b5c09feba891a84db60434c7b14348d0c7503c7b944646be03</originalsourceid><addsrcrecordid>eNpVUUtPwkAQ3hiNGOTq0fTopTD7bPdiYkClgcBBPW-22wUqfWCXkvjvXQEJJpPMTOabbx4fQncY-kRwiAeF030COAbAkl-gG8IjHmKIosuzuIN6zn0CAPHGOLtGHQqSChzzGzQZt6WugtkkMLYogszubFFvSlttg7wKVsk0jAJdZfsI82Bd1WbtC7P5aPA2TEaDZEqa5WQelLmxt-hqoQtne0ffRR8vz-_DcTidvybDp2loGBfbkBKRkYynksZRbGjKDciFTXUssY5ZlgpglJkoxd7FGZiIA_WpZEwwkVqgXfR44N20aWkz45dtdKE2TV7q5lvVOlf_K1W-Ust6pwRjnErhCR6OBE391Vq3VWXufu_Xla1bpwgB4h9KifTQ_gFqmtq5xi5OYzCovQjKi6BOIviG-_PlTvC_l9Mf0ah_GA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2202195329</pqid></control><display><type>article</type><title>Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Matsuda, Masashi ; Ono, Rintaro ; Iyoda, Tomonori ; Endo, Takaho ; Iwasaki, Makoto ; Tomizawa-Murasawa, Mariko ; Saito, Yoriko ; Kaneko, Akiko ; Shimizu, Kanako ; Yamada, Daisuke ; Ogonuki, Narumi ; Watanabe, Takashi ; Nakayama, Manabu ; Koseki, Yoko ; Kezuka-Shiotani, Fuyuko ; Hasegawa, Takanori ; Yabe, Hiromasa ; Kato, Shunichi ; Ogura, Atsuo ; Shultz, Leonard D ; Ohara, Osamu ; Taniguchi, Masaru ; Koseki, Haruhiko ; Fujii, Shin-Ichiro ; Ishikawa, Fumihiko</creator><creatorcontrib>Matsuda, Masashi ; Ono, Rintaro ; Iyoda, Tomonori ; Endo, Takaho ; Iwasaki, Makoto ; Tomizawa-Murasawa, Mariko ; Saito, Yoriko ; Kaneko, Akiko ; Shimizu, Kanako ; Yamada, Daisuke ; Ogonuki, Narumi ; Watanabe, Takashi ; Nakayama, Manabu ; Koseki, Yoko ; Kezuka-Shiotani, Fuyuko ; Hasegawa, Takanori ; Yabe, Hiromasa ; Kato, Shunichi ; Ogura, Atsuo ; Shultz, Leonard D ; Ohara, Osamu ; Taniguchi, Masaru ; Koseki, Haruhiko ; Fujii, Shin-Ichiro ; Ishikawa, Fumihiko</creatorcontrib><description>The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells.</description><identifier>ISSN: 2575-1077</identifier><identifier>EISSN: 2575-1077</identifier><identifier>DOI: 10.26508/lsa.201800195</identifier><identifier>PMID: 30936185</identifier><language>eng</language><publisher>United States: Life Science Alliance LLC</publisher><subject>Animals ; CD56 Antigen - metabolism ; Cell Differentiation ; Female ; Fetal Blood - cytology ; Gene Knock-In Techniques ; Humans ; Interleukin-15 - blood ; Interleukin-15 - genetics ; Interleukin-7 - blood ; Interleukin-7 - genetics ; Killer Cells, Natural - physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred NOD ; Mice, SCID ; Mice, Transgenic ; Models, Animal ; Thymus Gland - cytology ; Transcriptome ; Transplantation, Heterologous</subject><ispartof>Life science alliance, 2019-04, Vol.2 (2), p.e201800195</ispartof><rights>2019 Matsuda et al.</rights><rights>2019 Matsuda et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-326d2d5b93878c3b5c09feba891a84db60434c7b14348d0c7503c7b944646be03</citedby><cites>FETCH-LOGICAL-c456t-326d2d5b93878c3b5c09feba891a84db60434c7b14348d0c7503c7b944646be03</cites><orcidid>0000-0002-8717-1237 ; 0000-0002-3328-9571 ; 0000-0001-8424-5854</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445396/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445396/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30936185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuda, Masashi</creatorcontrib><creatorcontrib>Ono, Rintaro</creatorcontrib><creatorcontrib>Iyoda, Tomonori</creatorcontrib><creatorcontrib>Endo, Takaho</creatorcontrib><creatorcontrib>Iwasaki, Makoto</creatorcontrib><creatorcontrib>Tomizawa-Murasawa, Mariko</creatorcontrib><creatorcontrib>Saito, Yoriko</creatorcontrib><creatorcontrib>Kaneko, Akiko</creatorcontrib><creatorcontrib>Shimizu, Kanako</creatorcontrib><creatorcontrib>Yamada, Daisuke</creatorcontrib><creatorcontrib>Ogonuki, Narumi</creatorcontrib><creatorcontrib>Watanabe, Takashi</creatorcontrib><creatorcontrib>Nakayama, Manabu</creatorcontrib><creatorcontrib>Koseki, Yoko</creatorcontrib><creatorcontrib>Kezuka-Shiotani, Fuyuko</creatorcontrib><creatorcontrib>Hasegawa, Takanori</creatorcontrib><creatorcontrib>Yabe, Hiromasa</creatorcontrib><creatorcontrib>Kato, Shunichi</creatorcontrib><creatorcontrib>Ogura, Atsuo</creatorcontrib><creatorcontrib>Shultz, Leonard D</creatorcontrib><creatorcontrib>Ohara, Osamu</creatorcontrib><creatorcontrib>Taniguchi, Masaru</creatorcontrib><creatorcontrib>Koseki, Haruhiko</creatorcontrib><creatorcontrib>Fujii, Shin-Ichiro</creatorcontrib><creatorcontrib>Ishikawa, Fumihiko</creatorcontrib><title>Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice</title><title>Life science alliance</title><addtitle>Life Sci Alliance</addtitle><description>The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells.</description><subject>Animals</subject><subject>CD56 Antigen - metabolism</subject><subject>Cell Differentiation</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>Gene Knock-In Techniques</subject><subject>Humans</subject><subject>Interleukin-15 - blood</subject><subject>Interleukin-15 - genetics</subject><subject>Interleukin-7 - blood</subject><subject>Interleukin-7 - genetics</subject><subject>Killer Cells, Natural - physiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Mice, Transgenic</subject><subject>Models, Animal</subject><subject>Thymus Gland - cytology</subject><subject>Transcriptome</subject><subject>Transplantation, Heterologous</subject><issn>2575-1077</issn><issn>2575-1077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtPwkAQ3hiNGOTq0fTopTD7bPdiYkClgcBBPW-22wUqfWCXkvjvXQEJJpPMTOabbx4fQncY-kRwiAeF030COAbAkl-gG8IjHmKIosuzuIN6zn0CAPHGOLtGHQqSChzzGzQZt6WugtkkMLYogszubFFvSlttg7wKVsk0jAJdZfsI82Bd1WbtC7P5aPA2TEaDZEqa5WQelLmxt-hqoQtne0ffRR8vz-_DcTidvybDp2loGBfbkBKRkYynksZRbGjKDciFTXUssY5ZlgpglJkoxd7FGZiIA_WpZEwwkVqgXfR44N20aWkz45dtdKE2TV7q5lvVOlf_K1W-Ust6pwRjnErhCR6OBE391Vq3VWXufu_Xla1bpwgB4h9KifTQ_gFqmtq5xi5OYzCovQjKi6BOIviG-_PlTvC_l9Mf0ah_GA</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Matsuda, Masashi</creator><creator>Ono, Rintaro</creator><creator>Iyoda, Tomonori</creator><creator>Endo, Takaho</creator><creator>Iwasaki, Makoto</creator><creator>Tomizawa-Murasawa, Mariko</creator><creator>Saito, Yoriko</creator><creator>Kaneko, Akiko</creator><creator>Shimizu, Kanako</creator><creator>Yamada, Daisuke</creator><creator>Ogonuki, Narumi</creator><creator>Watanabe, Takashi</creator><creator>Nakayama, Manabu</creator><creator>Koseki, Yoko</creator><creator>Kezuka-Shiotani, Fuyuko</creator><creator>Hasegawa, Takanori</creator><creator>Yabe, Hiromasa</creator><creator>Kato, Shunichi</creator><creator>Ogura, Atsuo</creator><creator>Shultz, Leonard D</creator><creator>Ohara, Osamu</creator><creator>Taniguchi, Masaru</creator><creator>Koseki, Haruhiko</creator><creator>Fujii, Shin-Ichiro</creator><creator>Ishikawa, Fumihiko</creator><general>Life Science Alliance LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8717-1237</orcidid><orcidid>https://orcid.org/0000-0002-3328-9571</orcidid><orcidid>https://orcid.org/0000-0001-8424-5854</orcidid></search><sort><creationdate>20190401</creationdate><title>Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice</title><author>Matsuda, Masashi ; Ono, Rintaro ; Iyoda, Tomonori ; Endo, Takaho ; Iwasaki, Makoto ; Tomizawa-Murasawa, Mariko ; Saito, Yoriko ; Kaneko, Akiko ; Shimizu, Kanako ; Yamada, Daisuke ; Ogonuki, Narumi ; Watanabe, Takashi ; Nakayama, Manabu ; Koseki, Yoko ; Kezuka-Shiotani, Fuyuko ; Hasegawa, Takanori ; Yabe, Hiromasa ; Kato, Shunichi ; Ogura, Atsuo ; Shultz, Leonard D ; Ohara, Osamu ; Taniguchi, Masaru ; Koseki, Haruhiko ; Fujii, Shin-Ichiro ; Ishikawa, Fumihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-326d2d5b93878c3b5c09feba891a84db60434c7b14348d0c7503c7b944646be03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>CD56 Antigen - metabolism</topic><topic>Cell Differentiation</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>Gene Knock-In Techniques</topic><topic>Humans</topic><topic>Interleukin-15 - blood</topic><topic>Interleukin-15 - genetics</topic><topic>Interleukin-7 - blood</topic><topic>Interleukin-7 - genetics</topic><topic>Killer Cells, Natural - physiology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Mice, Transgenic</topic><topic>Models, Animal</topic><topic>Thymus Gland - cytology</topic><topic>Transcriptome</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuda, Masashi</creatorcontrib><creatorcontrib>Ono, Rintaro</creatorcontrib><creatorcontrib>Iyoda, Tomonori</creatorcontrib><creatorcontrib>Endo, Takaho</creatorcontrib><creatorcontrib>Iwasaki, Makoto</creatorcontrib><creatorcontrib>Tomizawa-Murasawa, Mariko</creatorcontrib><creatorcontrib>Saito, Yoriko</creatorcontrib><creatorcontrib>Kaneko, Akiko</creatorcontrib><creatorcontrib>Shimizu, Kanako</creatorcontrib><creatorcontrib>Yamada, Daisuke</creatorcontrib><creatorcontrib>Ogonuki, Narumi</creatorcontrib><creatorcontrib>Watanabe, Takashi</creatorcontrib><creatorcontrib>Nakayama, Manabu</creatorcontrib><creatorcontrib>Koseki, Yoko</creatorcontrib><creatorcontrib>Kezuka-Shiotani, Fuyuko</creatorcontrib><creatorcontrib>Hasegawa, Takanori</creatorcontrib><creatorcontrib>Yabe, Hiromasa</creatorcontrib><creatorcontrib>Kato, Shunichi</creatorcontrib><creatorcontrib>Ogura, Atsuo</creatorcontrib><creatorcontrib>Shultz, Leonard D</creatorcontrib><creatorcontrib>Ohara, Osamu</creatorcontrib><creatorcontrib>Taniguchi, Masaru</creatorcontrib><creatorcontrib>Koseki, Haruhiko</creatorcontrib><creatorcontrib>Fujii, Shin-Ichiro</creatorcontrib><creatorcontrib>Ishikawa, Fumihiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Life science alliance</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuda, Masashi</au><au>Ono, Rintaro</au><au>Iyoda, Tomonori</au><au>Endo, Takaho</au><au>Iwasaki, Makoto</au><au>Tomizawa-Murasawa, Mariko</au><au>Saito, Yoriko</au><au>Kaneko, Akiko</au><au>Shimizu, Kanako</au><au>Yamada, Daisuke</au><au>Ogonuki, Narumi</au><au>Watanabe, Takashi</au><au>Nakayama, Manabu</au><au>Koseki, Yoko</au><au>Kezuka-Shiotani, Fuyuko</au><au>Hasegawa, Takanori</au><au>Yabe, Hiromasa</au><au>Kato, Shunichi</au><au>Ogura, Atsuo</au><au>Shultz, Leonard D</au><au>Ohara, Osamu</au><au>Taniguchi, Masaru</au><au>Koseki, Haruhiko</au><au>Fujii, Shin-Ichiro</au><au>Ishikawa, Fumihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice</atitle><jtitle>Life science alliance</jtitle><addtitle>Life Sci Alliance</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>2</volume><issue>2</issue><spage>e201800195</spage><pages>e201800195-</pages><issn>2575-1077</issn><eissn>2575-1077</eissn><abstract>The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells.</abstract><cop>United States</cop><pub>Life Science Alliance LLC</pub><pmid>30936185</pmid><doi>10.26508/lsa.201800195</doi><orcidid>https://orcid.org/0000-0002-8717-1237</orcidid><orcidid>https://orcid.org/0000-0002-3328-9571</orcidid><orcidid>https://orcid.org/0000-0001-8424-5854</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2575-1077 |
| ispartof | Life science alliance, 2019-04, Vol.2 (2), p.e201800195 |
| issn | 2575-1077 2575-1077 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6445396 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals CD56 Antigen - metabolism Cell Differentiation Female Fetal Blood - cytology Gene Knock-In Techniques Humans Interleukin-15 - blood Interleukin-15 - genetics Interleukin-7 - blood Interleukin-7 - genetics Killer Cells, Natural - physiology Male Mice Mice, Inbred C57BL Mice, Inbred NOD Mice, SCID Mice, Transgenic Models, Animal Thymus Gland - cytology Transcriptome Transplantation, Heterologous |
| title | Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T01%3A58%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20NK%20cell%20development%20in%20hIL-7%20and%20hIL-15%20knockin%20NOD/SCID/IL2rgKO%20mice&rft.jtitle=Life%20science%20alliance&rft.au=Matsuda,%20Masashi&rft.date=2019-04-01&rft.volume=2&rft.issue=2&rft.spage=e201800195&rft.pages=e201800195-&rft.issn=2575-1077&rft.eissn=2575-1077&rft_id=info:doi/10.26508/lsa.201800195&rft_dat=%3Cproquest_pubme%3E2202195329%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2202195329&rft_id=info:pmid/30936185&rfr_iscdi=true |