Effect of salidroside on bone marrow haematopoiesis in a mouse model of myelosuppressed anaemia

Abstract This study was designed to investigate the effect of salidroside (SAL) on bone marrow haematopoiesis in a mouse model of myelosuppressed anemia. After the mouse model was established by 60Co γ irradiation and cyclophosphamide, pancytopenia and a sharp reduction in bone marrow stromal cells...

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Veröffentlicht in:Journal of radiation research 2019-03, Vol.60 (2), p.197-203
Hauptverfasser: Chen, Xiaoyan, Fang, Chunjuan
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description Abstract This study was designed to investigate the effect of salidroside (SAL) on bone marrow haematopoiesis in a mouse model of myelosuppressed anemia. After the mouse model was established by 60Co γ irradiation and cyclophosphamide, pancytopenia and a sharp reduction in bone marrow stromal cells and bone marrow haematopoietic stem cells (lineage−Sca1+c-kit+) were observed. This was greatly alleviated by SAL (25 mg/kg, 50 mg/kg, 100 mg/kg) in a dose-dependent manner (50% effective dose value of 35.7 mg/kg and 61.2 mg/kg, respectively), followed by a distinct increment in anti-apoptotic protein Bcl-2. For cell culture in vitro, treatment with SAL resulted in a significant recovery of burst-forming unit–erythroids, and colony-forming unit–granulocyte macrophages on Day 7, and colony-forming unit–erythroids on Day 3, dose-dependently, but not of colony-forming unit–megakaryocyte macrophages. Inoculation of bone marrow cells derived from SAL-administrated donor mice resulted in a 60% survival of recipient mice at the high dose of 100 mg/kg SAL at 2 months after surgery. SAL appeared to be able to stimulate the restoration of bone marrow haemopoietic regulation in myelosuppressed anemia. Based on the downregulation of Fas ligand associated with the expression of Caspase-3 at the protein level, it was suggested that SAL might have an anti-apoptotic effect on bone marrow cells in the Fas-apoptotic pathway of Fas/FasL–caspase-3.
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After the mouse model was established by 60Co γ irradiation and cyclophosphamide, pancytopenia and a sharp reduction in bone marrow stromal cells and bone marrow haematopoietic stem cells (lineage−Sca1+c-kit+) were observed. This was greatly alleviated by SAL (25 mg/kg, 50 mg/kg, 100 mg/kg) in a dose-dependent manner (50% effective dose value of 35.7 mg/kg and 61.2 mg/kg, respectively), followed by a distinct increment in anti-apoptotic protein Bcl-2. For cell culture in vitro, treatment with SAL resulted in a significant recovery of burst-forming unit–erythroids, and colony-forming unit–granulocyte macrophages on Day 7, and colony-forming unit–erythroids on Day 3, dose-dependently, but not of colony-forming unit–megakaryocyte macrophages. Inoculation of bone marrow cells derived from SAL-administrated donor mice resulted in a 60% survival of recipient mice at the high dose of 100 mg/kg SAL at 2 months after surgery. SAL appeared to be able to stimulate the restoration of bone marrow haemopoietic regulation in myelosuppressed anemia. Based on the downregulation of Fas ligand associated with the expression of Caspase-3 at the protein level, it was suggested that SAL might have an anti-apoptotic effect on bone marrow cells in the Fas-apoptotic pathway of Fas/FasL–caspase-3.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1093/jrr/rry093</identifier><identifier>PMID: 30462331</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Anemia ; Anemia - drug therapy ; Animals ; Anopheles ; Apoptosis ; Bone marrow ; Bone Marrow - drug effects ; Bone Marrow - pathology ; Bone Marrow Transplantation ; Care and treatment ; Cell Count ; Cell Lineage ; Cell Proliferation - drug effects ; Cyclophosphamide ; Disease Models, Animal ; Glucosides ; Glucosides - chemistry ; Glucosides - pharmacology ; Glucosides - therapeutic use ; Health aspects ; Hematopoiesis ; Hematopoiesis - drug effects ; Hematopoietic stem cells ; Hematopoietic Stem Cells - drug effects ; Hematopoietic Stem Cells - metabolism ; Immunosuppression ; Macrophages ; Male ; Medical examination ; Mice ; Pancytopenia - drug therapy ; Phenols - chemistry ; Phenols - pharmacology ; Phenols - therapeutic use ; Regular Paper ; Stem cells ; Survival Analysis</subject><ispartof>Journal of radiation research, 2019-03, Vol.60 (2), p.197-203</ispartof><rights>The Author(s) 2018. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. 2018</rights><rights>The Author(s) 2018. 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After the mouse model was established by 60Co γ irradiation and cyclophosphamide, pancytopenia and a sharp reduction in bone marrow stromal cells and bone marrow haematopoietic stem cells (lineage−Sca1+c-kit+) were observed. This was greatly alleviated by SAL (25 mg/kg, 50 mg/kg, 100 mg/kg) in a dose-dependent manner (50% effective dose value of 35.7 mg/kg and 61.2 mg/kg, respectively), followed by a distinct increment in anti-apoptotic protein Bcl-2. For cell culture in vitro, treatment with SAL resulted in a significant recovery of burst-forming unit–erythroids, and colony-forming unit–granulocyte macrophages on Day 7, and colony-forming unit–erythroids on Day 3, dose-dependently, but not of colony-forming unit–megakaryocyte macrophages. Inoculation of bone marrow cells derived from SAL-administrated donor mice resulted in a 60% survival of recipient mice at the high dose of 100 mg/kg SAL at 2 months after surgery. SAL appeared to be able to stimulate the restoration of bone marrow haemopoietic regulation in myelosuppressed anemia. Based on the downregulation of Fas ligand associated with the expression of Caspase-3 at the protein level, it was suggested that SAL might have an anti-apoptotic effect on bone marrow cells in the Fas-apoptotic pathway of Fas/FasL–caspase-3.</description><subject>Anemia</subject><subject>Anemia - drug therapy</subject><subject>Animals</subject><subject>Anopheles</subject><subject>Apoptosis</subject><subject>Bone marrow</subject><subject>Bone Marrow - drug effects</subject><subject>Bone Marrow - pathology</subject><subject>Bone Marrow Transplantation</subject><subject>Care and treatment</subject><subject>Cell Count</subject><subject>Cell Lineage</subject><subject>Cell Proliferation - drug effects</subject><subject>Cyclophosphamide</subject><subject>Disease Models, Animal</subject><subject>Glucosides</subject><subject>Glucosides - chemistry</subject><subject>Glucosides - pharmacology</subject><subject>Glucosides - therapeutic use</subject><subject>Health aspects</subject><subject>Hematopoiesis</subject><subject>Hematopoiesis - drug effects</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Immunosuppression</subject><subject>Macrophages</subject><subject>Male</subject><subject>Medical examination</subject><subject>Mice</subject><subject>Pancytopenia - drug therapy</subject><subject>Phenols - chemistry</subject><subject>Phenols - pharmacology</subject><subject>Phenols - therapeutic use</subject><subject>Regular Paper</subject><subject>Stem cells</subject><subject>Survival Analysis</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>EIF</sourceid><recordid>eNp9kVtrFTEUhYMo9lh98QdIQAQRTpuc3JoXoZR6gYIv-hwyyU6bMjMZk5nK-ffuw9RiQSQPCcm3Fll7EfKasxPOrDi9rfW01j0en5ANF9JuLVfmKdkwiWfBNDsiL1q7ZWxnmGLPyZFgUu-E4BviLlOCMNOSaPN9jrW0HIGWkXZlBDr4WssveuNh8HOZSoaWG80j9XQoS0OgROgP6mEPfWnLNFVoDSL1I2qyf0meJd83eHW_H5Mfny6_X3zZXn37_PXi_GobpLXzVielZEo6ma6LxgcFIRoZIg-26zBGZ2IHkoONKoYUldXJmxTEmTeRiwTimHxcfaelGyAGGOfqezfVjBH2rvjsHr-M-cZdlzunpWA7pdHg_b1BLT8XaLMbcgvQ934ETOp2XGilhOQH9O2KXvseXB5TQcdwwN255ppJo88sUif_oHBFnEvA4aaM948EH1ZBwBJahfTwe87coWiHRbu1aITf_J33Af3TLALvVqAs0_-MfgMdzbSy</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Chen, Xiaoyan</creator><creator>Fang, Chunjuan</creator><general>Oxford University Press</general><scope>TOX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0244-9528</orcidid></search><sort><creationdate>20190301</creationdate><title>Effect of salidroside on bone marrow haematopoiesis in a mouse model of myelosuppressed anaemia</title><author>Chen, Xiaoyan ; Fang, Chunjuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-6f554ff6f7bbd7ac5ecd74cd1c9bb044b7dbe41e9d5dcfd596fa7fc38a7d13fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anemia</topic><topic>Anemia - drug therapy</topic><topic>Animals</topic><topic>Anopheles</topic><topic>Apoptosis</topic><topic>Bone marrow</topic><topic>Bone Marrow - drug effects</topic><topic>Bone Marrow - pathology</topic><topic>Bone Marrow Transplantation</topic><topic>Care and treatment</topic><topic>Cell Count</topic><topic>Cell Lineage</topic><topic>Cell Proliferation - drug effects</topic><topic>Cyclophosphamide</topic><topic>Disease Models, Animal</topic><topic>Glucosides</topic><topic>Glucosides - chemistry</topic><topic>Glucosides - pharmacology</topic><topic>Glucosides - therapeutic use</topic><topic>Health aspects</topic><topic>Hematopoiesis</topic><topic>Hematopoiesis - drug effects</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Immunosuppression</topic><topic>Macrophages</topic><topic>Male</topic><topic>Medical examination</topic><topic>Mice</topic><topic>Pancytopenia - drug therapy</topic><topic>Phenols - chemistry</topic><topic>Phenols - pharmacology</topic><topic>Phenols - therapeutic use</topic><topic>Regular Paper</topic><topic>Stem cells</topic><topic>Survival Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xiaoyan</creatorcontrib><creatorcontrib>Fang, Chunjuan</creatorcontrib><collection>OUP_牛津大学出版社OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xiaoyan</au><au>Fang, Chunjuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of salidroside on bone marrow haematopoiesis in a mouse model of myelosuppressed anaemia</atitle><jtitle>Journal of radiation research</jtitle><addtitle>J Radiat Res</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>60</volume><issue>2</issue><spage>197</spage><epage>203</epage><pages>197-203</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>Abstract This study was designed to investigate the effect of salidroside (SAL) on bone marrow haematopoiesis in a mouse model of myelosuppressed anemia. After the mouse model was established by 60Co γ irradiation and cyclophosphamide, pancytopenia and a sharp reduction in bone marrow stromal cells and bone marrow haematopoietic stem cells (lineage−Sca1+c-kit+) were observed. This was greatly alleviated by SAL (25 mg/kg, 50 mg/kg, 100 mg/kg) in a dose-dependent manner (50% effective dose value of 35.7 mg/kg and 61.2 mg/kg, respectively), followed by a distinct increment in anti-apoptotic protein Bcl-2. For cell culture in vitro, treatment with SAL resulted in a significant recovery of burst-forming unit–erythroids, and colony-forming unit–granulocyte macrophages on Day 7, and colony-forming unit–erythroids on Day 3, dose-dependently, but not of colony-forming unit–megakaryocyte macrophages. Inoculation of bone marrow cells derived from SAL-administrated donor mice resulted in a 60% survival of recipient mice at the high dose of 100 mg/kg SAL at 2 months after surgery. SAL appeared to be able to stimulate the restoration of bone marrow haemopoietic regulation in myelosuppressed anemia. Based on the downregulation of Fas ligand associated with the expression of Caspase-3 at the protein level, it was suggested that SAL might have an anti-apoptotic effect on bone marrow cells in the Fas-apoptotic pathway of Fas/FasL–caspase-3.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30462331</pmid><doi>10.1093/jrr/rry093</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0244-9528</orcidid><oa>free_for_read</oa></addata></record>
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subjects Anemia
Anemia - drug therapy
Animals
Anopheles
Apoptosis
Bone marrow
Bone Marrow - drug effects
Bone Marrow - pathology
Bone Marrow Transplantation
Care and treatment
Cell Count
Cell Lineage
Cell Proliferation - drug effects
Cyclophosphamide
Disease Models, Animal
Glucosides
Glucosides - chemistry
Glucosides - pharmacology
Glucosides - therapeutic use
Health aspects
Hematopoiesis
Hematopoiesis - drug effects
Hematopoietic stem cells
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - metabolism
Immunosuppression
Macrophages
Male
Medical examination
Mice
Pancytopenia - drug therapy
Phenols - chemistry
Phenols - pharmacology
Phenols - therapeutic use
Regular Paper
Stem cells
Survival Analysis
title Effect of salidroside on bone marrow haematopoiesis in a mouse model of myelosuppressed anaemia
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