The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila . This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in th...
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| Veröffentlicht in: | Scientific reports 2019-03, Vol.9 (1), p.5314-12, Article 5314 |
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| creator | Melnikova, Larisa Elizar’ev, Pavel Erokhin, Maksim Molodina, Varvara Chetverina, Darya Kostyuchenko, Margarita Georgiev, Pavel Golovnin, Anton |
| description | Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in
Drosophila
. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described
in vivo
function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw). |
| doi_str_mv | 10.1038/s41598-019-41761-6 |
| format | Article |
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Drosophila
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in vivo
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Drosophila
. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described
in vivo
function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw).</description><subject>38/44</subject><subject>38/77</subject><subject>631/208/199</subject><subject>631/337/572/2102</subject><subject>64/24</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Computational Biology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA-Binding Proteins</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - chemistry</subject><subject>Drosophila Proteins - metabolism</subject><subject>Enhancer Elements, Genetic</subject><subject>Gene Expression Regulation</subject><subject>Gene silencing</subject><subject>Humanities and Social Sciences</subject><subject>Insulator Elements</subject><subject>multidisciplinary</subject><subject>Oocytes</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Proteins</subject><subject>Repressor Proteins - 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genetics</topic><topic>Drosophila - metabolism</topic><topic>Drosophila Proteins - chemistry</topic><topic>Drosophila Proteins - metabolism</topic><topic>Enhancer Elements, Genetic</topic><topic>Gene Expression Regulation</topic><topic>Gene silencing</topic><topic>Humanities and Social Sciences</topic><topic>Insulator Elements</topic><topic>multidisciplinary</topic><topic>Oocytes</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Proteins</topic><topic>Repressor Proteins - chemistry</topic><topic>Repressor Proteins - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Zinc finger proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Melnikova, Larisa</creatorcontrib><creatorcontrib>Elizar’ev, Pavel</creatorcontrib><creatorcontrib>Erokhin, Maksim</creatorcontrib><creatorcontrib>Molodina, Varvara</creatorcontrib><creatorcontrib>Chetverina, Darya</creatorcontrib><creatorcontrib>Kostyuchenko, Margarita</creatorcontrib><creatorcontrib>Georgiev, Pavel</creatorcontrib><creatorcontrib>Golovnin, Anton</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melnikova, Larisa</au><au>Elizar’ev, Pavel</au><au>Erokhin, Maksim</au><au>Molodina, Varvara</au><au>Chetverina, Darya</au><au>Kostyuchenko, Margarita</au><au>Georgiev, Pavel</au><au>Golovnin, Anton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-03-29</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>5314</spage><epage>12</epage><pages>5314-12</pages><artnum>5314</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in
Drosophila
. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described
in vivo
function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw).</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30926937</pmid><doi>10.1038/s41598-019-41761-6</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4898-7118</orcidid><orcidid>https://orcid.org/0000-0003-0008-3814</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38/44 38/77 631/208/199 631/337/572/2102 64/24 Animals Base Sequence Binding Sites Computational Biology Deoxyribonucleic acid DNA DNA-Binding Proteins Drosophila - genetics Drosophila - metabolism Drosophila Proteins - chemistry Drosophila Proteins - metabolism Enhancer Elements, Genetic Gene Expression Regulation Gene silencing Humanities and Social Sciences Insulator Elements multidisciplinary Oocytes Promoter Regions, Genetic Protein Binding Protein Interaction Domains and Motifs Proteins Repressor Proteins - chemistry Repressor Proteins - metabolism Science Science (multidisciplinary) Zinc finger proteins |
| title | The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression |
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